In Vitro and In Vivo Antibacterial and Antifungal Screening of Natural Plant Products: Prospective Standardization of Basic Methods

Lima‐Filho, José Vitor; Cordeiro, Rossana de Aguiar

doi:10.1007/978-1-4614-8636-7_17

Cited by 9 publications

(7 citation statements)

References 39 publications

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“…The Brazilian floras are worldwide known as a source of biologically active compounds with biodegradable properties, and, therefore, screening of plant extracts is often a first step procedure for isolation of phytochemicals [ 23 ]. However, studies that correlate antibacterial activity of extracts to the presence of specific compounds are rare.…”

Section: Resultsmentioning

confidence: 99%

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Phytochemistry and Preliminary Assessment of the Antibacterial Activity of Chloroform Extract of Amburana cearensis (Allemão) A.C. Sm. against Klebsiella pneumoniae Carbapenemase‐Producing Strains

Sá

Ralph

Nascimento

et al. 2014

Evidence-Based Complementary and Alternative Medicine

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The chloroform extract of the stem bark of Amburana cearensis was chemically characterized and tested for antibacterial activity.The extract was analyzed by gas chromatography and mass spectrometry. The main compounds identified were 4-methoxy-3-methylphenol (76.7%), triciclene (3.9%), α-pinene (1.0%), β-pinene (2.2%), and 4-hydroxybenzoic acid (3.1%). Preliminary antibacterial tests were carried out against species of distinct morphophysiological characteristics: Escherichia coli, Salmonella enterica Serotype Typhimurium, Pseudomonas aeruginosa, Staphylococcus aureus, Listeria monocytogenes, and Bacillus cereus. The minimum inhibitory concentration (MIC) was determinate in 96-well microplates for the chloroform extract and an analogue of themain compound identified, which was purchased commercially.We have shown that plant's extract was only inhibitory (but not bactericidal) at the maximum concentration of 6900 μg/mL against Pseudomonas aeruginosa and Bacillus cereus. Conversely, the analogue 2-methoxy-4-methylphenol produced MICs ranging from215 to 431 μg/mL against all bacterial species.New antibacterial assays conducted with such chemical compound against Klebsiella pneumoniae carbapenemase-producing strains have shown similarMICresults and minimumbactericidal concentration (MBC) of 431 μg/mL.We conclude that A. cearensis is a good source of methoxy-methylphenol compounds,which could be screened for antibacterial activity againstmultiresistant bacteria fromdifferent species

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Section: Resultsmentioning

confidence: 99%

“…The extract was filtered and concentrated in a rotary evaporator under reduced pressure at a temperature of 45°C for complete solvent removal. Stock solutions were prepared with extracts using dimethyl sulfoxide (DMSO) as solvent (100 mg/mL), which were kept in a refrigerator at −20°C until use [ 23 ].…”

Section: Methodsmentioning

confidence: 99%

Phytochemistry and Preliminary Assessment of the Antibacterial Activity of Chloroform Extract of Amburana cearensis (Allemão) A.C. Sm. against Klebsiella pneumoniae Carbapenemase‐Producing Strains

Sá

Ralph

Nascimento

et al. 2014

Evidence-Based Complementary and Alternative Medicine

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“…For standardization, the prepared solution was adjusted to an absorbance reading range of 0.08 to 0.1 at 625nm using a spectrophotometer. 23,24 Before bacterial and fungal broth suspension preparation, all bacteria were subcultured in NA slants and incubated at 35°C for 18-24 hours. Meanwhile, all fungi were maintained and subcultured in Potato Dextrose Agar (PDA) slants and incubated at 35°C for five days.…”

Section: Antimicrobial Activity Assaymentioning

confidence: 99%

“…The swab method was employed to inoculate the 200μL fungal suspension to the Potato Dextrose Agar. 23 Three types of disks were separately prepared by impregnating 6 mm sterile Whatman no. 1 filter paper disks with sterile water as negative control, streptomycin as positive control for bacteria and fluconazole for fungi, and the crude MeOH extract.…”

Section: Antimicrobial Activity Assaymentioning

confidence: 99%

Zoochemical Analyses and In vitro Antimicrobial Activity of Crude Methanolic Extract of Perna viridis

