In vitro and in silico evaluations of resin-based dental restorative material toxicity

Bandarra, Susana; Mascarenhas, Paulo; Luís, Ana R; Catrau, Mafalda; Bekman, Evguenia; Ribeiro, Ana Clara; Félix, Sergio; Caldeira, Jorge; Barahona, Isabel

doi:10.1007/s00784-019-03131-4

Cited by 23 publications

(14 citation statements)

References 53 publications

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“…The consequences of both the manufacturing and polymerization processes, as well as the resin’s biodegradation, include various adverse effects on the oral health (irritation, inflammation, and allergic responses of the oral cavity tissues) due mainly to the release of potential cytotoxic components from the polymer network. Observations similar to the above-presented findings are also contained in the relatively recent study of Bandarra et al (2020) [ 48 ], which evaluated the cytocompatibility and the neurotoxic potential of the monomers for three interim conventional restoration materials, (Tab 2000, Kerr, USA (methyl methacrylate based), ProTemp 4, 3M, USA (bis-acrylic based), and Structur 3, Voco GmbH, Germany (urethane dimethacrylate/UDMA based)). The results of this study suggest that urethane dimethacrylate–based resin, even at low concentrations, may cause adverse local (oral) side effects and may have neurotoxic potential.…”

Section: Discussionsupporting

confidence: 76%

Biochemical Interaction between Materials Used for Interim Prosthetic Restorations and Saliva

et al. 2021

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The purpose of this study was to analyze the oxidative stress level and inflammatory status of saliva in the presence of certain materials used for obtaining interim prosthetic restorations. Four types of interim resin materials were investigated: a pressure/heat-cured acrylic resin (Superpont C+B, SpofaDental a.s Czech Republic, /KaVo Kerr Group), a milled resin (Telio CAD polymethyl methacrylate, Ivoclar Vivadent AG, Liechtenstein), a 3D printed resin (NextDent C&B MFH, NextDent by 3D Systems, the Netherlands), and a pressure/heat-cured micro-filled indirect composite resin (SR Chromasit, Ivoclar Vivadent AG, Liechtenstein). The disk-shaped resin samples (30 mm diameter, 2 mm high) were obtained in line with the producers’ recommendations. The resulting resin specimens were incubated with saliva samples collected from twenty healthy volunteers. In order to analyze the antioxidant activity of the tested materials, certain salivary parameters were evaluated before and after incubation: uric acid, gamma glutamyl transferase (GGT), oxidative stress responsive kinase-1 (OXSR-1), and total antioxidant capacity (TAC); the salivary levels of tumor necrosis factor (TNFα) and interleukin-6 (IL-6) (inflammatory markers) were measured as well. The obtained results are overall favorable, showing that the tested materials did not cause significant changes in the salivary oxidative stress level and did not influence the inflammatory salivary status.

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Section: Discussionsupporting

confidence: 76%

Biochemical Interaction between Materials Used for Interim Prosthetic Restorations and Saliva

et al. 2021

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“…The cytotoxicity assay evaluated the overall effect of the biomaterials on normal, human cell lines in vitro. Human dermal fibroblasts, as well as mouse 3T3 fibroblasts [37] or L929 fibroblasts [38], were previously employed for dental materials testing [39,40,41], since the cytotoxic effect is similar to that exerted on oral fibroblasts, while the HUVECs [42] were used for comparison, because they are fetal cells that can mimic the stem cells behavior when exposed to eluted substances from the tested biomaterials.…”

