In-Vitro Alpha Amylase Inhibition and Antioxidant Activities of Leaves Extract of Sundanese Traditional Salad (Lalapan) from Indonesia

Abstract

This research aims to evaluate the α-amylase inhibitory activity and antioxidant potential of leaves extract of Gandaria (Bouea macrophylla Griff.), Basil (Ocimum africanum Lour.), Pohpohan (Pilea melastomoides (Poir.) Wedd.), and Lettuce (Lactuca sativa L.) as common vegetables used in Sundanese traditional salad. Extraction methods used were traditional and maceration methods. Phytochemical screening was used to determine the phytochemical component qualitatively. Quantitative estimation of phenols (Folin-Ciocalteu method), flavonoids (AlCl 3 colorimetric assay), total antioxidant activity along with free radical scavenging activity (DPPH) was also carried out. Soluble starch was used as substrate for analysis of α-amylase inhibitory activity, which is calculated by IC 50 value. Phytochemical screening revealed the presence of phytochemical constituents like flavonoids, tannins, saponins, and polyphenols compound in both extraction methods. The results showed that the value of Total Phenolic Content (TPC), Total Flavonoid Content (TFC), antioxidant activity, and α-amylase inhibitory in both extraction were significantly different for each leaves extracts (p<0.05). The gandaria leaves extracted with maceration method had the highest value among the leaves analysed. The value of TPC, TFC, antioxidant activity were 364.56±65.97 mg Gallic Acid Equivalent (GAE)/g extract, 70.2±10.54 mg Quercetin (QE)/g extract, and 35 µg/ml of IC 50 , respectively. The extract of gandaria maceration leaves exhibited significant α-amylase inhibitory activity with an IC 50 value of 60 µg/ml. Various pharmacologically active compounds were found in the local vegetables, thus they can be rich sources of antioxidants and α-amylase inhibitor activity. Hence, could be developed as vegetables based functional food products.

Development of Functional Beverage with Antioxidant Properties using Germinated Red Rice and Tempeh Powder Mixture

“…The GRR single beverage had the total phenolics content and antioxidant activity as high as in the 2:1 mix and higher than that of a single tempeh beverage. There was a potential positive relationship between the total phenolics content and the antioxidant activity of the plant samples as was also reported by Wahyuni et al (2020) in the leaves extrats of Sundanese traditional salad.…”

Identification of Amylase-Producing Thermophilic Bacteria through 16sRNA Gene Analysis: MGH3 Strain

“…Bacillus licheniformis is a bacterium originating from France that can produce antibiotics, biochemical compounds, and various industrial enzymes such as amylase (Ray et al, 2008). Amylase is an enzyme that catalyzes the hydrolysis of α-1,4-glycosidic bonds in starch to glucose (Wahyuni et al, 2020). Amylase is generally a metalloenzyme that requires calcium and chloride ions for activity and maintains the stability and structural integrity of amylase (Nandhany et al, 2020).…”

The Evolution of Pharmacological Activities Bouea macrophylla Griffith In Vivo and In Vitro Study: A Review

“…B. macrophylla Griffith reported high phenolic compound levels that have possible antioxidant activity, as described in Table 1. The antioxidant activity of B. macrophylla Griffith has been explored in all parts of the plant, demonstrating that this species' seed [3,40], fruit [2,15,19], and leaves [17,18,[20][21][22] have antioxidant activity. Although all the experiments are in vitro, these findings give essential information about possible mechanisms of B. macrophylla Griffith.…”

“…Therefore, the plum mango leaf extract with the highest amylase inhibition had the highest antioxidant activity, total phenol content (TPC), and total flavonoid content (TFC). The extract was prepared from plum mango leaves by the maceration extraction method [20]. Adequate blood sugar maintenance can be obtained from natural sources, such as bioactive compounds in plants that can act as amylase inhibitors [120].…”

“…They are reported to have various pharmacological activities such as antioxidant, antimicrobial, anticancer, and antihyperglycemic [10][11][12][13][14]. Related to these data, plum mango has been reported to have various pharmacological activities, including antioxidant [2,3,[15][16][17][18][19][20][21][22], anticancer [3,[23][24][25][26][27], antimicrobial [3,28,29], antihyperglycemic [15,16,20,30], antiphotoaging [30], and can increase vegetable intake and increase the concentration of beta carotene in the blood [31]. By virtue of the use of B. macrophylla Griffith as a herbal medicine and the lack of widespread use of this plant due to the limited information about its therapeutic benefits and compound content, we present this review of the pharmacological activities of B. macrophylla Griffith.…”