In vitro and in vivo antiviral properties of sulfated galactomannans against yellow fever virus (BeH111 strain) and dengue 1 virus (Hawaii strain)

Ono, Lucy; Wollinger, Wagner; Rocco, Iray Maria; Coimbra, Terezinha Lisieux Moraes; Gorin, Philip A.J.; Sierakowski, Maria Rita

doi:10.1016/s0166-3542(03)00175-x

Cited by 126 publications

(92 citation statements)

References 35 publications

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“…Anti-dengue activity of Hippophae rhamnoides leaf extract was demonstrated in DENV-2 infected blood-derived human macrophages (Jain et al 2008). In vitro and in vivo antiviral properties of sulphated galactomannans obtained from the marine alga against DENV have been reported (ono et al 2003(ono et al , Hidari et al 2008. Moreover, enveloped viruses distinct to flavivirus were also inactivated by treatment with the essential oil obtained from medicinal plants.…”

Section: Resultsmentioning

confidence: 99%

Virucidal activity of Colombian Lippia essential oils on dengue virus replication in vitro

Ocazionez

Meneses

Torres

et al. 2010

Mem. Inst. Oswaldo Cruz

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The inhibitory effect of Lippia alba and Lippia citriodora essential oils on dengue virus serotypes replication in vitro was investigated. The cytotoxicity (CC 50 ) was evaluated by the MTT assay and the mode of viral inhibitory effect was investigated with a plaque reduction assay. The virus was treated with the essential oil for 2 h at 37 o C before cell adsorption and experiments were conducted to evaluate inhibition of untreated-virus replication in the presence of oil. Antiviral activity was defined as the concentration of essential oil that caused 50% reduction of the virus plaque number (IC 50 ). L. alba oil resulted in less cytotoxicity than L. citriodora oil (CC 50 : 139.5 vs. 57.6 µg/mL). Virus plaque reduction for all four dengue serotypes was observed by treatment of the virus before adsorption on cell. The IC 50 values for L. alba oil were between 0. 4-32.6 µg/mL and between 1.9-33.7 µg/mL for L. citriodora oil. No viral inhibitory effect was observed by addition of the essential oil after virus adsorption. The inhibitory effect of the essential oil seems to cause direct virus inactivation before adsorption on host cell.Key words: essential oil -antiviral -virucidal activity -dengue virus -Lippia sp.Dengue is an acute illness characterised by the hemorrhagic fever caused by dengue virus (DENV) that is transmitted to humans by Aedes mosquitoes. DENV infection results in a spectrum of disease ranging from a mild febrile illness or dengue fever to a severe disease or dengue hemorrhagic fever/dengue shock syndrome. Among the human arboviral diseases, dengue is responsible for causing most illness and death. It has been estimated that 50-100 million dengue infections occur each year in tropical urban areas around the world with 20,000-25,000 deaths (Gubler 2006).DENV is a member of the Flaviviridae family and it exists as four related serotypes (DENV-1 2, 3 and 4). The virus possesses an icosahedral nucleocapsid core surrounded by a host-derived lipid membrane (envelope), in which the envelope (E) protein and membrane (M) protein are embedded. The Flavivirus enters into the host cell by receptor-mediated endocytosis into coated vesicles, a process in which the E protein plays the major role. The E protein mediates interactions within the virus and the components of the host cell membrane involved in virus adsorption and the E protein interacts with the membrane cell during fusion (Lindenbach & Rice 2007). Compounds that interact with sites on the E protein that binds to structures on the cellular membrane affect the adsorption and fusion process. That interferes There is no antiviral drug for treatment for any of the Flavivirus and an effective vaccine for human use is not yet available to prevent dengue. Consequently, the development of antiviral drugs licensed for treatment of patients remains an urgent need to prevent dengue fatalities. Compounds obtained from traditional medicinal plants and herbs species have been reported to have antiviral activity and a wide variety of active phytochemicals hav...