Section: Methodsmentioning

confidence: 99%

New Pre-reacted Glass Containing Dental Composites (giomers) with Improved Fluoride Release and Biocompatibility

et al. 2019

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The aim of the present work was to prepare a series of novel restorative giomers and investigate the morphology, the physico-chemical properties (residual monomer, fluoride release), and the cytotoxicity of the new materials. The experimental giomers were prepared as light-cured pastes by blending different resin matrices comprising aromatic/aliphatic/urethane (di) methacrylates, with hybrid fillers containing pre-reacted glasses (PRGs), a radiopaque glass, and nano fluorhydroxyapatite. Polyalkenoic acids based on acrylic acid/itaconic acid/N-acryloyl -L-leucine modified or not with methacrylic groups, together with a superficially active glass, were used to prepare the PRGs. The fluoride ion release of the experimental giomers was investigated within a period of 60 days of storage in bidistilled water while using a fluoride ion selective electrode. Beautifil II commercial product was used as a reference. Cell cytotoxicity tests were done in vitro, in accordance with ISO 10993-122012 proceedings. Human dermal fibroblasts and umbilical endothelial vein cultures were used. The values that were obtained for cumulative fluoride release for all experimental giomers were higher than for the Beautifil II product, being more than twice the ones that were obtained for the commercial product after 60 days of storage in bidistilled water. The experimental biomaterials showed similar and/or better results when compared to the commercial one; this effect was maintained in all tested conditions.

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“…NIH/3T3 (Swiss mouse embryo) fibroblasts (93061524, Sigma-Aldrich, Saint Louis, MO, USA) were sub-cultured in Dulbecco's Modified Eagle's Medium (DMEM, Sigma-Aldrich, Saint Louis, MO, USA) supplemented with 10% of fetal bovine serum (FBS, Sigma-Aldrich, Saint Louis, MO, USA) and 1% penicillin-streptomycin solution (10,000 U•mL −1 of penicillin and 10 mg•mL −1 of streptomycin, Sigma-Aldrich, Saint Louis, MO, USA) using T75 flasks with filter caps (Thermo Fisher Scientific, Kandel, Germany). The cells were grown in a humidified incubator at 37 • C in air containing 5% CO 2 , as described elsewhere [47]. Morphological examinations of cells were performed daily using an inverted light microscope (Axiovert ® 25, ZEISS Microscopy, Jena, Germany).…”

Section: Cytotoxicity Evaluationmentioning

confidence: 99%

Tough and Low Friction Polyvinyl Alcohol Hydrogels Loaded with Anti-inflammatories for Cartilage Replacement

et al. 2020

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The development of new materials that mimic cartilage and its function is an unmet need that will allow replacing the damaged parts of the joints, instead of the whole joint. Polyvinyl alcohol (PVA) hydrogels have raised special interest for this application due to their biocompatibility, high swelling capacity and chemical stability. In this work, the effect of post-processing treatments (annealing, high hydrostatic pressure (HHP) and gamma-radiation) on the performance of PVA gels obtained by cast-drying was investigated and, their ability to be used as delivery vehicles of the anti-inflammatories diclofenac or ketorolac was evaluated. HHP damaged the hydrogels, breaking some bonds in the polymeric matrix, and therefore led to poor mechanical and tribological properties. The remaining treatments, in general, improved the performance of the materials, increasing their crystallinity. Annealing at 150 °C generated the best mechanical and tribological results: higher resistance to compressive and tensile loads, lower friction coefficients and ability to support higher loads in sliding movement. This material was loaded with the anti-inflammatories, both without and with vitamin E (Vit.E) or Vit.E + cetalkonium chloride (CKC). Vit.E + CKC helped to control the release of the drugs which occurred in 24 h. The material did not induce irritability or cytotoxicity and, therefore, shows high potential to be used in cartilage replacement with a therapeutic effect in the immediate postoperative period.

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In vitro and in silico evaluations of resin-based dental restorative material toxicity

Cited by 23 publications

References 53 publications

Biochemical Interaction between Materials Used for Interim Prosthetic Restorations and Saliva

Biochemical Interaction between Materials Used for Interim Prosthetic Restorations and Saliva

New Pre-reacted Glass Containing Dental Composites (giomers) with Improved Fluoride Release and Biocompatibility

Tough and Low Friction Polyvinyl Alcohol Hydrogels Loaded with Anti-inflammatories for Cartilage Replacement

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