In Vitro and In Vivo Evaluation of Antitumor Activity of Ligustrum robustum, A Chinese Herbal Tea

Zuo, Haojiang; Liu, Shan; Yan, Chun; Li, Li-Man; Pei, Xiaofang

doi:10.1007/s11655-018-2983-5

Cited by 16 publications

(12 citation statements)

References 14 publications

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“…To preparation of LR extract for component analysis, 50 g LR was ground to a fine powder and suspended in 1500 mL distilled water and incubated with shaking at 80°C for 3 h. Then the extract was filtered and further evaporated on a rotary evaporator to a concentration to 1 g/mL and stored at −20°C until further use [10]. The analytical solution was prepared by filtering 220 μ L supernatant using a 0.22 μ m filter.…”

Section: Methodsmentioning

confidence: 99%

“…Ligustrum robustum ( LR ), a traditional Chinese beverage plant, contains abundant bioactive compounds with health benefits, such as flavonoids, total phenolic acid, polysaccharide, and triterpenoid [6, 7]. Animal studies have shown the antiobesity, antioxidative, anti-inflammatory, antitumor, and hepatoprotective effects of LR [8–10]. Moreover, the LR -induced changes in the composition of gut microbiota were also observed accompanied with weight loss in an animal study [11].…”

Section: Introductionmentioning

confidence: 99%

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Ligustrum robustum Intake, Weight Loss, and Gut Microbiota: An Intervention Trial

Zhou

Chen

et al. 2019

Evidence-Based Complementary and Alternative Medicine

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Ligustrum robustum (LR) shows antiobesity effects in animal studies. However, little is known about the effect on human. The present study aimed to investigate the effect of LR intake on weight change in obese women and the role of gut microbiota. Thirty overweight and obese female participants (BMI ≥24 kg/m2) were recruited in the current study. The participants drank LR 10g/d for 12 wks. Their body composition and related biomarkers were assessed. Alterations of the gut microbiota were analyzed using 16S rRNA sequencing. The primary outcome was the change in body weight. LR intake resulted in 2.5% weight loss over 12 wks (P<0.01). Change in body fat at 12 wk was -1.77 ± 1.19 kg (P<0.01). In addition, decreased Firmicutes-to-Bacteroidetes ratio (P=0.03), increased richness (the ACE estimator, P<0.01; the Chao1 estimator, P<0.01), and altered representative taxa of the gut microbiota were observed. Bacteroidaceae, Bacteroides, Bacilli, and Lactobacillales were higher while Ruminococcaceae, Enterobacteriaceae, Enterobacteriales, Lachnospiraceae, Clostridia, and Clostridiales were lower at 12 wk. Moreover, LR intervention decreased fasting glucose (P<0.01), serum leptin (P<0.01), and IL8 (P=0.02) and increased HOMA-β (P<0.01). LR intervention moderately decreased the body weight in overweight and obese women and such effect might be due to modulation of gut microbiota.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Ligustrum robustum Intake, Weight Loss, and Gut Microbiota: An Intervention Trial

Zhou

Chen

et al. 2019

Evidence-Based Complementary and Alternative Medicine

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“…It was widely demonstrated that the extract of this tea exhibits antioxidant activity and eliminates free radicals in food systems (Yu & Zeng, 2013). Moreover, it also exhibits anti‐inflammatory, antitumor, neuroprotective, and hepatoprotective activities (Chakraborty et al., 2012; Zuo et al., 2019). However, the research focus on the antimicrobial and anticaries effects of L. robustum is limited.…”

Section: Introductionmentioning

confidence: 99%

Activity of Ligustrum robustum (Roxb.) Blume extract against the biofilm formation and exopolysaccharide synthesis of Streptococcus mutans

