In vitro analysis of the role of the mitochondrial apoptosis pathway in CSBE therapy against human gastric cancer

Yu-bin, JI; Li, Yu

doi:10.3892/etm.2015.2779

Cited by 11 publications

(8 citation statements)

References 28 publications

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“…The expression of Bcl-2 family members, Bcl-2 (an anti-apoptotic protein) and Bax (a pro-apoptotic protein), are known to be associated with the apoptotic process. In addition, the imbalance between Bcl-2 and Bax results in the release of Cyt C from the mitochondria, which in turn activates the downstream caspases ( 38 , 39 ). Caspases include various cysteine proteases that are responsible for cell apoptosis in eukaryotes ( 40 ).…”

Section: Discussionmentioning

confidence: 99%

Allicin protects against H2O2-induced apoptosis of PC12 cells via the mitochondrial pathway

et al. 2017

Experimental and Therapeutic Medicine

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Allicin is a major bioactive ingredient of garlic and has a broad range of biological activities. Allicin has been reported to protect against cell apoptosis induced by H2O2 in human umbilical vein endothelial cells. The present study evaluated the neuroprotective effect of allicin on the H2O2-induced apoptosis of rat pheochromocytoma PC12 cells in vitro and explored the underlying mechanism involved. PC12 cells were incubated with increasing concentrations of allicin and the toxic effect of allicin was measured by MTT assay. The cells were pretreated for 24 h with low dose (L-), medium dose (M-) and high dose (H-) of allicin, followed by exposure to 200 µM H2O2 for 2 h, and the cell viability was examined by MTT assay. In addition, cell apoptosis rate was analyzed by Annexin V-FITC/PI assay, while intracellular reactive oxygen species (ROS) and mitochondrial transmembrane potential (∆ψm) were measured by flow cytometry. Bcl-2, Bax, cleaved-caspase-3 and cytochrome c (Cyt C) in the mitochondria were also examined by western blotting. The results demonstrated that 0.01 µg/ml (L-allicin), 0.1 µg/ml (M-allicin) and 1 µg/ml (H-allicin) were non-toxic doses of allicin. Furthermore, H2O2 reduced cell viability, promoted cell apoptosis, induced ROS production and decreased ∆ψm. However, allicin treatment reversed the effect of H2O2 in a dose-dependent manner. It was also observed that H2O2 exposure significantly decreased Bcl-2 and mitochondrial Cyt C, while it increased Bax and cleaved-caspase-3, which were attenuated by allicin pretreatment. The results revealed that allicin protected PC12 cells from H2O2-induced cell apoptosis via the mitochondrial pathway, suggesting the potential neuroprotective effect of allicin against neurological diseases.

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Section: Discussionmentioning

confidence: 99%

Allicin protects against H2O2-induced apoptosis of PC12 cells via the mitochondrial pathway

et al. 2017

Experimental and Therapeutic Medicine

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“…Analysis of cyto-morphological alterations in the apoptosis monitoring implies studying of apoptosis by standard light microscopy using histological dyes that enable distinguishing between apoptosis and necrosis. Using fluorescence dyes (Hoechst 33258, acridine orange, Annexin V-FITC/PI) contributes to extended range of fluorescence microscopy, flow cytometric and immunofluorescence assays in apoptosis detection (Ji & Yu, 2015;Zhuo et al, 2015). Analysis of apoptosis can also be conducted using electron microscopy that clearly reveals DNA condensation in the nucleus and other morphologic changes characteristic for apoptosis (McCarthy & Evan, 1998).…”

Section: In Vitro and In Vivo Methods For Apoptosis Researchmentioning

confidence: 99%

“…One of the useful methods for assessing apoptosis and monitoring drug response in hematological tumors is based on fluorodeoxyglucose-based positron emission tomography (FDG-PET) described by Newbold et al (2014). Analysis of apoptosis is also possible using rhodamine 123 fluorescence dye intensity to measure mitochondrial membrane potential via flow cytometry (Ji and Yu, 2015). In addition, DNA laddering technique is used to visualize the endonuclease cleavage products of apoptosis.…”

Section: In Vitro and In Vivo Methods For Apoptosis Researchmentioning

confidence: 99%

Triggering apoptosis in tumors: an overview of potential approaches in treatment of leukemia

Hadžić

Haverić

Pojskić

2019

GenApp

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Apoptosis, as a well-studied process of a programmed cell death, is essential for the maintenance of cell homeostasis and integrity of organisms. This process occurs normally during development and aging and it is a balance of the sustainability of the tissue cell population. In addition, apoptosis also occurs as a defensive mechanism such as an immune response or after cell damage as a consequence of a pathological condition or the action of harmful agents. Apoptotic activation tends to be less responsive with aging, causing accumulation of non-functional cells and pathological changes such are degenerative diseases or tumor transformation. This overview aims to provide summarized facts about different approaches of apoptosis research, targeting and regulation in tumors especially in leukemic cells as a way of pharmacological manipulation with a potential therapeutic benefit.

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“…Apoptosis analysis was performed as described previously ( 18 ). Briefly, MSCs were harvested from the cell culture flasks at approximately 80–90% confluence and centrifuged at 300 × g for 10 min at 4°C.…”

Section: Methodsmentioning

confidence: 99%

Role of microRNA 146a on the healing of cornea alkali burn treated with mesenchymal stem cells

Luo

Liang

et al. 2018

Mol Med Report

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The aim of the present study was to investigate the effect of microRNA 146a (miR146a) on promoting the repair of corneal alkali burn with bone marrow mesenchymal stem cells (MSCs). A total of 24 Sprague-Dawley female rats were divided into a normal group (Control), a normal MSC treatment group (Normal MSCs), an miR146a knockout MSC treatment group (miR146a-low MSCs) and an miR146a high-expression MSC treatment group (miR146a-high MSCs) according to the random number table. Quantitative polymerase chain reaction was used to evaluate the expression levels of miR146a. MTT assay was performed to measure the cell viability of mesenchymal stem cells (MSCs) and apoptosis was measured by flow cytometry. The expression levels of p65 nuclear factor (NF)-κB, proliferating cell nuclear antigen (PCNA) and Fas proteins were analyzed by western blotting. MSCs were tested for the secretion levels of vascular endothelial growth factor (VEGF), CD45, interferon (IFN)-γ and interleukin (IL)-10 by ELISA. The miR146a-high MSCs improved cell viability of MSCs and inhibited apoptosis of MSCs following alkali burn. miR146a-high MSCs decreased the expression levels of p65NF-κB and PCNA, and enhanced the expression level of Fas. Furthermore, miR146a-high MSCs improved the cornea opacity and enhanced the inhibition of neovascularization in the rats following alkali burn. miR146a-high MSCs inhibit the expression of VEGF, CD45, IFN-γ, while enhanced the expression of IL-10. Therefore, miR146a promotes the repair of corneal alkali burn in rats treated with MSCs.

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In vitro analysis of the role of the mitochondrial apoptosis pathway in CSBE therapy against human gastric cancer

Cited by 11 publications

References 28 publications

Allicin protects against H2O2-induced apoptosis of PC12 cells via the mitochondrial pathway

Allicin protects against H2O2-induced apoptosis of PC12 cells via the mitochondrial pathway

Triggering apoptosis in tumors: an overview of potential approaches in treatment of leukemia

Role of microRNA 146a on the healing of cornea alkali burn treated with mesenchymal stem cells

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