In vitro and in vivo genotoxic activity of miral, an organophosphorus insecticide used in Colombia

Sierra‐Torres, Carlos H.; Cajas-Salazar, Nohelia; Hoyos, Luz Stella; Zuleta, Margarita; Whorton, Elbert B.; Au, William W.

doi:10.1016/s1383-5718(98)00054-0

Cited by 11 publications

(5 citation statements)

References 39 publications

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“…In order to establish a doseresponse relationship, 5 different concentrations of dicapthon were tested, and no induced revertants were observed along the dose range tested in either with or without S9 mix with four strains. Some of the OPs also showed a mutagenic effect in Ames test (Carver et al 1985;Kappas et al 1990;Sierra-Tores et al 1998;Karabay and Oguz 2005;Wu et al 2010). Since CAs are characterized by changes in either chromosomal structure or in the total number of chromosomes (Russel 2002), SCEs represent recombinogenic events arising at DNA lesions (Conrad et al 2011), and MN are expressed in cells when either acentric chromosome fragments of whole chromosomes fail to be segregated to the daughter nuclei during mitosis (Fenech and Bonassi 2011), they are one of the best-established cytogenetic methods for the determination of the genotoxic effects of pesticides in human peripheral blood lymphocytes (Yılmaz et al 2008;Ennaceur et al 2008;Kocaman and Topaktaş 2010;Ü nal et al 2011).…”

Section: Discussionmentioning

confidence: 99%

“…chlorpyrifos (Gollapudi et al 1995), baygon, mobile, mortein and total (Akintonwa et al 2008), and some of the them had a mutagenic effect in the Ames test, i.e. acephate (Carver et al 1985), chloracetophone (Kappas et al 1990), miral (Sierra-Tores et al 1998), methamidophos (Karabay and Oguz 2005), dicrotophos (only 5,000 lg/plate) (Wu et al 2010). Some of the OPs were also found genotoxic in human lymphocytes with different test systems i.e.…”

Section: Introductionmentioning

confidence: 99%

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Mutagenicity and genotoxicity of dicapthon insecticide

Liman

2014

Cytotechnology

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Mutagenic and genotoxic effects of dicapthon were investigated by using the bacterial reverse mutation assay in Salmonella typhimurium TA97, TA98, TA100 and TA102 strains with or without metabolic activation system (S9 mix), and chromosome aberrations (CAs), sister chromatid exchanges (SCEs), and micronucleus (MN) tests in human peripheral blood lymphocytes in vitro. Dicapthon was dissolved in dimethyl sulfoxide for all test systems. 0.1, 1, 10 and 100 lg/plate doses of dicapthon were found to be weakly mutagenic on S. typhimurium TA 98 without S9 mix. The human peripheral lymphocytes were treated with four experimental concentrations of dicapthon (25, 50, 100, and 200 lg/mL) for 24 and 48 h. Dicapthon increased the frequency of SCE only at the 100 lg/mL concentration for the 24 and 48 h applications. Dicapthon also induced abnormal cell frequency, CA/cell ratio and frequency of MN dose dependently for 24 and 48 h. Dicapthon showed a statistically significant cytotoxic effect by decreasing the mitotic index in all concentrations and a cytostatic effect by decreasing nuclear division index in 100 and 200 lg/mL concentrations for both treatment periods when compared with both untreated and solvent controls. These values decreased also in a dose dependent manner.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Mutagenicity and genotoxicity of dicapthon insecticide

Liman

2014

Cytotechnology

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“…Los afluentes que surten de agua a las plantas de potabilización pueden recibir mutágenos por medio de contaminación con pesticidas que en su mayoría han mostrado ser mutagénicos (Blair, 1995;Brown et al, 1990;Rehana et al, 1995;Sierra et al, 1998;Zuleta et al, 1990). Según Morelli (2000), más de la mitad de pesticidas presentan potencialidad carcinogénica.…”