Zhong

Zeng

Zhou

et al. 2020

Molecular Oral Microbiology

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Ligustrum robustum (Roxb.) Blume is utilized as a traditional Chinese herbal tea with various health benefits and protective effects. Streptococcus mutans is an important cariogenic oral bacteria species. The present study aimed to assess the influence of Ligustrum robustum extract (LRE) on the biofilm formation of S. mutans and the mechanism of its action, as well as to identify its chemical components. For chemical identification, HPLC‐MS and nuclear magnetic resonance were applied and four identified phytochemicals were reported (Ligurobustoside B, Ligurobustoside N, Ligurobustoside J, and Ligurobustoside C). The dose‐dependent (0.5 to 2.0 μg/μL) antimicrobial toxicity of LRE against S. mutans biofilm formation and exopolysaccharide (EPS) synthesis was evaluated by confocal laser scanning microscopy (CLSM), Crystal violet stain, and CFU counting. The microstructure of S. mutans biofilm treated with LRE was investigated both on glass coverslips and ex vivo bovine dental enamel by scanning electron microscopy (SEM). Moreover, LRE downregulated the expression of S. mutans glucosyltransferase‐encoding genes gtfB, gtfC, and gtfD, and the quorum sensing (QS) factors comD and comE, suggesting its toxic mechanism. In addition, the result of CCK‐8 test on human oral cells revealed an acceptable biocompatibility of LRE. These findings indicated the possible application of this daily consumed herbal tea for caries prevention.

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“…In 2016, around 1.9 billion grown-ups were overweight and just about 33% were stout [13]. Ku-Ding-Cha, truly harsh spike-leaf tea, is a sort of well-known home-grown tea generally utilized in China from numerous hundred years prior [14]. They are commonly treated with a wide assortment of antifungal medications [11].…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial Effect of Non-polar Compounds Extracted from Medicinal Plants

Ghazanfar¹,

Shahid²,

Liu³

et al. 2020

AJFN

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The learning is too focussing on identification of compounds dependable for the antimicrobial. Medicinal plants have a significant wellspring of pharmacological impacts that goes about as new of diseases, anti-infections, antioxidant and hostile to malignancy operators. In this study, antimicrobial and antioxidant properties of thyme (Thymus vulgaris L.) black pepper (Piper nigrum L.) Cinnamon (Cinnamomum verum L.) green tea (Camellia sinensis L.) nutmegs (Myristicafragrans Houtt) were investigated. Medicinal plants are generally devoured around the world; green tea contains a lot of non-oxidized flavonoids, named catechins. Therapeutic plants are additionally proof that lessens oxidative pressure hostile to diabetic and switches endothelial brokenness. The examination was led to investigate the antimicrobial and cancer prevention agent properties. The antimicrobial activities were analyzed by disc diffusion test against chosen microbial strain. Anti-microbial activity of n-hexane extract from medicinal plants against two "Gram positive" microscopic organisms viz Staphylococcus aureus and Bacillus subtils two "Gram negative" microorganisms, for example, Pasturellamultocida and Escherichia coli was performed. The antimicrobial effects showed that 30% concentration of n-hexane black pepper (Piper nigrum L.) extract had a strong activity against Bacillus subtills (64±1) and 50% concentration of thyme (Thymus vulgaris L.) extract against Staphylococcus aureus (23±1). The consequences of against oxidant activities uncovered that the most noteworthy all out phenolic substance and complete flavonoid substance were noted after extract of plants. Free radical rummaging action was most extreme in n-hexane extract of plants. Reducing power assay was most extreme extracts of plants. Biofilm activity of plants extracts n-hexane against two bacteria viz Staphylococcus aureus and such as Escherichia coli was done. The extract shows maximum hemolysis of erythrocytes. It was seen that all verified microbial strains were sensitive ">0.82 activity value" to extract that showed a higher antimicrobial impact. Keep in outlook the significance of flavonoids and other bioactive compounds this investigation concentrated on evaluating natural compounds utilizing remove at various fixation.

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In Vitro and In Vivo Evaluation of Antitumor Activity of Ligustrum robustum, A Chinese Herbal Tea

Cited by 16 publications

References 14 publications

Ligustrum robustum Intake, Weight Loss, and Gut Microbiota: An Intervention Trial

Ligustrum robustum Intake, Weight Loss, and Gut Microbiota: An Intervention Trial

Activity of Ligustrum robustum (Roxb.) Blume extract against the biofilm formation and exopolysaccharide synthesis of Streptococcus mutans

Antimicrobial Effect of Non-polar Compounds Extracted from Medicinal Plants

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