In-vitro alpha amylase inhibitory activity of the leaf extracts of Adenanthera pavonina

Wickramaratne, M. Nirmali; Punchihewa, J Chamini; Wickramaratne, D. B. M.

doi:10.1186/s12906-016-1452-y

Cited by 205 publications

(134 citation statements)

References 10 publications

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“…The IC 50 value of acarbose was calculated from the dose-response curves (not given in this paper). The results indicated that the IC 50 value of acarbose was 20 μ g/mL, which was close to the value reported by Wickramaratne et al [8]. Therefore, the concentration of MT was set by taking the ratio between 100 mg of one dose of acarbose and 50 mg of one dose of MT.…”

Section: Methodssupporting

confidence: 82%

Characterization of α-Glucosidase Inhibitor/Cyclodextrin Complex Prepared by Freeze-Drying

Inoue

Narumi

Mitsumori

et al. 2018

Journal of Pharmaceutics

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Miglitol (MT) is an α-glucosidase inhibitor with a postmeal blood glucose level lowering effect that is used to treat type 2 diabetes. In addition, α-cyclodextrin (αCD) has been reported to inhibit increases in postmeal blood glucose. The aim of this study was to prepare a freeze-dried product (FD) composed of MT and αCD or γCD (molar ratio of MT/αCD = 1/1, MT/γCD = 1/1) and to evaluate the physicochemical properties and biological activity of the FD. The PXRD profile of FD exhibited a halo pattern, and characteristic peaks derived from MT, αCD, and γCD were not observed. The TG-DTA results for FD indicated an increased weight loss temperature and the absence of an endothermic peak for MT. The NIR absorption spectrum measurement suggested an intermolecular interaction between MT and αCD or γCD in the FD. 1H-1H NOESY NMR spectroscopy (D2O) revealed an intermolecular interaction in the FD. The results of the α-glucosidase activity inhibition test and the α-amylase activity inhibition test indicated that the FD exhibited the same inhibition rate as MT alone and the effects of MT were not altered by the freeze-drying method.

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Section: Methodssupporting

confidence: 82%

Characterization of α-Glucosidase Inhibitor/Cyclodextrin Complex Prepared by Freeze-Drying

Inoue

Narumi

Mitsumori

et al. 2018

Journal of Pharmaceutics

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“…The data obtained suggest that antioxidant activity could be related to the sulfation degree and probably the mechanism of action associated with the intrinsic hydrogen-donating ability of sulfate groups. Wickramaratne et al (2016) demonstrated the antioxidant activity by DPPH scavenging property, and the total phenolic content of different solvent extracts of A. pavonina leaves. The EtOAc fraction of A. pavonina leaves showed the highest total phenolic content (34.…”

Section: Antioxidant Activitymentioning

confidence: 94%

“…Zeid et al (2012) reported its use as a tonic. These reports of traditional use were most common in Asian countries (Wickramaratne et al, 2016;Koodalingam et al, 2015;Dholvitayakhun et al, 2012;Arshad et al, 2010).…”

Section: Ethnomedicinal Usementioning

confidence: 99%

“…Some studies have been conducted to evaluate the toxicity of derivatives from A. pavonina. Leaf extracts of this plant were screened for toxicity to brine shrimp Artemia salina and presented absence of cytotoxicity (Wickramaratne et al, 2016). However, the root and stem bark extracts showed cytotoxicity against A. salina (Rodrigo et al, 2007;Zeid et al, 2012).…”

Section: Toxicity Studiesmentioning

confidence: 99%

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Advances in the research of Adenanthera pavonina: From traditional use to intellectual property

GeronÃ§o¹,

Melo²,

Barros³

et al. 2020

J. Med. Plants Res.

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Adenanthera pavonina, an Asian native leguminous, is a well known highly used plant in traditional medicine. Such broad herbal medicine applications motivated several researchers to study its chemical composition, mainly of its leaves, seeds, and barks, besides the pharmacological effects aiming to demonstrate scientific evidence of its empirical uses. Most studies have been conducted with plant derivatives, and more recent studies have focused on isolated compounds. The phytochemical screening revealed various secondary metabolites with diverse biological effects, including flavonoids, alkaloids, saponins, tannins, steroids, triterpenoids, polyphenols, anthraquinones, coumarins, glycosides and polysaccharides. Experimental studies of this plant have shown numerous pharmacological activities such as antidiarrheal, anti-inflammatory, antinociceptive, antioxidant, antimicrobial, anticancer, and others. Advances in the studies of phytochemistry and biological activity on this plant species over the last years led to the increasing interest of researchers in the protection of inventions focusing on its cosmetic, pharmaceutical and food applicability. This review summarizes the tremendous therapeutic and technological potential of A. pavonina that should be explored, opening new perspectives for future researches and the development of new products.

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“…[15][16][17][18][19] In this study, the existence and contents of ten flavonoids were analyzed in the extract of lotus leaf. Centrifugal ultrafiltration combined with high performance liquid chromatography (HPLC) was performed for the screening and analysis of alpha-amylase inhibitors.…”

Section: Introductionmentioning

confidence: 99%

Screening and Binding Analysis of Flavonoids with Alpha-Amylase Inhibitory Activity from Lotus Leaf

Liao¹,

Chen²,

Liu³

et al. 2017

J. Braz. Chem. Soc.

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Lotus leaf is gaining growing popularity due to its various benefits and widely usage. In this paper, ten flavonoids in lotus leaf extract were analyzed by high performance liquid chromatography (HPLC). Centrifugal ultrafiltration combined liquid chromatography was used to screen alphaamylase inhibitors from ten flavonoids mixture and the binding degrees ranged from 2.34 to 94.1%. The alpha-amylase inhibition and the 1,1-diphenyl-2-picrylhydrazyl (DPPH) antioxidant activity of ten flavonoids were verified as well. Apigenin, kaempferol and isorhamnetin with relatively higher binding degrees showed higher inhibition on alpha-amylase as well. The interactions between these flavonoids and alpha-amylase were investigated by spectroscopic method. As a result, the fluorescence quenching could be considered as static quenching because the quenching rate constant (K q ) values were higher than 2.0 × 10 10 L mol -1 s -1. The binding constants (log 10 K a ) of ten flavonoids were in the range of 5.971 to 7.417 L mol -1 . The hydrogenation of C 2 =C 3 double bond of apigenin and quercetin decreased the affinity for alpha-amylase (1.92-and 2.82-fold). The hydroxylation on 3 and 3' position decreased the affinity for alpha-amylase. Moreover, the glycosylation with different sugar moiety improved in varying degrees of affinity. The hydrogen bond force might be important in the binding between alpha-amylase and flavonoids.

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In-vitro alpha amylase inhibitory activity of the leaf extracts of Adenanthera pavonina

Cited by 205 publications

References 10 publications

Characterization of α-Glucosidase Inhibitor/Cyclodextrin Complex Prepared by Freeze-Drying

Characterization of α-Glucosidase Inhibitor/Cyclodextrin Complex Prepared by Freeze-Drying

Advances in the research of Adenanthera pavonina: From traditional use to intellectual property

Screening and Binding Analysis of Flavonoids with Alpha-Amylase Inhibitory Activity from Lotus Leaf

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