In Vitro and In Silico Screening Analysis of Artabotrys sumatranus Leaf and Twig Extracts for α-Glucosidase Inhibition Activity and Its Relationship with Antioxidant Activity

Rosa, Dela; Elya, Berna; Hanafi, Muhammad; Khatib, Alfi; Surya, Muhammad Imam

doi:10.3390/scipharm91010002

Cited by 6 publications

(5 citation statements)

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“…at a concentration of 7–8 µL mL −1 ( Gakuubi et al., 2017 ). Certain reports published the effect of flavonoids as an antifungal effect ( Orhan et al., 2010 ; Jin, 2019 ), and observed a flavonoid, a polyphenol compound (7, 9-Di-tert-butyl-1-oxaspiro (4, 5) deca-6, 9-diene-2, 8-dione) ( Rosa et al., 2022 ), in TMFE. The proposed mechanism of antifungal activity by use of MFE may be due to the inhibition of spore germination by preventing the emergence of a normal germ tube, leading to abnormal swelling and the branching of hyphae ( Paul et al., 2022 ).…”

Section: Resultsmentioning

confidence: 99%

Evaluation of phytoconstituents in marigold effluent for their antifungal activity against plant pathogens

Sanam,

Nagaraju,

P. S

et al. 2024

Front. Fungal Biol.

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The current study placed an intense emphasis on the excess discharge of agro-based industrial effluent and the use of plant extract antimicrobials to inhibit the growth of pathogens in crop plants. An effluent (treated and untreated) from the marigold flower processing industry has been identified for the presence of volatile and semi-volatile organic compounds, and a total of 18 in treated effluent and 23 in untreated effluent were found using gas chromatography–mass spectrometry. A total of 13 classes were identified, which include carboxylic acid, phenols, esters, alkanes, alkenes, alcohols, cyanide, heterocyclic, flavonoids, aldehydes, polycyclic aromatic, cycloalkanes, and cycloalkenes. A principal component analysis with varimax rotation was applied to discern the abundance of identified compounds under each class. An in vitro antifungal bioassay was conducted using effluents at three different concentrations against plant pathogens (Alternaria alter nata, Sclerotium rolfsii, Rhizoctonia solani, Pythium aphanidermata, Fusarium oxysporum, and Colletotrichum gloeosporioides). The study proved that treated and untreated effluents clearly inhibited the growth of fungal pathogens by 10 to 32% and 37 to 92%, respectively. The findings suggest that marigold flower effluent can be a promising resource for developing new plant protection methods that are effective against pathogenic fungi.

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Section: Resultsmentioning

confidence: 99%

Evaluation of phytoconstituents in marigold effluent for their antifungal activity against plant pathogens

Sanam,

Nagaraju,

P. S

et al. 2024

Front. Fungal Biol.

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“…Prophylactic activity, antioxidant, antibacterial, anti-diarrhoeal, anti-inflammatory, antihyperglycemic activity (Mathi et al, 2015;Senthil et al, 2016;Mohammed et al, 2016;Sahi 2016) γ-Tocopherol 0.54 Antineoplastic (Cooney et al, 1993, anticarcinogenesis (Takahashi et al, 2009, antiinflammatory (Reiter et al, 2007, antioxidant (Brigelius-Flohe et al, Antioxidant, anti-inflammatory (Choi et al, 2013;Nair et al, 2018), insecticidal and nematicidal (Chen et al, 2015), antibacterial (Aissaoui et al, 2019), antiviral (Leila et al, 2019) Anti-tumor (Takahashi et al,1995), antiinflammatory (Das and Himaja 2014), larvicidal effects (Sowmiya et al, 2017) Antimicrobial, antioxidant, anti-cancer, hypoglycaemic activities (Prajna et al, 2016;Sunita et al, 2017), anti-inflammatory and antiallergic properties (Zhang et al, 2019), αglucosidase inhibitors (Rosa et al, 2023).…”

Section: Results Results Resultsmentioning

confidence: 99%

Metabolite profiling of wild underutilized raspberry (Rubus pyrifolius)

