In vitro and in vivo evaluation of an electrospun-aligned microfibrous implant for Annulus fibrosus repair

Gluais, Maude; Clouet, Johann; Fusellier, Marion; Decante, Cyrille; Moraru, C.; Dutilleul, Maéva; Véziers, Joëlle; Lesoeur, Julie; Dumas, Dominique; Abadie, Jérôme; Hamel, Antoine; Bord, E.; Chew, Sing Yian; Guicheux, Jérôme; Visage, Catherine Le

doi:10.1016/j.biomaterials.2019.03.010

Cited by 78 publications

(69 citation statements)

References 70 publications

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“…PCL is FDA-approved, biodegradable, and protocols for electrospinning PCL are well-established. Furthermore, numerous in vivo studies support the biocompatibility of PCL [19][20][21]. Other synthetic polymers used in electrospinning include poly (3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) [22], poly (vinylidene fluoride) (PVD) [23], and poly (lactic acid) (PLA) [24].…”

Section: Introductionmentioning

confidence: 99%

3D printed mesh reinforcements enhance the mechanical properties of electrospun scaffolds

et al. 2019

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Background: There is substantial interest in electrospun scaffolds as substrates for tissue regeneration and repair due to their fibrous, extracellular matrix-like composition with interconnected porosity, cost-effective production, and scalability. However, a common limitation of these scaffolds is their inherently low mechanical strength and stiffness, restricting their use in some clinical applications. In this study we developed a novel technique for 3D printing a mesh reinforcement on electrospun scaffolds to improve their mechanical properties. Methods: A poly (lactic acid) (PLA) mesh was 3D-printed directly onto electrospun scaffolds composed of a 40:60 ratio of poly(ε-caprolactone) (PCL) to gelatin, respectively. PLA grids were printed onto the electrospun scaffolds with either a 6 mm or 8 mm distance between the struts. Scanning electron microscopy was utilized to determine if the 3D printing process affected the archtitecture of the electrospun scaffold. Tensile testing was used to ascertain mechanical properties (strength, modulus, failure stress, ductility) of both unmodified and reinforced electrospun scaffolds. An in vivo bone graft model was used to assess biocompatibility. Specifically, reinforced scaffolds were used as a membrane cover for bone graft particles implanted into rat calvarial defects, and implant sites were examined histologically. Results: We determined that the tensile strength and elastic modulus were markedly increased, and ductility reduced, by the addition of the PLA meshes to the electrospun scaffolds. Furthermore, the scaffolds maintained their matrix-like structure after being reinforced with the 3D printed PLA. There was no indication at the graft/tissue interface that the reinforced electrospun scaffolds elicited an immune or foreign body response upon implantation into rat cranial defects. Conclusion: 3D-printed mesh reinforcements offer a new tool for enhancing the mechanical strength of electrospun scaffolds while preserving the advantageous extracellular matrix-like architecture. The modification of electrospun scaffolds with 3D-printed reinforcements is expected to expand the range of clinical applications for which electrospun materials may be suitable.

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Section: Introductionmentioning

confidence: 99%

3D printed mesh reinforcements enhance the mechanical properties of electrospun scaffolds

et al. 2019

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“…A recent study assessed the potential of a biomimetic multilayer PCL fibrous scaffold to repair AF defects in an ovine lumbar model. Results showed that electrospun PCL successfully integrated within the AF tissue, promoting cell infiltration and deposition of oriented collagen fibers within the aligned scaffold 94 . Another study focused on the implantation of an endplate‐modified DAPS (eDAPS) into a goat cervical disc replacement model 95 .…”

Section: Electrospinningmentioning

confidence: 99%

Electrospinning and 3D bioprinting for intervertebral disc tissue engineering

et al. 2020

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Intervertebral disc (IVD) degeneration is a major cause of low back pain and represents a massive socioeconomic burden. Current conservative and surgical treatments fail to restore native tissue architecture and functionality. Tissue engineering strategies, especially those based on 3D bioprinting and electrospinning, have emerged as possible alternatives by producing cell-seeded scaffolds that replicate the structure of the IVD extracellular matrix. In this review, we provide an overview of recent advancements and limitations of 3D bioprinting and electrospinning for the treatment of IVD degeneration, focusing on future areas of research that may contribute to their clinical translation.

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“…[ 3 ] Moreover, the compact fibrous structures within electrospun scaffolds were found to inhibit cell infiltration into the scaffold and result in deficient tissue regeneration. [ 4 ] These considerations have led to the fabrication of 3D scaffolds with well‐defined microscale structural patterns, such as oriented micropores and accordion‐like honeycombs, in order to control cell alignment and organization in the 3D environment. [ 5 ]…”

Section: Figurementioning

confidence: 99%

“…For each fork, the tilted branch delivered oxygen and nutrients in a new direction and covered more space, while the other parallel branch aligned with the oriented micropores to maintain the structural anisotropy of the scaffold. Generally, the relationship between the diameters of mother and daughter branches in physiology can be described as shown in Equation () [ 4 ]

\begin{matrix} true \\ right D_{0}^{γ} = D_{1}^{γ} + D_{2}^{γ} \end{matrix}

\begin{matrix} true \\ right τ_{w} = η \frac{4 U}{R_{normalc}} \end{matrix}

where D 0 is the diameter of the mother branch, D 1 and D 2 are the diameters of the daughter branches, γ is exponential index, τ w is the wall shear stress, η is the culture medium viscosity, U is the average fluid velocity of the culture medium in the channel, and R c is the channel radius.…”

Section: Figurementioning

confidence: 99%

Optimizing Bifurcated Channels within an Anisotropic Scaffold for Engineering Vascularized Oriented Tissues

Fang

Ouyang

Zhang

et al. 2020

Adv Healthcare Materials

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Despite progress in engineering both vascularized tissues and oriented tissues, the fabrication of 3D vascularized oriented tissues remains a challenge due to an inability to successfully integrate vascular and anisotropic structures that can support mass transfer and guide cell alignment, respectively. More importantly, there is a lack of an effective approach to guiding the scaffold design bearing both structural features. Here, an approach is presented to optimize the bifurcated channels within an anisotropic scaffold based on oxygen transport simulation and biological experiments. The oxygen transport simulation is performed using the experimentally measured effective oxygen diffusion coefficient and hydraulic permeability of the anisotropic scaffolds, which are also seeded with muscle precursor cells and cultured in a custom‐made perfusion bioreactor. Symmetric bifurcation model is used as fractal unit to design the channel network based on biomimetic principles. The bifurcation level of channel network is further optimized based on the oxygen transport simulation, which is then validated by DNA quantification assay and pimonidazole immunostaining. This study provides a practical guide to optimizing bifurcated channels in anisotropic scaffolds for oriented tissue engineering.

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In vitro and in vivo evaluation of an electrospun-aligned microfibrous implant for Annulus fibrosus repair

Cited by 78 publications

References 70 publications

3D printed mesh reinforcements enhance the mechanical properties of electrospun scaffolds

3D printed mesh reinforcements enhance the mechanical properties of electrospun scaffolds

Electrospinning and 3D bioprinting for intervertebral disc tissue engineering

Optimizing Bifurcated Channels within an Anisotropic Scaffold for Engineering Vascularized Oriented Tissues

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