In vitro and in vivo ameliorative effects of polyphenols from purple potato leaves on renal injury and associated inflammation induced by hyperuricemia

Abstract

In the present study, the ameliorative effects of polyphenols from purple potato leaves (PSPLP) on hyperuricemia were investigated. HPLC-MS analysis showed that PSPLP was mainly composed of caffeoylquinic acid derivatives (84%). PSPLP inhibited the levels of cytokines (IL-1β, IL-6, and TNFα) in monosodium urate-induced RAW264.7 cells. In vivo, PSPLP significantly inhibited the level of uric acid in hyperuricemia mice from 209.6 to 166.6 μM, and significantly interfered with the activities of xanthine oxidase (XOD) and adenosine deaminase in liver, the activity of XOD decreased from 13.5 to 11.6 U/gprot. PSPLP can decrease serum creatinine level from 105 to 59 μM, and urea nitrogen level from 21.9 to 14.1 mM, which can effectively protect kidney.These results provide a reference for future research and application of PSPLP as a functional food to intervene hyperuricemia and associated inflammation. Practical applicationsThis study evaluated the effect of polyphenols from purple potato leaves (PSPLP) on hyperuricemia. The results suggested that PSPLP has an important role in the intervention of hyperuricemia and hyperuricemic-related inflammation or renal injury, and can be used in the application of functional foods. These results provided a basis for further study on the biological activities of polyphenols from purple sweet potato leaves.

In Vitro Gastrointestinal Digestion of Various Sweet Potato Leaves: Polyphenol Profiles, Bioaccessibility and Bioavailability Elucidation

“…The following supporting information can be downloaded at: https: //www.mdpi.com/article/10.3390/antiox13050520/s1, Figure S1: The chemical composition of SPL; Table S1: Compounds identification, compound formula, retention times, measured m/z of molecular and mass fragments (MS 2 ) in SPL; Table S2: Content changes in the phenolic profile in SPLs after each digestion stage. References [60][61][62][63] are cited in the supplementary materials.…”

Anti-Inflammatory and Hypouricemic Effect of Bioactive Compounds: Molecular Evidence and Potential Application in the Management of Gout

“…Reduced production of other pro-inflammatory mediators, such as COX-2 and IL-8, induced by MSU crystal has been observed in different cell types cultured with pycnogenol and morin by inactivation of NF-kB signaling pathway ( Table 1 ) [ 81 , 82 ]. Decreased levels of pro-inflammatory cytokines (IL-1β, IL-6, and TNF-α) have been determined also in MSU crystal-stimulated macrophages after treatment with polyphenols from purple potato leaves [ 83 ].…”

Quercetin ameliorates hyperuricemic nephropathy by repressing uric acid synthesis and reabsorption in mice and cells

“…Previous study indicated that some naturally substance can decrease the UA level in vivo and in vitro (Li et al, 2021; Xiang et al, 2021; Xu et al, 2021). Such as, Camellia japonica bee pollen and potato leaves polyphenols markedly reduce serum UA content via inhibiting UA reabsorption and production in hyperuricemia mice (Sun et al, 2022; Xu et al, 2021). Suitable apigenin (API) treatment could markedly decrease the level of serum UA content by inhibiting the reabsorption in hyperuricemia mice (Li et al, 2021).…”