In vitro amoebicidal activity of four Peucedanum species on Acanthamoeba castellanii cysts and trophozoites

Abstract

Amoebic keratitis is difficult to treat without total efficacy in some patients because of cysts, which is less susceptible than trophozoites to the usual treatments. The aim of this study is to evaluate the in vitro amoebicidal activity of the methanolic extracts of Peucedanum caucasicum, Peucedanum palimbioides, Peucedanum chryseum, and Peucedanum longibracteolatum, which are endemic in Turkish flora except P. caucasicum. Extracts were evaluated for their amoebicidal activities using an inverted light microscope. In the presence of methanolic extracts (ranging from 1.0 to 32.0 mg/ml), numbers of the viable Acanthamoeba castellani trophozoites and cysts were determined during the experimental process (72nd hour). All of the extracts showed a time and dose-dependent amoebicidal action on the trophozoites and cysts. Among the extracts tested, P. longibracteolatum showed the strongest amoebicidal effect on the trophozoites and cysts. In the case of 32 mg/ml concentration of extract, no viable trophozoites or cysts were determined between 24th and 72nd hour. Similar results were obtained from the extract at 16.0 mg/ml concentration against trophozoites. At this concentration value, number of viable cysts was determined as 10.6 ± 2.1 in the 24th hour. In the presence of 8.0 mg/ml extract solution, no viable trophozoites were determined in the 48th hour. At the same concentration, 51% of the cysts were killed by the extract in the 72nd hour. As expected, cysts were found more resistant to the extracts than the trophozoites.

Amoebicidal activity of the rhizomes and aerial parts of Allium sivasicum on Entamoeba histolytica

“…These include antibiotics as well as antifungal, antiviral, antiprotozoal, antipsychotic, and anticancer agents (Mattana et al 2004;Osato et al 1991;Schuster and Visvesvara 1998;Walochnik et al 2002). Amebicidal activities of some plant species have previously been reported by our research team (Tepe et al 2011Goze et al 2009;Akin Polat et al 2007a, b, 2008Malatyali et al 2012a, b;Degerli et al 2012).…”

“…In this context, amebicidal activities of some plant species have been reported by our research team (Tepe et al 2011Goze et al 2009;Akin Polat et al 2007a, b, 2008Malatyali et al 2012a, b;Degerli et al 2012). Drugs of natural origin have already been used to treat other parasitic diseases (Arrieta et al 2001;Kayser et al 2003;Said Fernández et al 2005).…”

In vitro amoebicidal activities of Satureja cuneifolia and Melissa officinalis on Acanthamoeba castellanii cysts and trophozoites

“…These include antibiotics as well as antifungal, antiviral, antiprotozoal, antipsychotic, and anticancer agents (Mattana et al 2004;Osato et al 1991;Schuster and Visvesvara 1998;Walochnik et al 2002). On the other hand, amoebicidal activities of some plant species have previously been reported by our research team (Tepe et al 2011a, b;Goze et al 2009;Akin Polat et al 2007a, b, 2008Degerli et al 2011;Malatyali et al 2011).…”

Antiparasitic Efficacy of Artemisia ludoviciana Nutt. (Asteraceae) Essential Oil for Acanthamoeba castellanii, Leishmania infantum and Trichomonas vaginalis

“…et Heldr. and P. longibracteolatum Parolly and Nordt at 32 mg/mL, 41 Origanum syriacum L. and O. laevigatum Boiss. at 32.0 mg/mL.…”