In vitro and in vivo evaluation of differentially demineralized cancellous bone scaffolds combined with human bone marrow stromal cells for tissue engineering

Mauney, Joshua R.; Jaquiéry, Claude; Volloch, Vladimir; Heberer, Michael; Martin, Ivan; Kaplan, David L.

doi:10.1016/j.biomaterials.2004.08.020

Cited by 166 publications

(116 citation statements)

References 74 publications

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“…Non-seeded scaffolds and tissue culture wells were also maintained in culture medium as above and were analyzed similarly as blank controls to adjust for background fluorescence. Scaffolds were then weighed and the relative cell numbers were calculated as the degree of their relative fluorescence intensity (RFU) per mg of scaffold wet weight as previously described [33].…”

Section: Relative Cell Numbersmentioning

confidence: 99%

“…Seeded scaffolds cultivated in the presence of AD as detailed above were evaluated for their ability to support in vivo adipogenesis in a small animal muscle pouch model as previously described [33]. Scaffolds were individually implanted into bilateral muscle pouches within the rectus abdominus muscles of athymic nude male rats (RH-rnu, ~300 g each) and maintained there for 4 weeks.…”

Section: In Vivo Implantationmentioning

confidence: 99%

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Engineering adipose-like tissue in vitro and in vivo utilizing human bone marrow and adipose-derived mesenchymal stem cells with silk fibroin 3D scaffolds

et al. 2007

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Biomaterials derived from silk fibrion prepared by aqueous (AB) and organic (HFIP) solvent based processes, along with collagen (COL) and poly-lactic acid (PLA) based scaffolds were studied in vitro and in vivo for their utility in adipose tissue engineering strategies. For in vitro studies, human bone marrow and adipose-derived mesenchymal stem cells (hMSCs and hASCs) were seeded on the various biomaterials and cultured for 21 days in the presence of adipogenic stimulants (AD) or maintained as noninduced controls. Alamar Blue analysis revealed each biomaterial supported initial attachment of hMSCs and hASCs to similar levels for all matrices except COL in which higher levels were observed. hASCs and hMSCs cultured on all biomaterials in the presence of AD showed significant upregulation of adipogenic mRNA transcript levels (LPL, GLUT4, FABP4, PPARγ, adipsin, ACS) to similar extents when compared to noninduced controls. Similarly Oil-Red O analysis of hASC or hMSC-seeded scaffolds displayed substantial amounts of lipid accumulating adipocytes following cultivation with AD. The data revealed AB and HFIP scaffolds supported similar extents of lipid accumulating cells while PLA and COL scaffolds qualitatively displayed lower and higher extents by comparison, respectively. Following a 4 week implantation period in a rat muscle pouch defect model, both AB and HFIP scaffolds supported in vivo adipogenesis either alone or seeded with hASCs or hMSCs as assessed by Oil-Red O analysis, however the presence of exogenous cell sources substantially increased the extent and frequency of adipogenesis observed. In contrast, COL and PLA scaffolds underwent rapid scaffold degradation and were irretrievable following the implantation period. The results suggest that macroporous 3D AB and HFIP silk fibroin scaffolds offer an important platform for cell-based adipose tissue engineering applications, and in particular, provide longer-term structural integrity to promote the maintenance of soft tissue in vivo.

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Section: Relative Cell Numbersmentioning

confidence: 99%

Section: In Vivo Implantationmentioning

confidence: 99%

Engineering adipose-like tissue in vitro and in vivo utilizing human bone marrow and adipose-derived mesenchymal stem cells with silk fibroin 3D scaffolds

et al. 2007

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“…Although the latter presents a major potential for skeletal regeneration procedures, most in vivo studies are conducted using an ectopic approach and/or performed in small animal models, such as mice or rats (Kruyt et al, 2007;Livington et al, 2002;Mauney et al, 2005a;Mastrogiacomo et al, 2007;Mendes et al, 2003;Trojani et al, 2006). Although non-critical sized defects are usually evaluated in ectopic models, orthotopic location provides a more accurate idea of the influence or local effects of implanted cells or cell-scaffold constructs where they were initially designed to be functional.…”

Section: Introductionmentioning

confidence: 99%

Tissue-engineered constructs based on SPCL scaffolds cultured with goat marrow cells: functionality in femoral defects

