In vitro and in silico analysis of ‘Taikong blue’ lavender essential oil in LPS-induced HaCaT cells and RAW264.7 murine macrophages

Wei, Mengya; Liu, Fei; Raka, Rifat Nowshin; Xiang, Jie; Xiao, Junsong; Han, Tae-Ho; Guo, Fu; Yang, Suzhen; Wu, Hua

doi:10.1186/s12906-022-03800-0

Cited by 7 publications

(4 citation statements)

References 38 publications

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“…These results indicate that bornyl acetate revealed the best pharmacokinetic properties compared to cubebol, and it can be considered in further experiments. Similar to our study, Wei et al, who found linalool and linalyl acetate (29.48 and 40.97%, respectively) in lavender, proved that these major LSEO components had no toxicity and were safe to be used as food or medication [ 78 ]. A recent study reported that linalyl acetate (39.7%), linalool (33.6%), and terpinen-4-ol (14.9%) were the most abundant lavender EOs and that they possessed antiviral activities against many DNA and RNA viruses [ 79 ].…”

Section: Resultssupporting

confidence: 91%

Chemical Profiling and Biological Properties of Essential Oils of Lavandula stoechas L. Collected from Three Moroccan Sites: In Vitro and In Silico Investigations

Benali

Lemhadri

Harboul

et al. 2023

Plants

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The aim of this study was the determination of the chemical compounds of Lavandula stoechas essential oil from Aknol (LSEOA), Khenifra (LSEOK), and Beni Mellal (LSEOB), and the in vitro investigation of their antibacterial, anticandidal, and antioxidant effects, and in silico anti-SARS-CoV-2 activity. The chemical profile of LSEO was determined using GC-MS-MS analysis, the results of which showed a qualitative and quantitative variation in the chemical composition of volatile compounds including L-fenchone, cubebol, camphor, bornyl acetate, and τ-muurolol; indicating that the biosynthesis of essential oils of Lavandula stoechas (LSEO) varied depending on the site of growth. The antioxidant activity was evaluated using the ABTS and FRAP methods, our results showed that this tested oil is endowed with an ABTS inhibitory effect and an important reducing power which varies between 4.82 ± 1.52 and 15.73 ± 3.26 mg EAA/g extract. The results of antibacterial activity of LSEOA, LSEOK and LSEOB, tested against Gram-positive and Gram-negative bacteria, revealed that B. subtilis (20.66 ± 1.15–25 ± 4.35 mm), P. mirabilis (18.66 ± 1.15–18.66 ± 1.15 mm), and P. aeruginosa (13.33 ± 1.15–19 ± 1.00 mm) are the most susceptible strains to LSEOA, LSEOK and LSEOB of which LSEOB exhibits bactericidal effect against P. mirabilis. furthermore The LSEO exhibited varying degrees of anticandidal activity with an inhibition zones of 25.33 ± 0.5, 22.66 ± 2.51, and 19 ± 1 mm for LSEOK, LSEOB, and LSEOA, respectively. Additionally, the in silico molecular docking process, performed using Chimera Vina and Surflex-Dock programs, indicated that LSEO could inhibit SARS-CoV-2. These important biological properties of LSEO qualify this plant as an interesting source of natural bioactive compounds with medicinal actions.

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Section: Resultssupporting

confidence: 91%

Chemical Profiling and Biological Properties of Essential Oils of Lavandula stoechas L. Collected from Three Moroccan Sites: In Vitro and In Silico Investigations

Benali

Lemhadri

Harboul

et al. 2023

Plants

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“…A dexamethasone group (DEX, 1 μM) was used as a positive control. After induction with 1 μg/mL LPS for 4 h, RNA was extracted, and cDNA was prepared using real-time fluorescence quantitative PCR according to the method from Vazyme [ 88 ]. The primers were synthesized by Sangon Biotech (Shanghai) Co., Ltd. (Shanghai, China), and the sequences are shown in the Supplementary Material (Table S1) .…”

Section: Methodsmentioning

confidence: 99%

Study of the Structure and Bioactivity of Polysaccharides from Different Parts of Stemona tuberosa Lour

