In vitro and in vivo inhibition of HCT116 cells by essential oils from bark and leaves of Virola surinamensis (Rol. ex Rottb.) Warb. (Myristicaceae)

Anunciação, Talita Andrade da; Costa, Rafaela G.A.; Lima, Emilly J. S. P. de; Silva, Valdenizia R.; Santos, Luciano de S.; Soares, Milena Botelho Pereira; Dias, Rosane Borges; Rocha, Clarissa Araújo Gurgel; Costa, Emmanoel V.; Silva, Felipe M. A. da; Koolen, Héctor H. F.; Bezerra, Daniel P.

doi:10.1016/j.jep.2020.113166

Cited by 11 publications

(8 citation statements)

References 39 publications

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“…Animals were grafted subcutaneously with HepG2 cells (10 7 cells per 500 μL), in the left armpit and randomly divided into four groups: (G1) vehicle‐treated mice (5 % DMSO, n= 20); (G2) 5‐FU‐treated mice (10 mg/kg, n =10); (G3) EO‐treated mice (40 mg/kg, n =10); and (G4) EO‐treated mice (80 mg/kg, n =10). These doses were based on previous studies with EO [33–37] . Starting one day after tumor implantation, the animals were treated intraperitoneally for 21 consecutive days.…”

Section: Methodsmentioning

confidence: 99%

Essential Oil from Bark of Aniba parviflora (Meisn.) Mez (Lauraceae) Reduces HepG2 Cell Proliferation and Inhibits Tumor Development in a Xenograft Model

Oliveira

Rodrigues

Lima

et al. 2021

Chemistry & Biodiversity

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Aniba parviflora (Meisn.) Mez (Lauraceae) is an aromatic plant of the Amazon rainforest, which has a tremendous commercial value in the perfumery industry; it is popularly used as flavoring sachets and aromatic baths. In Brazilian folk medicine, A. parviflora is used to treat victims of snakebites. Herein, we analyzed the chemical composition of A. parviflora bark essential oil (EO) and its effect on the growth of human hepatocellular carcinoma HepG2 cells in vitro and in vivo. EO was obtained by hydrodistillation and characterized by GC‐MS and GC‐FID. The main constituents of EO were linalool (16.3±3.15), α‐humulene (14.5±2.41 %), δ‐cadinene (10.2±1.09 %), α‐copaene (9.51±1.12 %) and germacrene B (7.58±2.15 %). Initially, EO's cytotoxic effect was evaluated against five cancer cell lines (HepG2, MCF‐7, HCT116, HL‐60 and B16‐F10) and one non‐cancerous one (MRC‐5), using the Alamar blue method after 72 h of treatment. The calculated IC50 values were 9.05, 22.04, >50, 15.36, 17.57, and 30.46 μg/mL, respectively. The best selectivity was for HepG2 cells with a selective index of 3.4. DNA Fragmentation and cell cycle distribution were quantified in HepG2 cells by flow cytometry after a treatment period of 24 and 48 h. The effect of EO on tumor development in vivo was evaluated in a xenograft model using C.B‐17 SCID mice engrafted with HepG2 cells. In vivo tumor growth inhibition of HepG2 xenograft at the doses of 40 and 80 mg/kg were 12.1 and 62.4 %, respectively.

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Section: Methodsmentioning

confidence: 99%

Essential Oil from Bark of Aniba parviflora (Meisn.) Mez (Lauraceae) Reduces HepG2 Cell Proliferation and Inhibits Tumor Development in a Xenograft Model

Oliveira

Rodrigues

Lima

et al. 2021

Chemistry & Biodiversity

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“…Our analyses demonstrated that the collection time did not influence the yield of essential oil; however, higher yields were achieved in the dry season (September), which correspond to the period of lower incidence of rainfall (Table 1). In previous studies, Anunciação et al (2020) [2] reported yields equivalent to 1.21 ± 0.10% (bark) and 1.78 ± 0.17% (leaves) of the V. surinamensis essential oil collected in the Amazon region (Amazonas state, Brazil). Several factors can influence the yield of essential oils, such as seasonal and circadian variations [34,35].…”

