In Vitro and In Vivo Effect of Peptides Derived from 14-3-3 Paracoccidioides spp. Protein

Scorzoni, Liliana; Silva, Ana Carolina Alves de Paula e; Oliveira, Haroldo César de; Santos, Catarina; Singulani, Júnya de Lacorte; Assato, Patrícia Akemi; Marcos, Caroline Maria; Oliveira, Lariane Teodoro; Fregonezi, Nathália Ferreira; Rossi, Diego Conrado Pereira; Silva, Leandro Buffoni Roque da; Taborda, Carlos Pelleschi; Fusco‐Almeida, Ana Marisa; Mendes‐Giannini, Maria José Soares

doi:10.3390/jof7010052

Cited by 6 publications

(6 citation statements)

References 75 publications

(100 reference statements)

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“…[61,65,75,76,80,100,102], Aspergillus fumigatus [84], Saccharomyces cerevisiae [60,82,89], Cryptococcus neoformans [79,90], and Candida albicans [83]; as well as in studies involving the development of vaccines for Candida albicans [77,78] and Paracoccidioides spp. [103]. Among these proteins, we have fructose-bisphosphate aldolase and glucan 1,3-beta-glucosidase, which are not related to homolog proteins in the human host [104].…”

Section: Discussionmentioning

confidence: 97%

Prospecting Biomarkers for Diagnostic and Therapeutic Approaches in Pythiosis

Chechi

Rotchanapreeda

Paz

et al. 2021

JoF

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Pythiosis, whose etiological agent is the oomycete Pythium insidiosum, is a life-threatening disease that occurs mainly in tropical and subtropical countries, affecting several animal species. It is frequently found in horses in Brazil and humans in Thailand. The disease is difficult to diagnose because the pathogen’s hyphae are often misdiagnosed as mucoromycete fungi in histological sections. Additionally, there is no specific antigen to use for rapid diagnosis, the availability of which could improve the prognosis in different animal species. In this scenario, we investigated which P. insidiosum antigens are recognized by circulating antibodies in horses and humans with pythiosis from Brazil and Thailand, respectively, using 2D immunoblotting followed by mass spectrometry for the identification of antigens. We identified 23 protein spots, 14 recognized by pooled serum from horses and humans. Seven antigens were commonly recognized by both species, such as the heat-shock cognate 70 KDa protein, the heat-shock 70 KDa protein, glucan 1,3-beta-glucosidase, fructose-bisphosphate aldolase, serine/threonine-protein phosphatase, aconitate hydratase, and 14-3-3 protein epsilon. These results demonstrate that there are common antigens recognized by the immune responses of horses and humans, and these antigens may be studied as biomarkers for improving diagnosis and treatment.

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Section: Discussionmentioning

confidence: 97%

Prospecting Biomarkers for Diagnostic and Therapeutic Approaches in Pythiosis

Chechi

Rotchanapreeda

Paz

et al. 2021

JoF

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“…It is supported by the Selvaggini et al [20] demonstration that augmented fungal hypersensitivity to Calcofluor is closely associated with high chitin content in the cell wall. Then we suggest that yeasts that overexpressed PCN, probably through increased chitinolytic activity, displayed cell wall weakening, an alteration that activates compensatory mechanisms to increase chitin In the last decade, G. mellonella larvae became a convenient host model for experimental PCM [14,[21][22][23][24][25], which attends to ethical and economic requirements for good animal experimentation practices. Then, we used this larval model to compare host damages caused by inoculation of wild-type yeasts and PCN-transformed yeasts.…”

Section: Discussionmentioning

confidence: 99%

“…In the last decade, G. mellonella larvae became a convenient host model for experimental PCM [14, 21-25], which attends to ethical and economic requirements for good animal experimentation practices. Then, we used this larval model to compare host damages caused by inoculation of wild-type yeasts and PCN-transformed yeasts.…”

Section: Discussionmentioning

confidence: 99%

Paracoccin impacts on Paracoccidioides brasiliensis virulence and susceptibility to antifungal drugs by modifying the expression of genes related to remodeling of the yeasts cell wall

Pitangui¹,

Fernandes²,

Silva³

et al. 2022

Preprint

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The study of the paracoccin lectin (PCN) has provided knowledge about its role in the biology of Paracoccidioides brasiliensis and in the pathogenesis of paracoccidioidomycosis (PCM). In this context, PCN has proved to be a promising immunomodulatory agent for the exploration of vaccine target molecules and/or for diagnostic or therapeutic purposes. Previous investigations allowed establishing PCN as a factor of fungal virulence. However, the effect PCN exerts on the yeast’s resistance to antifungal pharmacological agents used to treat human PCM are not known. Therefore, this work characterizes the role of PCN functional duality on virulence and susceptibility of P. brasiliensis to antifungal drugs. We show that the PCN overexpression increases the virulence of P. brasiliensis yeasts in an alternative model of infection, induces high susceptibility in vitro and in vivo of P. brasiliensis yeasts to antifungal therapy, and impact reducing relative mRNA expression of genes encoding proteins related to cell wall degradation. Conversely, PCN silencing minimized the yeasts’ virulence in Galleria mellonella, correlates with the lowest susceptibility to treatment with antifungal agent in vivo and impact differently from the PCN overexpression on the relative expression of markers related to P. brasiliensis yeasts cell wall remodelling. Our study demonstrates the impact of endogenous PCN on the P. brasiliensis yeasts’ virulence vs. susceptibility to antifungal drugs, the fungal biology, and the relationship of the yeasts-host cells.

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“…These same peptides also increased in the presence of the 14-3-3 protein, indicating their potential use in Paracoccidioides spp. as a future vaccine [ 151 ].…”

Section: The Caenorhabditis Elegansmentioning

confidence: 99%

Alternative Non-Mammalian Animal and Cellular Methods for the Study of Host–Fungal Interactions

Fusco-Almeida,

de Matos Silva,

dos Santos

et al. 2023

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In the study of fungal pathogenesis, alternative methods have gained prominence due to recent global legislation restricting the use of mammalian animals in research. The principle of the 3 Rs (replacement, reduction, and refinement) is integrated into regulations and guidelines governing animal experimentation in nearly all countries. This principle advocates substituting vertebrate animals with other invertebrate organisms, embryos, microorganisms, or cell cultures. This review addresses host–fungus interactions by employing three-dimensional (3D) cultures, which offer more faithful replication of the in vivo environment, and by utilizing alternative animal models to replace traditional mammals. Among these alternative models, species like Caenorhabditis elegans and Danio rerio share approximately 75% of their genes with humans. Furthermore, models such as Galleria mellonella and Tenebrio molitor demonstrate similarities in their innate immune systems as well as anatomical and physiological barriers, resembling those found in mammalian organisms.

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In Vitro and In Vivo Effect of Peptides Derived from 14-3-3 Paracoccidioides spp. Protein

Cited by 6 publications

References 75 publications

Prospecting Biomarkers for Diagnostic and Therapeutic Approaches in Pythiosis

Prospecting Biomarkers for Diagnostic and Therapeutic Approaches in Pythiosis

Paracoccin impacts on Paracoccidioides brasiliensis virulence and susceptibility to antifungal drugs by modifying the expression of genes related to remodeling of the yeasts cell wall

Alternative Non-Mammalian Animal and Cellular Methods for the Study of Host–Fungal Interactions

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