In Vitro Analysis of Platelet Function During Storage of Platelets from Plasmapheresed Donors

Moore, Gerald L.; Mallin, William S.; Roberts, Stanley C.; Failla, Mark L.; Gray, John

doi:10.1111/j.1537-2995.1973.tb05458.x

Cited by 14 publications

(3 citation statements)

References 8 publications

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“…Other investigators [10,11] have reported measurements of PF3 in platelet concentrates (PC), but the assays employed were a test of the ability of platelets to respond to a challenge (e.g., incubation with kaolin) rather than a measurement of the PF3 activity already expressed in the sample. Also, the Stypven PF3 assay used by Champion [12] to study stored platelets is known to respond nonspecifically to neutral phospholipids and lipoproteins [13] , and, therefore, cannot be relied upon for comparison of PF3 levels between different concentrates.…”

Section: Introductionmentioning

confidence: 99%

Analysis of Platelet Factor 3 in Platelet Concentrates Stored for Transfusion

Bode¹,

Miller²

1986

Vox Sanguinis

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The amount of platelet factor 3 (PF3) activity expressed in stored platelet concentrates (PC) was measured in conjunction with extracellular LDH levels. Standard manual techniques for preparation of PC resulted in PF3 and LDH levels remarkably higher than those observed in PC prepared by apheresis or special manual plateletpheresis. During storage of PC, PF3 activity rose 2- to 10-fold, while LDH levels rose < 2-fold over starting values. Loss of LDH and appearance of PF3 expressed as a percent of total per platelet were significantly correlated only in standard, manual PC. Approximately half of the PF3 activity observed in any type of PC remained in the supernatant plasma after centrifugation. Upon gel filtration, the supernatant PF3 activity eluted in a high molecular weight peak containing phosphate and light-scattering material. Our findings indicate that platelets in standard, citrated PC express PF3 in amounts that approach that of frozen-thawed (lysed) platelets; however, the manner in which the PF3 activity appears suggests that stored platelets undergo a combination of activation and damage processes.

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Section: Introductionmentioning

confidence: 99%

Analysis of Platelet Factor 3 in Platelet Concentrates Stored for Transfusion

Bode¹,

Miller²

1986

Vox Sanguinis

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“…Several aspects of the in vitro function of platelets stored as platelet concentrates and destined for transfusion therapy have been studied in the past [1,9,14,27,29,37]. Platelets suspended and stored in their own plasma quickly lose the capacity of agonist-induced aggregation [24,28,36].…”

Section: Discussionmentioning

confidence: 99%

The evolution of platelet procoagulant activity of remnant platelets in stored platelet concentrates prepared by the platelet-rich plasma method and the buffy coat method

Planken

Vertessen

Mortelmans

et al. 1999

Annals of Hematology

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Platelets stored as concentrates are gradually activated (storage lesion), a process associated with changes in the expression of platelet procoagulant activity (PPCA). The aim of the present study was to evaluate the evolution of PPCA and the mean platelet volume (MPV) of stored platelets prepared according to the platelet-rich method (PRM) and the buffy coat method (BCM). Using the platelet factor 3 availability clotting test (PF3AT) on appropriately diluted concentrate samples, we found a decrease in PPCA expression of remnant platelets as a function of storage time (0.025 < p < 0.01 between day 1 and 7) in PRM-derived but not in BCM-derived platelet concentrates. Using the PF3AT reduction test we found a more important clotting time reduction in samples obtained from BCM than in samples obtained from PRM platelet concentrates, suggesting a higher PPCA expression of BCM platelets, not significant after 1 day but highly significant after 3 days (p < 0.0005) and after 7 days (p < 0.0005) of storage, as compared with PRM platelets. For both PRM and BCM concentrates there were no significant MPV changes as a function of storage time, but at any storage day the MPV of BCM concentrates was significantly higher (p < 0.0005) than the MPV of PRM concentrates. We conclude that the decrease of PPCA expression in PRM-derived concentrates as a function of storage time is in agreement with the gradual decrease of the platelet activation status in PRM concentrates during storage. There are probably several factors or variables causing platelets of BCM concentrates to express higher PPCA than those of PRM concentrates. Higher PPCA expression in BCM concentrates may be explained by an intrinsic platelet property, such as a difference in MPV between the two kinds of concentrates, or it may be related to an extrinsic factor such as different storage media, e.g., undiluted autologous plasma in PRM concentrates versus Plasmalyte A-diluted autologous plasma in BCM concentrates. Whether the difference in PPCA expression of remnant platelets in PRM and BCM concentrates is just an in vitro laboratory finding or may have consequences for the therapeutic efficiency of the concentrates is an interesting, still unresolved question.

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“…pH, p02 and pC02, aggregation and adhesivity of stored platelets have also been investigated in order to assess our working conditions. A simple and reliable in vitro test for predicting the posttransfusion survival and hemostatic effectiveness of preserved plate lets would be extremely useful in quality control and in assessing methods of platelet concentrate (PC) preservation.Widely used tests for platelet viability in clude the clot retraction, promoted by sen sibilized platelets [1], the photometric mea surement of ADP-induced aggregation [6,9,12], adhesivity to glass surfaces [4,6], the hypotonic shock response (HSR) [3,7,15], and serotonin uptake [5,10]. Among the methods listed, only serotonin uptake and HSR have been shown to correlate with 1 We are indebted to Prof. P. Lundsgaard-Hansen for invaluable help in performing statistical anal ysis and critical reading of the manuscript.…”

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confidence: 99%

An Evaluation of N-Ethyl-Maleimide-Induced Lipid Peroxidation in Platelet Concentrates Stored at 4 or 22°C

Valbonesi¹,

Garelli²,

Mosconi³

et al. 1979

Vox Sanguinis

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A method for the quality control of platelet viability, based on N-ethyl-maleimide (NEM)-induced lipid peroxidation, is reported. Duplicate platelet concentrates from individual donors were stored at 22 and 4°C, respectively. At time O and at 24-hour intervals, pH, O(2) and CO(2) tensions, ADP-induced aggregation, glass adhesivity, hypotonic shock response (HSR) and NEM-induced lipid peroxidation were measured. A highly significant correlation between NEM-induced lipid peroxidation and HSR was established, which suggests that the former method can be used for the in vitro prediction of posttransfusion survival of preserved platelets

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In Vitro Analysis of Platelet Function During Storage of Platelets from Plasmapheresed Donors

Cited by 14 publications

References 8 publications

Analysis of Platelet Factor 3 in Platelet Concentrates Stored for Transfusion

Analysis of Platelet Factor 3 in Platelet Concentrates Stored for Transfusion

The evolution of platelet procoagulant activity of remnant platelets in stored platelet concentrates prepared by the platelet-rich plasma method and the buffy coat method

An Evaluation of N-Ethyl-Maleimide-Induced Lipid Peroxidation in Platelet Concentrates Stored at 4 or 22°C

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