In vitro amplification of the 16S rRNA genes from Mycoplasma bovis and Mycoplasma agalactiae by PCR

González, Yleana R. Chávez; Bascuñana, C R; Bölske, Göran; Mattsson, Jens G.; Molina, Carmen; Johansson, Karl‐Erik

doi:10.1016/0378-1135(95)00058-i

Cited by 100 publications

(50 citation statements)

References 17 publications

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“…On the other hand, a PCR system for Mycoplasma bovis [2] was also used to identify nine field-captured strains (Table 3), and obtained the same results that were obtained with the serological examination (data not shown). Because the M. bovis-PCR system was already established [2] and the share of M. bovis infection in cattle with respiratory symptoms could not be ignored, we attempted to extend this system to three other pathogenic bovine mycoplasmas (M. alkalescens, M. bovirhinis and M. bovigenitalium), which are often isolated from both adults and calves with respiratory symptoms in Japan.…”

Section: Discussionsupporting

confidence: 64%

“…The mucus was then diluted to 100 ml with transport medium consisting of 20% horse serum and 0.5 mg/ml of ampicillin in PBS [2]. Mycoplasmas whose counts were already known were mixed into 1 ml of the diluted mucus solution.…”

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confidence: 99%

“…However, the former techniques are time consuming and the latter are, in some cases, not sensitive enough. Two molecular biological techniques for detecting bovine mycoplasmas (including the M. mycoides cluster) are hybridization with a randomly cloned DNA fragment [12] and a PCR system that was originally developed for detection of M. bovis using the gene for 16S rRNA [2,4,6].…”

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confidence: 99%

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In Vitro Amplificatin of the 16S rRNA Genes from Mycoplasma bovirhinis, Mycoplasma alkalescens and Mycoplasma bovigenitalium by PCR.

et al. 1998

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ABSTRACT. Polymerase chain reaction (PCR) systems were developed for detection of 3 pathogenic bovine mycoplasmas, Mycoplasma alkalescens (Mak), M. bovigenitalium (Mbg) and M. bovirhinis (Mbr). The primers were selected from the sequences of the 16S rRNA from each of the mycoplasmas. Neither the Mbg-PCR system nor the Mbr-PCR system showed cross-amplification among 24 bovine, caprine-ovine and human mycoplasma species (including Acholeplasma and Ureaplasma species) tested. The Mak-PCR system showed cross-amplification with M. canadens ATCC29418 T (T=type strain). The sensitivity of each PCR system to detect the proper mycoplasma was 10 3 colony forming units (CFU) when a pure culture was tested, and 2 × 10 3 CFU when the mycoplasma culture was spiked into a calf nasal swab sample. The target mycoplasmas in a clinical nasal swab sample that could be detected by the direct plate method could also be detected by these PCR systems. -KEY WORDS: bovine mycoplasma, Mycoplasma alkalescens, M. bovigenitalium, M. bovirhinis, PCR

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Section: Discussionsupporting

confidence: 64%

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confidence: 99%

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confidence: 99%

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In Vitro Amplificatin of the 16S rRNA Genes from Mycoplasma bovirhinis, Mycoplasma alkalescens and Mycoplasma bovigenitalium by PCR.

et al. 1998

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“…One of the options is the use of genetic probes complementary to chromosomal DNA or 16S RNA segments (Mattson et al 1991;Dedieu et al 1992;Tola et al 1994). Although the specificity of this technique is high and DNA probes mean a considerable advance in the diagnosis of mycoplasma infections, efforts in research have recently aimed at the development of PCR techniques which are much more sensitive (Dedieu et al 1995;Tola et al 1996 a,b;Tola et al 1997;Bergonier et al 1996;Chavez et al 1995;Subramaniam et al 1998;Greco et al 2001). So far, however, no standard PCR procedures effective for routine diagnosis have been available (Bashirudin et al 2004).…”

Section: Discussionmentioning

confidence: 99%

Detection of Mycoplasma agalactiae by Polymerase Chain Reaction in Jordanian Sheep and Goat Herds

et al. 2007

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Zendulková D., A. Madanat, P. Lány, K. Rosenbergová, Z. Pospí‰i,: Detection of Mycoplasma agalactiae by Polymerase Chain Reaction in Jordanian Sheep and Goat Herds. Acta Vet Brno 0000, 00: 71-77The aim of the study was to ascertain whether sheep and goats from selected Jordanian herds were infected with Mycoplasma agalactiae, the most common aetiological agent of contagious agalactia of sheep and goats. All examined animals showed clinical signs of disease at the time of sample collection. The group included 35 animals, 15 sheep and 20 goats. For microbiological examination, a total of 107 swabs were taken from conjunctival, nasal, vaginal or preputial mucosae and from the external auditory canal. Identification of the species isolated was carried out by a polymerase chain reaction. Of the 35 animals, 21 (4 sheep and 17 goats) tested positive for Mycoplasma agalactiae. These results confirmed our assumption that this mycoplasma species is present in Jordanian herds and, for the first time, provided evidence that contagious agalactia of sheep and goats occurs in Jordan.

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“…capricolum, some of them combining an amplification step with two pairs of different primers and an identification step based on the analysis of restriction profiles of PCR products (Dedieu et al 1995;Tola et al 1996ab;Tola et al 1997). Other PCR techniques are based on 16S rRNA (Bergonier et al 1996b;Chavez et al 1995) or on amplification of segments of the uvrC gene (Subramaniam et al 1998). The most recent approach described by Greco et al (2001) is the multiplex-PCR.…”

Section: Diagnosismentioning

confidence: 99%

Contagious Agalactia of Sheep and Goats. A Review

Madanat¹,

Zendulková²,

Pospíšil³

2001

Acta Vet. Brno

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In vitro amplification of the 16S rRNA genes from Mycoplasma bovis and Mycoplasma agalactiae by PCR

Cited by 100 publications

References 17 publications

In Vitro Amplificatin of the 16S rRNA Genes from Mycoplasma bovirhinis, Mycoplasma alkalescens and Mycoplasma bovigenitalium by PCR.

In Vitro Amplificatin of the 16S rRNA Genes from Mycoplasma bovirhinis, Mycoplasma alkalescens and Mycoplasma bovigenitalium by PCR.

Detection of Mycoplasma agalactiae by Polymerase Chain Reaction in Jordanian Sheep and Goat Herds

Contagious Agalactia of Sheep and Goats. A Review

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