Qiu,

Ou,

et al. 2024

Molecules

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The polysaccharides from Stemona tuberosa Lour, a kind of plant used in Chinese herbal medicine, have various pharmacological activities, such as anti-inflammatory and antioxidant properties. However, the effects of the extraction methods and the activity of polysaccharides from different parts are still unknown. Therefore, this study aimed to evaluate the effects of different extraction methods on the yields, chemical compositions, and bioactivity of polysaccharides extracted from different parts of Stemona tuberosa Lour. Six polysaccharides were extracted from the leaves, roots, and stems of Stemona tuberosa Lour through the use of hot water (i.e., SPS-L1, SPS-R1, and SPS-S1) and an ultrasound-assisted method (i.e., SPS-L2, SPS-R2, and SPS-S2). The results showed that the physicochemical properties, structural properties, and biological activity of the polysaccharides varied with the extraction methods and parts. SPS-R1 and SPS-R2 had higher extraction yields and total sugar contents than those of the other SPSs (SPS-L1, SPS-L2, SPS-S1, and SPS-S2). SPS-L1 had favorable antioxidant activity and the ability to downregulate MUC5AC expression. An investigation of the anti-inflammatory properties showed that SPS-R1 and SPS-R2 had greater anti-inflammatory activities, while SPS-R2 demonstrated the strongest anti-inflammatory potential. The results of this study indicated that SPS-L1 and SPS-L2, which were extracted from non-medicinal parts, may serve as potent natural antioxidants, but further study is necessary to explore their potential applications in the treatment of diseases. The positive anti-inflammatory effects of SPS-R1 and SPS-R2 in the roots may be further exploited in drugs for the treatment of inflammation.

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“…The HaCaT cells were cultured in DMEM supplemented with 10% FBS, 1% P/S and 1% glutamine and maintained in a humidified 5% CO 2 incubator at 37 °C. The cytotoxic activities of samples were measured by colorimetric MTT assay [ 20 ]. The cells were seeded into a 96-well plate at a density of 2 × 10 5 cells/mL.…”

Section: Methodsmentioning

confidence: 99%

Antibacterial activity of oregano essential oils against Streptococcus mutans in vitro and analysis of active components

Yuan

Sun

Song

et al. 2023

BMC Complement Med Ther

Self Cite

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Background Streptococcus mutans (S. mutans) is considered the most relevant bacteria during the transition of the non-pathogenic commensal oral microbial community to plaque biofilms that promote the development of dental caries. Oregano (Origanum vulgare L.), is a universally natural flavoring and its essential oil has been demonstrated to have good antibacterial effects. However, the specific antibacterial mechanism of oregano essential oil (OEO) against S. mutans is still not completely understood. Methods In this work, the composition of two different OEOs was determined by GC‒MS. Disk-diffusion method, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined to assess their antimicrobial effect on S. mutans. The inhibition of acid production, hydrophobicity, biofilm formation and real-time PCR for gtfB/C/D, spaP, gbpB, vicR, relA and brpA mRNA expression by S. mutans were assessed to preliminarily investigate the mechanisms of action. Molecular docking was performed to simulate the interactions with the virulence proteins and active constituents. MTT test using immortalized human keratinocytes cells was also performed to investigate cytotoxicity. Results Compared with the positive drug Penicillin /streptomycin 100X (DIZ: 34.13 ± 0.85 mm, MIC: 0.78125 μL/mL, MBC: 6.25 μL/mL), the essential oils of Origanum vulgare L. (DIZ: 80 mm, MIC: 0.625μL/mL, MBC:2.5μL/mL) and Origanum heracleoticum L. (DIZ: 39.67 ± 0.81 mm, MIC: 0.625μL/mL, MBC: 1.25μL/mL) could also exhibit similar effects to inhibit the acid production and reduce the hydrophobicity and biofilm formation of S. mutans at 1/2-1MIC concentration. And gene expression of gtfB/C/D, spaP, gbpB, vicR and relA were found to be downregulated. Due to the composition of essential oils from different sources being highly variable, through effective network pharmacology analysis, we found that OEOs contained many effective compounds, like carvacrol and its biosynthetic precursors γ-terpinene and p-cymene, which may directly target several virulence proteins of S. mutans. Besides, no toxic effect was instigated by OEOs at 0.1 μL/mL in the immortalized human keratinocytes cells. Conclusion The integrated analysis in the present study suggested that OEO might be a potential antibacterial agent for the prevention of dental caries.

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In vitro and in silico analysis of ‘Taikong blue’ lavender essential oil in LPS-induced HaCaT cells and RAW264.7 murine macrophages

Cited by 7 publications

References 38 publications

Chemical Profiling and Biological Properties of Essential Oils of Lavandula stoechas L. Collected from Three Moroccan Sites: In Vitro and In Silico Investigations

Chemical Profiling and Biological Properties of Essential Oils of Lavandula stoechas L. Collected from Three Moroccan Sites: In Vitro and In Silico Investigations

Study of the Structure and Bioactivity of Polysaccharides from Different Parts of Stemona tuberosa Lour

Antibacterial activity of oregano essential oils against Streptococcus mutans in vitro and analysis of active components

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