ISMAINI,

SURYA

2023

Not Sci Biol

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Rubus pyrifolius J.E. Smith, one of the Rubus species, has been found in Indonesia and collected in the Cibodas Botanical Garden. Rubus species are known for their diverse triterpenes, each with unique biological functions. This research aimed to analyse the metabolite profiles of R. pyrifolius using n-hexane and methanol solvents. GC-MS analysis was used to identify volatile and semi-volatile compounds in the R. pyrifolius extracts, with compound identification based on NIST 17 through GC/MS mass spectra analysis. The results showed that the total number of identified phytochemical compounds in methanol and n-hexane extracts of R. pyrifolius were 115 and 174, respectively. Methanol extracts from R. pyrifolius flower, young leaf, mature leaf, and young stem exhibited 46, 20, 15, and 34 compounds, respectively. In contrast, n-hexane extracts from R. pyrifolius flower, young leaf, mature leaf, and young stem contained 35, 47, 29, and 63 compounds, respectively. These compounds were classified into seven phytochemical groups: alkanes, alkenes, cyclic ethers, diterpenes, fatty acids, triterpenes, and vitamin E. Furthermore, only carboxylic esters, ergosterols, esters, fatty alcohols, and phenols were found in n-hexane extracts. The methanol extract showed seven significant phytochemical groups, including linolenic acid, phthalate esters, phytol, phytosterol, sterol lipids, terpenoids, and triterpenoids. R. pyrifolius possesses a variety of bioactive phytochemical profiles that are relevant in the field of phytopharmaceuticals. Nevertheless, further research is essential to determine their biological activity.

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“…Ascorbic acid was used as the positive control solution. The analysis results were expressed as Fe 2+ mol/g extract and can be calculated using the following formula (Rosa et al, 2023):…”

Section: α-Glucosidase Inhibition Assaymentioning

confidence: 99%

“…The following formula (Rosa et al 2023) was used to calculated total TPC which was shown as milligram of gallic acid equivalent (GAE) per gram extract. GAE in ppm for sample was expressed as , and was the extract concentration in ppm.…”

Section: α-Glucosidase Inhibition Assaymentioning

confidence: 99%

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The Analysis of Artabotrys hexapetalus Stem Bark and Leaf Ethanol Extract as α-Glucosidase Inhibitor in Relation to Antioxidant Activity, Phenolic, and Flavonoid Contents Using In Vitro Analysis, LC-MS, Machine Learning and Molecular Docking

Rosa

Elya

Hanafi

et al. 2023

Preprint

Self Cite

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Artabotrys hexapetalus is one of Artabotrys species that can be found in Asia, such as Sri Lanka, India, China, Malaysia, Indonesia, and other Southeast Asian countries. This plant is used traditionally as a herbal medicine. The aims of this research were to explore the potential of A. hexapetalus leaf and stem bark ethanol extract to treat type 2 diabetes (T2D) by inhibiting the activity of α-glucosidase, including its relationship with the antioxidant activity, phenolic content, and flavonoid content. The analysis was done using α-glucosidase inhibition assay, DPPH assay, FRAP assay, TPC, TFC, UHPLC-QTOF-MS/MS, and molecular docking analysis. Results showed that stem bark extract had medium strong α-glucosidase inhibitory activity with IC50 value of 47.084 ppm, whereas the leaf extract had weak α-glucosidase inhibitory activity with IC50 value of 104.755 ppm. Random permutation in random forest simulation was used to predict the factors that contribute to the α-glucosidase inhibition. For stem bark, the α-glucosidase inhibition activity was influenced by antioxidant activity and phenolic compounds. Nevertheless, the main active compounds that play role in α-glucosidase inhibition in stem bark were likely from terpene groups. Meanwhile, the active compounds in the leaf extract were likely not antioxidants and did not belong to the phenolic and flavonoid group. Comparisons between various analysis were shown to corroborate the random permutation results.

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In Vitro and In Silico Screening Analysis of Artabotrys sumatranus Leaf and Twig Extracts for α-Glucosidase Inhibition Activity and Its Relationship with Antioxidant Activity

Cited by 6 publications

References 53 publications

Evaluation of phytoconstituents in marigold effluent for their antifungal activity against plant pathogens

Evaluation of phytoconstituents in marigold effluent for their antifungal activity against plant pathogens

Metabolite profiling of wild underutilized raspberry (Rubus pyrifolius)

The Analysis of Artabotrys hexapetalus Stem Bark and Leaf Ethanol Extract as α-Glucosidase Inhibitor in Relation to Antioxidant Activity, Phenolic, and Flavonoid Contents Using In Vitro Analysis, LC-MS, Machine Learning and Molecular Docking

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