2021

Acta Med Philipp

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Introduction. The rise of antibiotic resistance and superbugs drives the search for new antibiotics today. Meanwhile, the green mussel Perna viridis is a cultivated and marketed staple bivalve in the Philippines due to its fast reproduction, high protein content, and tolerance to environmental variables. Although some studies have analyzed the antimicrobial activity of P. viridis, zoochemical analyses and further evaluation of its antimicrobial activity, such as determining the minimum inhibitory concentration (MIC), remains unexplored.Objectives. The study evaluated the zoochemicals present in crude methanolic extract of P. viridis by qualitative screening and thin-layer chromatography analysis. It further evaluated the crude extract for its antimicrobial activity against common pathogenic bacteria and plant pathogenic fungi. Materials and Methods.The zoochemicals in crude methanolic extract of P. viridis were screened using qualitative spotting methods and thin-layer chromatography (TLC). The antimicrobial activity of the extract was evaluated against the bacteria Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, and Bacillus subtilis, and the fungi Colletotrichum capsici, Lasiodiplodia theobromae, and Rhizopus sp. using disk diffusion assay and two-fold microdilution.Results. Qualitative screening and thin-layer chromatography analysis of the crude extract revealed detectable amounts of alkaloids, saponins, terpenoids, sterols, and polyphenols. All of the tested bacteria were susceptible to the extract with P. aeruginosa (19.00±0.82 mm) and S. aureus (19.33±0.47 mm) as the most inhibited with MICs of 2.60±0.63 and 3.65±1.69 mg/mL, respectively. However, for the three fungi tested, only the growth of the fungus L. theobromae (7.33±0.94 mm) was inhibited with a MIC of 33.33±11.79 mg/mL. Conclusion.It can be inferred that the zoochemicals detected in the crude extract of P. viridis contributed to its antimicrobial activity.

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“…Antibacterial and antifungal activity of analyzed snowberries taxa -Antibakterijska i antifungalna aktivnost istraživanih svojti biseraka Plants are intensively explored, primarily based on ethnobotanical data, to confirm their antimicrobial properties and to isolate new compounds with these properties (LIMA-FILHO and CORDEIRO, 2014). Many authors agree that plant antibacterial activity is a consequence of the action of various chemical agents (active antimicrobial compounds) in their extracts, including essential oils, flavonoids, triterpenoids, other phenolic nature compounds and free hydroxyl compounds (MORRIS et al, 1979;CAKIR et al, 2004;SARAVANAKUMAR et al, 2014).…”

Section: Antioxidative Activity Of Analyzed Symphoricarpos Taxa -Antioksidativna Aktivnost Istraživanih Svojti Symphoricarposmentioning

confidence: 99%

Total Phenolics, Antioxidative and Antimicrobial Activity of Methanol Extracts of Berries of Symphoricarpos Albus (L.) S.F.BLAKE, S. × Chenaultii Rehder and S. Orbiculatus Moench

Pustahija

Subašić

Mulić³

et al. 2018

RŠF

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581.19:547.56]:582.971.1 Methanol extracts obtained from the berries of three Symphoricarpos taxa (Caprifoliaceae) were used to determine their total phenols concentrations, and antioxidant and antimicrobial activities. According to our knowledge, total concentrations of phenolic compounds and antimicrobial activities for of S. × chenaultii and S. orbiculatus, as well as antioxidant activity of all three analyzed taxa are for the first time analyzed in this study. Based on obtained results, it was noticed that the highest average values of the total phenols, flavonoids and flavanols had methanol extracts of S. orbiculatus berries (3.525 mg GAE g-1 DW, and 11.037 and 0.881 mg CE g-1 DW, respectively), while methanol extracts of S. albus berries had the lowest (1.430 mg GAE g-1 DW, and 4.154 and 0.211 mg CE g-1 DW respectively). Symphoricarpos orbiculatus extract (IC50 = 5.299 mg mL-1) possessed the highest antioxidant activity while the lowest had S. albus (IC50 = 20.017 mg mL-1). The methanol extracts of analyzed Symphoricarpos taxa showed no antifungal activity against C. albicans. Moderate antibacterial activity of S. albus methanol extract is determined against five tested bacterial strains (Bacillus vulgatus, Enterococcus faecalis, Pseudomonas aeruginosa, Salmonella abony, and Staphylococcus aureus), and S. × chenaultii extract showed a low antibacterial activity only against P. aeruginosa, B. vulgatus and S. aureus. Although S. orbiculatus methanolic extract had the highest antioxidant activity it did not show any antibacterial activity at all. Analyzed Symphoricarpos extracts are potentially effective and can be used as natural antioxidant and antimicrobial preparations against test microorganisms.

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In Vitro and In Vivo Antibacterial and Antifungal Screening of Natural Plant Products: Prospective Standardization of Basic Methods

Cited by 9 publications

References 39 publications

Phytochemistry and Preliminary Assessment of the Antibacterial Activity of Chloroform Extract of Amburana cearensis (Allemão) A.C. Sm. against Klebsiella pneumoniae Carbapenemase‐Producing Strains

Phytochemistry and Preliminary Assessment of the Antibacterial Activity of Chloroform Extract of Amburana cearensis (Allemão) A.C. Sm. against Klebsiella pneumoniae Carbapenemase‐Producing Strains

Zoochemical Analyses and In vitro Antimicrobial Activity of Crude Methanolic Extract of Perna viridis

Total Phenolics, Antioxidative and Antimicrobial Activity of Methanol Extracts of Berries of Symphoricarpos Albus (L.) S.F.BLAKE, S. × Chenaultii Rehder and S. Orbiculatus Moench

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