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Section: Resultsmentioning

confidence: 99%

Virucidal activity of Colombian Lippia essential oils on dengue virus replication in vitro

Ocazionez

Meneses

Torres

et al. 2010

Mem. Inst. Oswaldo Cruz

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“…A temperature of 4 °C has been adopted in the case of cashew gum in order to avoid serious degradation of the polysaccharide. 17 When the same temperature, polysaccharide concentration and solvents were utilized, DS values of 0.50 and 0.62 were obtained by Ono et al, 18 even with a greater sulfation reagent concentration (SR/SU = 31.6).…”

Section: Effect Of Chlorosulfonic Acid Concentration On Yield and Degmentioning

confidence: 87%

“…The sulfation procedure was based on the methodology described by O'Neill 17 and Ono et al 18 with modifications. This method uses chlorosulfonic acid (CSA) as the sulfation agent.…”

Section: Sulfationmentioning

confidence: 99%

“…An increase in the amount of CSA promoted a significant decrease in the sulfation yield. The comparison of the reaction yields of cashew gum and of other gum derivatives obtained using similar methodology was not possible, since this information was not available in either of the reports by O'Neill 17 and Ono et al 18 The apparent yields of more than 100% were reported by Geresh et al 29 in the sulfation of extracellular polysaccharides of red microalgae. In this case, although not specified, the yields were calculated as the mass of sulfated product in relation to the initial mass of polysaccharide.…”

Section: Effect Of Chlorosulfonic Acid Concentration On Yield and Degmentioning

confidence: 99%

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Preparation and characterization of a chemically sulfated cashew gum polysaccharide

Neto¹,

Maciel²,

Cunha³

et al. 2011

J. Braz. Chem. Soc.

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A goma do cajueiro (GC) foi sulfatada em piridina:formamida usando ácido clorossulfônico como reagente. A confirmação da sulfatação foi obtida por espectroscopia no infravermelho com transformada de Fourier (FTIR) pelo aparecimento da frequência de vibração em 1259 cm -1 devido ao estiramento assimétrico S=O. Os graus de sulfatação foram de 0,02, 0,24 e 0,88, determinados pela percentagem de enxofre. Os dados de ressonância magnética nuclear (RMN) 1D e 2D mostram que a sulfatação ocorreu nos carbonos primários do polissacarídeo. Um aumento de no mínimo 4% na viscosidade da solução foi observado após a sulfatação. Análise termogravimétrica (TGA) indica que o derivado é estável até cerca de 200 ºC. A comparação entre os derivados sulfatados e carboximetilados foi feita no intuito de verificar a possibilidade de formação de complexos polieletrolíticos, já realizada com os carboximetilados.Cashew gum (CG) was sulfated in pyridine:formamide using chlorosulfonic acid as the reagent. Confirmation of sulfation was obtained by Fourier transform infrared (FTIR) spectroscopy through the presence of an asymmetrical S=O stretching vibration at 1259 cm -1 . The degrees of substitution were 0.02, 0.24 and 0.88 determined from the sulfur percentage. 1D and 2D nuclear magnetic resonance (NMR) data showed that the sulfation occurred at primary carbons. An increase of at least 4% of the solution viscosity was observed due to sulfation. The thermal gravimetric curves (TGA) indicate that the derivatives are stable up to ca. 200 °C. The sulfated CG is compared to carboxymethylated CG in order to verify the possibility of the use of the former in the preparation of polyelectrolyte complexes; the latter is already being used for this application.

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“…12 Nesse sentido, propõe-se nesse trabalho a extração e caracterização da pectina do melão caipira, bem como sua sulfatação, a fim de que, em estudos posteriores, seja testada quanto à sua capacidade anticoagulante.…”

unclassified

Modificação química da pectina do melão caipira (Cucumis melo var. Acidulus)

Sousa¹,

Ribeiro²,

Santos³

et al. 2017

Quim. Nova

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In vitro and in vivo antiviral properties of sulfated galactomannans against yellow fever virus (BeH111 strain) and dengue 1 virus (Hawaii strain)

Cited by 126 publications

References 35 publications

Virucidal activity of Colombian Lippia essential oils on dengue virus replication in vitro

Virucidal activity of Colombian Lippia essential oils on dengue virus replication in vitro

Preparation and characterization of a chemically sulfated cashew gum polysaccharide

Modificação química da pectina do melão caipira (Cucumis melo var. Acidulus)

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