Section: Yield and Chemical Composition Of The Essential Oilsmentioning

confidence: 97%

“…In another study, Anunciação et al (2020) [2] evaluated the inhibition of essential oil samples obtained from bark and leaves of V. surinamensis against HCT116 carcinoma cells using in vitro and in vivo assays. ey reported as major compounds the β-elemene (9.61 ± 1.02%), bicyclogermacrene (8.1 ± 2.0%), germacrene D (7.44 ± 1.80%), α-cubebene (5.69 ± 0.67%), and α-copaene (5.02 ± 0.77%); they indicated that some of these major compounds of V. surinamensis essential oil can be an alternative in the development of anticancer compounds to treat the colon cancer.…”

Section: Yield and Chemical Composition Of The Essential Oilsmentioning

confidence: 99%

“…V. surinamensis is an Amazon species, which habits the floodplain and "igapós." In the Amazon region, it is popularly known by its Portuguese names Ucuúba, Sucuúba, Súcuba, and Leite-de-mucuiba [2,3]. It is described by its medicinal properties in the treatment of infections and inflammations [4,5].…”

Section: Introductionmentioning

confidence: 99%

“…had activity against the infection caused by the methicillin-resistant strain of S. aureus. e essential oil of V. surinamensis was reported by its antimalarial activity, which was associated with the sesquiterpene nerolidol [5,8]; antitumoral activity in colon cancer cells, which was investigated using the essential oils from bark and leaves, both composed mostly by the sesquiterpenes aristolene, β-elemene, and byciclogermacrene [2]. To the best of our knowledge, there are no studies about the use of the essential oil of V. surinamensis as an antibacterial agent.…”

Section: Introductionmentioning

confidence: 99%

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In Vitro Antibacterial Activity and in Silico Analysis of the Bioactivity of Major Compounds Obtained from the Essential Oil of Virola surinamensis Warb (Myristicaceae)

Almeida

Nascimento

Costa

et al. 2022

Journal of Food Quality

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Essential oils are well known for their antimicrobial activity and they are used as an effective food preservative. Virola is one of the five genera of Myristicaceae and this genus is native to the American continent, especially in neotropical regions. The largest number of species of this genus is found in the Amazon region and the most important species include Virola surinamensis Warb. and Virola sebifera Aubl. In the present study, we describe the chemical composition of the essential oil of the V. surinamensis obtained at two different periods of the day in two seasons (rainy and dry), as well as their antimicrobial activity against pathogenic bacterial strains of Pseudomonas aeruginosa, Escherichia coli, and Staphylococcus aureus. In addition, we investigated, using in silico tools, the antimicrobial activity of the major chemical compounds present in the essential oil of V. surinamensis. The samples collected at different seasons and times showed a similar chemical profile, characterized by the major constituents α-pinene (>33%) and β-pinene (>13%). The essential oil of V. surinamensis showed an interesting antibacterial activity, exhibiting low inhibitory concentrations against the tested bacterial species. The computational investigation indicated that limonene, myrcene, and β-pinene could be related to the antibacterial activity against the tested pathogenic bacterial strains. Our results shed light on the possible constituents of essential oil that could be related to its activity against bacterial species and might be useful for further experimental tests that aim to discover new potential antibacterial agents for food preservation.

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Essential oils as anticancer agents

Jagatap¹,

Ahmad²,

Kaikini³

et al. 2023

Recent Frontiers of Phytochemicals

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In vitro and in vivo inhibition of HCT116 cells by essential oils from bark and leaves of Virola surinamensis (Rol. ex Rottb.) Warb. (Myristicaceae)

Cited by 11 publications

References 39 publications

Essential Oil from Bark of Aniba parviflora (Meisn.) Mez (Lauraceae) Reduces HepG2 Cell Proliferation and Inhibits Tumor Development in a Xenograft Model

Essential Oil from Bark of Aniba parviflora (Meisn.) Mez (Lauraceae) Reduces HepG2 Cell Proliferation and Inhibits Tumor Development in a Xenograft Model

In Vitro Antibacterial Activity and in Silico Analysis of the Bioactivity of Major Compounds Obtained from the Essential Oil of Virola surinamensis Warb (Myristicaceae)

Essential oils as anticancer agents

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