Rodrigues

Gomes

Viegas

et al. 2010

J Tissue Eng Regen Med

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This study aims to assess the in vivo performance of cell-scaffold constructs composed of goat marrow stromal cells (GBMCs) and SPCL (a blend of starch with polycaprolactone) fibre mesh scaffolds at different stages of development, using an autologous model. GBMCs from iliac crests were seeded onto SPCL scaffolds and in vitro cultured for 1 and 7 days in osteogenic medium. After 1 and 7 days, the constructs were characterized for proliferation and initial osteoblastic expression by alkaline phosphatase (ALP) activity. Scanning electron microscopy analysis was performed to investigate cellular morphology and adhesion to SPCL scaffolds. Non-critical defects (diameter 6 mm, depth 3 mm) were drilled in the posterior femurs of four adult goats from which bone marrow and serum had been collected previously. Drill defects alone and defects filled with scaffolds without cells were used as controls. After implantation, intravital fluorescence markers, xylenol orange, calcein green and tetracycline, were injected subcutaneously after 2, 4 and 6 weeks, respectively, for bone formation and mineralization monitoring. Subsequently, samples were stained with Lévai-Laczkó for bone formation and histomorphometric analysis. GBMCs adhered and proliferated on SPCL scaffolds and an initial differentiation into pre-osteoblasts was detected by an increasing level of ALP activity with the culture time. In vivo experiments indicated that bone neoformation occurred in all femoral defects. The results obtained provided important information about the performance of SPCL-GBMC constructs in an orthotopic goat model that enabled future studies to be designed to investigate in vivo the functionality of SPCL-GBMC constructs in more complex models, viz. critical sized defects, and to evaluate the influence of in vitro cultured autologous cells in the healing and bone regenerative process.

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“…Several investigations have shown that calcium phosphate surfaces have a beneficial effect on bone osteogenesis in vivo (Lee et al, 2000;Uemura et al, 2003;Mauney et al, 2005). These reports cannot exclude the possibility that recipient-derived cells were contained in the transplantation of scaffolds for regeneration of bone.…”

Section: Discussionmentioning

confidence: 99%

Mature Adipocyte-Derived Dedifferentiated Fat Cells Can Trans-Differentiate into Osteoblasts <i>In Vitro</i> and <i>In Vivo</i> only by All-Trans Retinoic Acid

Oki

Watanabe

Endo

et al. 2008

Cell Struct. Funct.

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ABSTRACT. We investigated whether de-differentiated fat (DFAT) cells, a mature adipocyte-derived preadipocyte cell line, can be induced to trans-differentiate into osteoblasts in vitro and in vivo. All-trans retinoic acid (RA) induced expression of osteoblast-specific mRNAs encoding Cbfa1/Runx2, osterix, alkaline phosphatase, osteopontin, parathyroid hormone receptor, and osteocalcin in the DFAT cells, but did not induce the expression of adipocytespecific mRNAs encoding PPARγ2, C/EBPα, and GLUT4. Moreover, alkaline phosphatase activity was expressed in DFAT cells and the cells underwent mineralization of the bone matrix in vitro. Furthermore, when DFAT cells were transplanted subcutaneously into C57BL/6N mice in diffusion chambers, these cells formed ectopic osteoid tissue without any host cell-invasion of the chambers. These results indicate that DFAT cells derived from mature adipocytes can be converted into fully differentiated osteoblasts in vitro and in vivo using RA. DFAT cells provide a unique model for studying the lineage commitment of the adipocytes and osteoblasts derived from mesenchymal stem cells. Identification of the pathways that regulate these processes could lead to the development of new therapeutic strategies for control of unwarranted growth of bone and adipose tissue.

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In vitro and in vivo evaluation of differentially demineralized cancellous bone scaffolds combined with human bone marrow stromal cells for tissue engineering

Cited by 166 publications

References 74 publications

Engineering adipose-like tissue in vitro and in vivo utilizing human bone marrow and adipose-derived mesenchymal stem cells with silk fibroin 3D scaffolds

Engineering adipose-like tissue in vitro and in vivo utilizing human bone marrow and adipose-derived mesenchymal stem cells with silk fibroin 3D scaffolds

Tissue-engineered constructs based on SPCL scaffolds cultured with goat marrow cells: functionality in femoral defects

Mature Adipocyte-Derived Dedifferentiated Fat Cells Can Trans-Differentiate into Osteoblasts <i>In Vitro</i> and <i>In Vivo</i> only by All-Trans Retinoic Acid

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