In vitro and in vivo osteogenesis of human mesenchymal stem cells derived from skin, bone marrow and dental follicle tissues

Park, Bong-Wook; Kang, Eunju; Byun, June‐Ho; Son, Myeong-Gyun; Kim, Hyun Joon; Hah, Young‐Sool; Kim, Sang Woo; Kumar, B. Mohana; Ock, Sun‐A; Rho, Gyu‐Jin

doi:10.1016/j.diff.2012.02.008

Cited by 78 publications

(70 citation statements)

References 39 publications

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“…[34][35][36] Biological components including (but not limiting to) mesenchymal stem cells (MSCs) derived from various tissues such as bone marrow, 18 adipose tissue, 37 umbilical cord, 32 muscle, 38 skin, 39 tooth, 40 and osteoblasts from different regions [41][42][43] and also several biomolecules and growth factors like bone morphogenetic protein (BMP), 37 basic fibroblast growth factor (bFGF), 41 and vascular endothelial growth factor (VEGF) 44 can be used in combination with fibrin. Different properties of these adjacent materials led to different results both in vitro and in vivo.…”

mentioning

confidence: 99%

A review of fibrin and fibrin composites for bone tissue engineering

Noori

Ashrafi

Vaez-Ghaemi

et al. 2017

IJN

377

256

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Tissue engineering has emerged as a new treatment approach for bone repair and regeneration seeking to address limitations associated with current therapies, such as autologous bone grafting. While many bone tissue engineering approaches have traditionally focused on synthetic materials (such as polymers or hydrogels), there has been a lot of excitement surrounding the use of natural materials due to their biologically inspired properties. Fibrin is a natural scaffold formed following tissue injury that initiates hemostasis and provides the initial matrix useful for cell adhesion, migration, proliferation, and differentiation. Fibrin has captured the interest of bone tissue engineers due to its excellent biocompatibility, controllable biodegradability, and ability to deliver cells and biomolecules. Fibrin is particularly appealing because its precursors, fibrinogen, and thrombin, which can be derived from the patient’s own blood, enable the fabrication of completely autologous scaffolds. In this article, we highlight the unique properties of fibrin as a scaffolding material to treat bone defects. Moreover, we emphasize its role in bone tissue engineering nanocomposites where approaches further emulate the natural nanostructured features of bone when using fibrin and other nanomaterials. We also review the preparation methods of fibrin glue and then discuss a wide range of fibrin applications in bone tissue engineering. These include the delivery of cells and/or biomolecules to a defect site, distributing cells, and/or growth factors throughout other pre-formed scaffolds and enhancing the physical as well as biological properties of other biomaterials. Thoughts on the future direction of fibrin research for bone tissue engineering are also presented. In the future, the development of fibrin precursors as recombinant proteins will solve problems associated with using multiple or single-donor fibrin glue, and the combination of nanomaterials that allow for the incorporation of biomolecules with fibrin will significantly improve the efficacy of fibrin for numerous bone tissue engineering applications.

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mentioning

confidence: 99%

A review of fibrin and fibrin composites for bone tissue engineering

Noori

Ashrafi

Vaez-Ghaemi

et al. 2017

IJN

377

256

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“…The cells were mixed with demineralized bone matrix and fibrin gel scaffolds and transplanted into the subcutaneous tissues. Four weeks after implantation, all groups had produced new bone-like tissues with high OCN expression and radio-opacity [18]. Interestingly, the DFC-grafted group exhibited higher OCN expression and calcium content compared with BMMSC and skin-derived mesenchymal stem cells, suggesting the high potential of DFC to regenerate the mineralized tissues.…”

Section: Dental Follicle Cellsmentioning

confidence: 87%

“…Furthermore, SCAP have a higher population doubling capacity and produce significantly greater amounts of mineralized matrices compared to periodontal ligament stem cells (PDLSC) [22]. A bioengineered tooth root has been developed which was composed a rootshaped HA/tricalcium phosphate (TCP) scaffold loaded with SCAP and PDLSC [18]. Three months after transplantation into an extraction socket, a layer of dentin-like tissue formed on the bioengineered tooth root surface and a PDL-like tissue that had a natural relationship with the surrounding bone was generated [20].…”

Section: Apical Papilla Stem Cellsmentioning

confidence: 99%

“…This suggests that DFC have the potential to regenerate the structures comprising the periodonium. In addition, the osteogenic potential of bone marrow-derived mesenchymal stem cells (BMMSC), skinderived mesenchymal stem cells, and DFC has been investigated using an in vivo mouse model [18]. The cells were mixed with demineralized bone matrix and fibrin gel scaffolds and transplanted into the subcutaneous tissues.…”

Section: Dental Follicle Cellsmentioning

confidence: 99%

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Is There a Role for Neural Crest Stem Cells in Periodontal Regeneration?

Tomokiyo

Hynes

Gronthos

et al. 2015

Curr Oral Health Rep

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“…Several methods have been used to demonstrate the osteogenic potential of MSC in vivo including transplantation into a calvarial bone defect [23][24][25][26][27] or subcutaneous spaces 17,[28][29][30] . The method we used was useful for examining osteogenic potential because the cells were transplanted into an environment containing bone tissue in vivo without a complicated procedure such as generating a bone defect or fracture.…”

Section: Previous Reports Have Shown That Dfc Isolated From Humanmentioning

confidence: 99%

Osteogenic Potential of Human Dental Follicle Cells on Rat Calvaria

Iwai

Kuyama

Kuboyama

et al. 2013

J. Hard Tissue Biology.

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The objective of this study was to investigate the osteogenic potential of human dental follicle cells (hDFC) in vivo. hDFC were isolated from dental follicle tissue by enzymatic digestion and cultured in growth medium (GM). hDFC were grown in a three-dimensional (3D) culture using gelatin sponges in osteogenic induction medium (OIM) or GM. The cells were transplanted onto calvaria bone of immunodeficient rats (n = 3). Hematoxylin and eosin (H-E) staining and immunohistochemistry were performed, and bone formation was analyzed with micro-computed tomography (micro-CT). H-E staining showed newly formed bone after transplantation of hDFC grown in a 3D culture in OIM. Immunohistochemistry showed BMP-2, Runx2, and Osterix expression in the hDFC transplantation site during the early stage of bone formation. Moreover, micro-CT showed that the transplanted hDFC that were 3D-cultured in OIM promoted good bone quality and bone volume compared to the other two groups. Thus, human dental follicles may be a potentially useful cell source for regenerative therapy.

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In vitro and in vivo osteogenesis of human mesenchymal stem cells derived from skin, bone marrow and dental follicle tissues

Cited by 78 publications

References 39 publications

A review of fibrin and fibrin composites for bone tissue engineering

A review of fibrin and fibrin composites for bone tissue engineering

Is There a Role for Neural Crest Stem Cells in Periodontal Regeneration?

Osteogenic Potential of Human Dental Follicle Cells on Rat Calvaria

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