In vitro and in vivo study of the application of volvox spheres to co-culture vehicles in liver tissue engineering

Chang, Siou Han; Huang, Han Hsiang; Kang, Pei; Wu, Yu Chian; Chang, Ming-Huang; Kuo, Shyh Ming

doi:10.1016/j.actbio.2017.09.028

Cited by 17 publications

(13 citation statements)

References 45 publications

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“…Data are presented as the mean ± standard error of the mean (SEM) unless stated otherwise. Results were analyzed using one-way analysis of variance (ANOVA) followed by the Tukey–Kramer multiple comparisons test on SPSS version 17.0 (SPSS Inc., Chicago, IL, USA) to determine whether significant differences existed between the control and experimental groups ( P < 0.05) [38].…”

Section: Methodsmentioning

confidence: 99%

Therapeutic and Protective Effects of Liposomal Encapsulation of Astaxanthin in Mice with Alcoholic Liver Fibrosis

Huang

et al. 2019

IJMS

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Astaxanthin (Asta) has been demonstrated to possess anti-inflammatory, antitumor, and free radical-clearing activities. However, the poor stability and low water solubility of Asta hamper its bioavailability. The objectives of this study were to fabricate Asta-loaded liposomes (Asta-lipo) and investigate the therapeutic effects of Asta-lipo on alcoholic liver fibrosis in mice. The mice were administered with Asta-lipo or liposomes alone prior to a 3-week dose containing 30% alcohol with or without feeding with a second dose of 30% alcohol. The prepared Asta-lipo of 225.0 ± 58.3 nm in diameter, had an encapsulation efficiency of 98%. A slow release profile of 16.2% Asta from Asta-lipo was observed after a 24-h incubation. Restorative actions against alcoholic liver fibrosis were observed after oral administration of Asta-lipo for 4 weeks. Hepatic repair, followed by a second dose of 30% alcohol, suggested that Asta-lipo exerted protective and reparative effects against liver injuries induced by repeated consumption of alcohol. The changes of serum ALT and AST values were principally in consistence with the histopathologic findings. Asta-lipo exerted rapid and direct effects against repeated alcohol-induced liver disease, whereas Asta-lipo given orally could boost recovery from liver injuries obtained due to previous long-term alcohol use. These data demonstrate that Asta-lipo has applicable protective and therapeutic potential to treat alcohol-induced liver diseases.

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Section: Methodsmentioning

confidence: 99%

Therapeutic and Protective Effects of Liposomal Encapsulation of Astaxanthin in Mice with Alcoholic Liver Fibrosis

Huang

et al. 2019

IJMS

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“… 113 , 114 Chen et al 64 recently also reported rescue of acute liver failure in pig models using a multilayer BAL device that contains clinically relevant number of functional hiPSCs-derived hepatic aggregates. Chang et al 18 co-cultured primary rat MSCs and AML12 cell line, mouse liver-derived immortalized hepatocytes, by encapsulating the cells inside collagen/alginate volvox spheres using a high voltage electrostatic field system ( Figure 2(a) ). The hepatic differentiation of MSCs was higher during dynamic cell culture compared to that using static culture.…”

Section: Bioengineering Of Functional Hepatic Constructsmentioning

confidence: 99%

“… ** p < 0.01, *** p < 0.001, compared with the normal group. Adapted with permission from Chang et al 18 …”

Section: Bioengineering Of Functional Hepatic Constructsmentioning

confidence: 99%

Stem cell therapy and tissue engineering strategies using cell aggregates and decellularized scaffolds for the rescue of liver failure

et al. 2021

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Liver failure is a lethal condition with hepatocellular dysfunction, and liver transplantation is presently the only effective treatment. However, due to the limited availability of donors and the potential immune rejection, novel therapeutic strategies are actively sought to restore the normal hepatic architectures and functions, especially for livers with inherited metabolic dysfunctions or chronic diseases. Although the conventional cell therapy has shown promising results, the direct infusion of hepatocytes is hampered by limited hepatocyte sources, poor cell viability, and engraftment. Hence, this review mainly highlights the role of stem cells and progenitors as the alternative cell source and summarizes the potential approaches based on tissue engineering to improve the delivery efficiency of cells. Particularly, the underlying mechanisms for cell therapy using stem cells and progenitors are discussed in two main aspects: paracrine effect and cell differentiation. Moreover, tissue-engineering approaches using cell aggregates and decellularized liver scaffolds for bioengineering of functional hepatic constructs are discussed and compared in terms of the potential to replicate liver physiological structures. In the end, a potentially effective strategy combining the premium advantages of stem cell aggregates and decellularized liver scaffolds is proposed as the future direction of liver tissue engineering and regeneration.

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“…Liver spheroids are a rising model for 3D culture of hepatocytes. These spheroids can mimic the ECM and improve hepatic function in vitro [11,16,75]. The principle of formation of hepatic spheroids is that monodispersed cells are able to change the 3D configuration by self-repolymerizing, with the unavailability of a suitable biomaterial for attachment causing cell-cell attachment into 3D spheroid structures.…”

Section: Liver Spheroidsmentioning

confidence: 99%

Recent advances in the development of in vitro liver models for hepatotoxicity testing

Zhang

Lin

et al. 2021

Bio-des. Manuf.

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Liver injury is a common cause of drug approval withdrawal during drug development, pre-clinical research, and clinical treatment. If not properly treated, patients with severe liver injury can suffer from acute liver failure or even death. Thus, utilization of the convenient in vitro hepatotoxicity assessment model for early detection of drug-induced hepatotoxicity is vital for drug development and safe personalized medication. Biomaterials (e.g., hydrogels, nanofibers, decellularized liver matrix) and bioengineering technologies (e.g., microarrays, micropatterns, 3D printing, and microfluidics) have been applied for in vitro hepatotoxicity assessment models. This review summarizes the structure and functions of the liver as well as the components of in vitro hepatotoxicity assessment models. In addition, it highlights the latest advances in developing hepatotoxicity models with the ultimate goal of further clinical translation.

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In vitro and in vivo study of the application of volvox spheres to co-culture vehicles in liver tissue engineering

Cited by 17 publications

References 45 publications

Therapeutic and Protective Effects of Liposomal Encapsulation of Astaxanthin in Mice with Alcoholic Liver Fibrosis

Therapeutic and Protective Effects of Liposomal Encapsulation of Astaxanthin in Mice with Alcoholic Liver Fibrosis

Stem cell therapy and tissue engineering strategies using cell aggregates and decellularized scaffolds for the rescue of liver failure

Recent advances in the development of in vitro liver models for hepatotoxicity testing

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