Section: Introductionunclassified

Contribución de la contaminación y cloración en la mutagenicidad, genotoxocidad y presencia de mutágenos en agua potable

et al. 2017

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Este trabajo evaluó el efecto mutagénico y genotóxico producido por tres tipos de aguas: 1) agua sin tratar que surte una planta de potabilización, 2) agua tratada y clorada y 3) agua que llega a los domicilios; también se identificaron mutágenos presentes en el agua domiciliaria. El test de Ames encontró que el agua contaminada a pH 7 que entra a la planta presenta alta mutagenicidad indirecta superó siete veces la mutagenicidad del control, pero después de tratada, sólo produjo dos y medio veces la mutagenicidad indirecta del control. Lo anterior indica que la planta removió el 65% de la mutagenicidad procedente de la contaminación. Pero, la cloración del agua a pH 2 produjo aumento ostensible de la mutagenicidad directa en la cepa TA100 la cual superó ocho veces el control. Este mismo patrón de mutagenicidad se observó en los domicilios pero en menor escala. Los tres tipos de agua presentaron alta genotoxicidad significativa en linfocitos humanos, lo que indica que los mutágenos presentes en el agua de consumo pueden dañar el ADN de estas células, y por lo tanto, podrían implicar un factor de riesgo para la salud humana. Un análisis por cromatografía de gas acoplado a espectrometría de masas (GC/MS) detectó la existencia de cinco hidrocarburos policíclicos aromáticos en el agua domiciliaria: Naftaleno 1,1’(1,10 decanodil) bis, fenantreno 9 dodeciltetradecahidro, dibenzo(a,h)antraceno 1,2,3,4 tetrahidro, benz(a)antraceno 7,12 dimetil y 9, 9’ bifenantreno octacosahidro de los cuales, los primeros cuatro son mutacarcinógenos reconocidos. También se identificó la hormona esteroidea androstane 5 alfa que podría ser promotora del proceso de carcinogénesis.

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“…Although the marked alteration of nucleic acid metabolism, including the genotoxic and carcinogenic effects, was mostly described for less toxic organophosphorus pesticides (5,16,20), there are some results demonstrating the possible effects of highly toxic OPs on the metabolism of nucleic acids as well as proteins that can lead to the signs of genotoxicity. OP agent tabun was described as weakly acting mutagen using Salmonella spp.…”

Section: Introductionmentioning

confidence: 99%

The Long Term Changes in Liver DNA and Total Protein Contents Following Low Level Sarin Exposure in Rats

Kassa

Skopec

Vachek

2000

Acta Med. (Hradec Kralove, Czech Repub.)

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1. The changes in contents of DNA and total protein in the liver of the rats exposed to low level sarin by inhalation at 3, 6 and 12 months following the exposure were studied. The influence of sarin on the DNA and protein metabolism in liver was determinated by the measurements of incorporation of tritiated thymidine into DNA, the concentration of DNA and total protein. 2. Our results show that not only symptomatic level 3 but also asymptomatic levels 1 and 2 of sarin are able to significantly decrease the incorporation of radiolabelled thymidine without changing total concentrations of DNA as well as protein at three months following sarin exposure. On the other hand, the significant decrease in total contents of DNA and protein in liver without the changes in the incorporation of tritiated thymidine was determined in liver six months following sarin exposure. Practically no significant changes in the metabolism of DNA and protein were observed at 12 months following sarin exposure. 3. Thus, not only clinically manifested intoxication but also low-level, asymptomatic exposure to nerve agents such as sarin is able to influence the metabolism of nucleic acids as well as proteins even several months following the exposure.

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In vitro and in vivo genotoxic activity of miral, an organophosphorus insecticide used in Colombia

Cited by 11 publications

References 39 publications

Mutagenicity and genotoxicity of dicapthon insecticide

Mutagenicity and genotoxicity of dicapthon insecticide

Contribución de la contaminación y cloración en la mutagenicidad, genotoxocidad y presencia de mutágenos en agua potable

The Long Term Changes in Liver DNA and Total Protein Contents Following Low Level Sarin Exposure in Rats

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