In vitro analysis of oocyte cumulus complex pickup rate in the hamster Mesocricetus auratus

Huang, Susan S.; Driessen, Natalie; Knöll, Martin; Talbot, Prudence

doi:10.1002/(sici)1098-2795(199707)47:3<312::aid-mrd10>3.0.co;2-#

Cited by 21 publications

(23 citation statements)

References 19 publications

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“…An assay previously developed in our laboratory was used to determine whether cyanide could affect OPR on hamster infundibula in vitro [6,26]. OCCs recovered from mature ovarian follicles and stained with methylene blue dye were placed on infundibula in EBSS-HA.…”

Section: Effect Of Cyanide On Occ Pick-up Rate (Opr) In Vitromentioning

confidence: 99%

“…In hamsters, the outer surface of the infundibulum is covered mainly by ciliated cells [3]. After ovulation, these ciliated cells pick up the oocyte cumulus complex (OCC) and transfer it to the ampulla, where fertilization occurs [4][5][6].…”

Section: Introductionmentioning

confidence: 99%

“…This inhibition is reversed or partially reversed when smoke solutions are replaced with control medium. OCC pick-up rate (OPR), which can be quantified in vitro using hamster infundibular explants [6], also decreases during in vitro exposure to both MS and SS smoke solutions [13]. The inhibition of OPR by smoke solutions is probably due in part to an effect on CBF However, unlike CBF, OPR does not recover after washout of smoke solutions, suggesting that the mechanism of action of smoke solutions on the oviduct involves more than just inhibition of CBE Cigarette smoke is a complex mixture of over 4000 chemicals [14], and cigarette smoke solutions made in physiological media likewise contain numerous different types of chemicals that include the more water-soluble components of cigarette smoke.…”

Section: Introductionmentioning

confidence: 99%

“…The purpose of this study was to identify the ciliotoxic reagent(s] in MS and SS smoke solutions that inhibit CBF and OPR using in vitro assays [6,20]. Compounds that were tested were chosen because in most previous reports they were found to be ciliotoxic in other systems.…”

Section: Introductionmentioning

confidence: 99%

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Identification of Cigarette Smoke Components That Alter Functioning of Hamster (Mesocricetus auratus) Oviducts In Vitro1

Talbot¹,

DiCarlantonio

Knöll

et al. 1998

Biology of Reproduction

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Previous studies have shown that solutions of mainstream and sidestream cigarette smoke decrease both ciliary beat frequency and oocyte cumulus complex pick-up rate of hamster oviducts in vitro. The purpose of this study was to identify the component(s) in smoke that produces these effects. Chemicals reported to be ciliotoxic in other systems were tested on hamster infundibula at various concentrations to determine whether a dose-response inhibition of ciliary beat frequency occurred. In addition, the concentration of each test component was measured in mainstream and sidestream smoke solutions (whole, particulate, and gas phase). All test components (acrolein, formaldehyde, phenol, acetaldehyde, and potassium cyanide [KCN]) inhibited ciliary beat frequency in a dose-dependent manner. Inhibition of ciliary beat frequency was at least partially reversible for all test compounds except acrolein. The concentrations of acrolein, formaldehyde, and phenol that were required to inhibit beat frequency were at least 3-50 times higher than their corresponding concentrations in smoke solutions. In contrast, cyanide was present in all smoke solutions at concentrations sufficient to inhibit ciliary beat frequency. Cilia on the outer surface of the infundibulum function in picking up the oocyte cumulus complex. Oocyte cumulus complex pick-up rate was measured in vitro at KCN concentrations shown to inhibit ciliary beat frequency. Pick-up rate was likewise inhibited by KCN and remained significantly depressed after washout of KCN. These data show that cyanide is a potent inhibitor of both ciliary beat frequency and oocyte cumulus complex pick-up rate and that its concentration in smoke solutions is sufficiently high to explain the previously reported inhibition of these oviductal processes.

