In vitro and in vivo acute response towards injectable thermosensitive chitosan/TEMPO-oxidized cellulose nanofiber hydrogel

Nguyen, Trang Ho Minh; Abueva, Celine; Ho, Hai Van; Lee, Sun-Young; Lee, Byong‐Taek

doi:10.1016/j.carbpol.2017.10.032

Cited by 73 publications

(39 citation statements)

References 32 publications

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“…Thermosensitive hydrogel based on CS and beta-glycerophosphate was developed, however, it presented some biocompatibility issues due to an increased amount of substances required for gelation at body temperature. Recently, it was shown that the addition of TEMPO-oxidized cellulose nanofiber induced faster gelation and increased porosity with improved biocompatibility in vitro and in vivo in comparison to CS [71]. CS could be layered on top of metal (e.g., titanium) implants to increase osteointegration [72,73].…”

Section: Bonementioning

confidence: 99%

Progress in the Development of Chitosan-Based Biomaterials for Tissue Engineering and Regenerative Medicine

et al. 2019

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Over the last few decades, chitosan has become a good candidate for tissue engineering applications. Derived from chitin, chitosan is a unique natural polysaccharide with outstanding properties in line with excellent biodegradability, biocompatibility, and antimicrobial activity. Due to the presence of free amine groups in its backbone chain, chitosan could be further chemically modified to possess additional functional properties useful for the development of different biomaterials in regenerative medicine. In the current review, we will highlight the progress made in the development of chitosan-containing bioscaffolds, such as gels, sponges, films, and fibers, and their possible applications in tissue repair and regeneration, as well as the use of chitosan as a component for drug delivery applications.

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Section: Bonementioning

confidence: 99%

Progress in the Development of Chitosan-Based Biomaterials for Tissue Engineering and Regenerative Medicine

et al. 2019

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“…Beyond this concentration (0.09 wt% CNFs), the trend was reversed, i.e., the G′ drop was slower and the critical strain for selfhealing increased. Because of these nonmonotonic changes, we hypothesized that apart from the Schiff-base dynamic bonding of DF-PEG, there may be additional interactions in this system such as the electrostatic interaction between the positive charge on the glycol CS and the negative charge of CNFs added 43,44 . The ionic network as well as the fibrous structure of CNFs 45,46 may have caused steric hindrance effects while Schiff-base bonds formed and undeformed, leading to changes in selfhealing.…”

Section: Discussionmentioning

confidence: 99%

Novel chitosan–cellulose nanofiber self-healing hydrogels to correlate self-healing properties of hydrogels with neural regeneration effects

et al. 2019

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Biodegradable self-healing hydrogels are attractive materials for tissue repair; however, the impact of the self-healing abilities of hydrogels on tissue repair is not clear. In this study, we prepared novel chitosan-cellulose nanofiber (CS-CNF) composite self-healing hydrogels with the same modulus (approximately 2 kPa) but tunable self-healing properties. By adding a low amount of CNFs (0.06-0.15 wt%) in the pristine chitosan (CS) self-healing hydrogel, the reversible dynamic Schiff bonding, strain sensitivity, and self-healing of the hydrogel are obviously affected. Neural stem cells embedded in the CS-CNF hydrogel with better self-healing properties reveal significantly enhanced oxygen metabolism as well as neural differentiation. The differentiation of neural stem cells is highly correlated with their metabolic change in the self-healing hydrogel. Moreover, the neural regeneration effect of the optimized CS-CNF hydrogel with 0.09 wt% CNFs and the best self-healing properties show a 50% improvement over the pristine CS hydrogel in the zebrafish brain injury model. A mechanism is proposed to interpret the tunable self-healing properties of CS-CNF hydrogels with stiffness maintained in a similar range. The new self-healing hydrogels help to clarify the role of self-healing in the biological performance of hydrogels as well as provide design rationale for hydrogels with better injectability and tissue regeneration potential.

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“…Inflammation provoked by hydrogel implants is determined by the type of biomaterial (Chen, Lianga, & Thouas, ), the chemical composition of the polymer (Barbosa et al, ; Vasconcelos et al, ) or the component composition of the hydrogel (Nguyen, Abueva, Ho, Lee, & Lee, ). We observed that pectic hydrogel implantation stimulated an acute phase of inflammation.…”

Section: Discussionmentioning

confidence: 99%

Mechanical properties of the pectin hydrogels and inflammation response to their subcutaneous implantation

Markov

Khramova

Shumikhin

et al. 2019

J Biomedical Materials Res

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We studied the influence of the mechanical properties of pectin hydrogels on acute inflammation and tissue repair after subcutaneous implantation. We used hard and soft pectin hydrogels. The results of histology and the analysis of serum‐level cytokines demonstrated that the intensity of acute inflammation increased with increasing hardness of the pectin hydrogels. We also showed that the pectin hydrogels did not inhibit tissue repair. The results of the morphometric and texture analysis of the pectin hydrogels showed that the in vivo biodegradation kinetics of hard hydrogels were greater than those of soft pectin hydrogels. We also observed that on the surface of the hard and soft pectin hydrogels, a network of collagen fibers was formed. The surface of the pectin hydrogel was shown to prevent the adhesion of infiltrating inflammatory cells. The results of the in vitro experiments demonstrated that pectin hydrogels inhibited the functional activity of macrophages and minimally activated the complement system. Therefore, we showed that soft pectin hydrogels have low proinflammatory potential and can be used in surgery as a barrier material as prevention of adhesions in the abdominal cavity. The hard pectin hydrogel can be used in tissue engineering. The hard pectin hydrogels can be used in the reconstruction of skin because are overpopulated with collagen fibers and contribute to the formation of new connective tissue, their elasticity is comparable to the skin and can be adjusted. They are biodegradable, and no additional manipulation is required to remove them.

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In vitro and in vivo acute response towards injectable thermosensitive chitosan/TEMPO-oxidized cellulose nanofiber hydrogel

Cited by 73 publications

References 32 publications

Progress in the Development of Chitosan-Based Biomaterials for Tissue Engineering and Regenerative Medicine

Progress in the Development of Chitosan-Based Biomaterials for Tissue Engineering and Regenerative Medicine

Novel chitosan–cellulose nanofiber self-healing hydrogels to correlate self-healing properties of hydrogels with neural regeneration effects

Mechanical properties of the pectin hydrogels and inflammation response to their subcutaneous implantation

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