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Section: Effect Of Cyanide On Occ Pick-up Rate (Opr) In Vitromentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Identification of Cigarette Smoke Components That Alter Functioning of Hamster (Mesocricetus auratus) Oviducts In Vitro1

Talbot¹,

DiCarlantonio

Knöll

et al. 1998

Biology of Reproduction

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“…OCCs are ovulated into the peritoneal cavity of humans and into the bursal cavity of rodents [1][2][3]. Once contact is made between an OCC and the infundibulum of the oviduct, the ciliated cells of the oviductal epithelium transfer the OCCs to the ampulla, where fertilization occurs [3][4][5]. Pick-up of OCCs by the oviductal infundibulum is the first in an important series of gamete transport events that assure entry of embryos into the uterus at the proper time.…”

Section: Introductionmentioning

confidence: 99%

Assay and Importance of Adhesive Interaction Between Hamster (Mesocricetus auratus) Oocyte-Cumulus Complexes and the Oviductal Epithelium1

Lam

Gieseke

Knöll

et al. 2000

Biology of Reproduction

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Adhesion between the oocyte-cumulus complex and infundibulum plays an important, but poorly understood, role in oocyte pick-up. The purposes of this study were to determine which components of the oocyte-cumulus complex and oviductal epithelium function in adhesion, to measure adhesion under physiological conditions, and to examine the effect of modulation of adhesion on oocyte-cumulus complex pick-up rate. Oocyte-cumulus complexes containing an expanded matrix were readily transported into the oviduct, while unexpanded complexes lacking an extracellular matrix were not picked up, indicating that the matrix is necessary for pick-up. Transmission electron microscopy revealed that during pick-up, adhesion occurred specifically between the ciliary crowns of the oviduct and the granules and filaments of the cumulus matrix. An assay was developed using vacuum from a low-flow peristaltic precision pump, modified for bi-directional flow, to measure the strength of adhesion between the oocyte-cumulus complex and the oviductal epithelium, and adhesion was measured during physiological conditions. The lectin wheat germ agglutinin and the polycation poly-L-lysine were then used to modulate adhesion, and the effects of increasing or decreasing adhesion on oocyte pick-up rate and ciliary beat frequency were examined. The data show that 1) the matrix of the oocyte-cumulus complex and the ciliary crowns of the oviduct function in adhesion during pick-up and that adhesion is necessary for pick-up, 2) adhesion can be assayed quantitatively and is very uniform among control infundibula, and 3) decreasing or increasing adhesion decreases oocyte pick-up rate and in some cases prevents pick-up without affecting ciliary beat frequency.

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In vitro assessment of reproductive toxicity of tobacco smoke and its constituents

Talbot

2008

Birth Defects Research Pt C

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Epidemiological studies have repeatedly shown that reproductive processes in pregnant women are adversely affected by exposure to cigarette smoke. The potential reproductive targets of smoke during pregnancy include the ovaries, oviducts, uterus, placenta, umbilical cord, and embryo/fetus. In vitro methods for studying the effects of smoke and its individual components have been developed and applied to each of these reproductive targets. In vitro assays have been useful in determining the biological processes that are affected in the reproductive organs and in identifying the cellular and molecular targets of smoke in each organ. In vitro methods have also been used to study the mechanism of action of smoke constituents, such as nicotine, on specific processes in reproductive organs and to screen smoke solutions to identify the molecules that affect reproduction. In general, data collected in vitro have confirmed, extended, and helped explain what has been learned from epidemiological studies. This review summarizes some of the in vitro assays that have been used to study cigarette smoke's effect on the nonpregnant and pregnant female reproductive tract and spotlights examples of their applications.

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In vitro analysis of oocyte cumulus complex pickup rate in the hamster Mesocricetus auratus

Cited by 21 publications

References 19 publications

Identification of Cigarette Smoke Components That Alter Functioning of Hamster (Mesocricetus auratus) Oviducts In Vitro1

Identification of Cigarette Smoke Components That Alter Functioning of Hamster (Mesocricetus auratus) Oviducts In Vitro1

Assay and Importance of Adhesive Interaction Between Hamster (Mesocricetus auratus) Oocyte-Cumulus Complexes and the Oviductal Epithelium1

In vitro assessment of reproductive toxicity of tobacco smoke and its constituents

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