In vitro and in vivo antitumor effects of Peanut agglutinin through induction of apoptotic and autophagic cell death

Mukhopadhyay, Subhadip; Panda, Prashanta Kumar; Behera, Birendra; Das, Chandan; Hassan, Md. Khurshidul; Das, Durgesh Nandini; Sinha, Niharika; Bissoyi, Akalabya; Pramanik, Krishna; Maiti, Tapas K.; Bhutia, Sujit K.

doi:10.1016/j.fct.2013.11.046

Cited by 43 publications

(39 citation statements)

References 34 publications

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“…We customarily checked the autophagic level by performing acridine orange staining as noted [13]. A relative increase in red fluorescence intensity signal signifying late phase autophagic vesicles was shown as in Ref.…”

Section: 11 Measurement Of Autophagymentioning

confidence: 99%

“…[13]. Again to detect the level of mitochondrial superoxides, Mito-SOX staining was used for live cell imaging and quantified using Image J as noted in section 2.9 [21].…”

Section: 12 Measurement Of Reactive Oxygen Speciesmentioning

confidence: 99%

“…After centrifuging at 12,000g ( at 4 °C) the supernatant was subjected to horizontal agarose electrophoresis. Ethidium bromide-stained DNA was visualized in a Bio-Rad gel documentation system using the Quantity One 1-D analysis software (version 4.6.9, Bio-Rad Laboratories, Inc., Hercules, CA, USA) [13].…”

Section: 6 Dna Fragmentation Assay In Hela Cellsmentioning

confidence: 99%

“…Nuclear staining using DAPI was performed according to the method previously described [13]. In short, HeLa cells with/without CDDP were smeared on a glass slide, fixed with 3.7% paraformaldehyde for 15 min, permeabilized, and stained with 1 μg/ml DAPI for 5 min at 37 °C.…”

Section: 5 Dapi (4′6-diamidino-2-phenylindole Dihydrochloride) Stmentioning

confidence: 99%

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Autophagy protein Ulk1 promotes mitochondrial apoptosis through reactive oxygen species

Mukhopadhyay

Das

Panda

et al. 2015

Free Radical Biology and Medicine

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Section: 11 Measurement Of Autophagymentioning

confidence: 99%

“…[13]. Again to detect the level of mitochondrial superoxides, Mito-SOX staining was used for live cell imaging and quantified using Image J as noted in section 2.9 [21].…”

Section: 12 Measurement Of Reactive Oxygen Speciesmentioning

confidence: 99%

Section: 6 Dna Fragmentation Assay In Hela Cellsmentioning

confidence: 99%

Section: 5 Dapi (4′6-diamidino-2-phenylindole Dihydrochloride) Stmentioning

confidence: 99%

See 2 more Smart Citations

Autophagy protein Ulk1 promotes mitochondrial apoptosis through reactive oxygen species

Mukhopadhyay

Das

Panda

et al. 2015

Free Radical Biology and Medicine

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“…DNA fragmentation assay in AGG-treated HepG2 cells For the DNA fragmentation assay, a non-enzymatic method was adopted [16] . Briefly, two million HepG2 cells were seeded onto a 60-mm Petri dish and treated with different concentrations of AGG.…”

Section: Clonogenic Survival Determination In Hepg2 Cellsmentioning

confidence: 99%

Abrus agglutinin suppresses human hepatocellular carcinoma in vitro and in vivo by inducing caspase-mediated cell death

et al. 2014

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Aim: Abrus agglutinin (AGG) from the seeds of Indian medicinal plant Abrus precatorius belongs to the class II ribosome inactivating protein family. In this study we investigated the anticancer effects of AGG against human hepatocellular carcinoma in vitro and in vivo. Methods: Cell proliferation, DNA fragmentation, Annexin V binding, immunocytofluorescence, Western blotting, caspase activity assays and luciferase assays were performed to evaluate AGG in human liver cancer cells HepG2. Immunohistochemical staining and TUNEL expression were studied in tumor samples of HepG2-xenografted nude mice.

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A natural product enhances apoptosis via mitochondria/caspase‐mediated pathway in HeLa cells

Fan

Zhang

Liu

et al. 2019

J of Cellular Biochemistry

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Cervical cancer is the fourth most lethal human malignancy and the leading cause of death among females around the world. Many antitumor agents have microbial origins. 5′‐epi‐SPA‐6952A is a new 24‐membered macrolide isolated from the cultured broth of Streptomyces diastatochromogenes. Therefore, we studied the activity and molecular mechanism of 5′‐epi‐SPA‐6952A in human cervical carcinoma HeLa cell. The results showed that 5′‐epi‐SPA‐6952A significantly inhibited cell proliferation and migration. In addition, 5′‐epi‐SPA‐6952A obviously increased the production of intracellular reactive oxygen species and DNA damage in HeLa cells. Moreover, nuclear shrinkage of cells, decrease in mitochondrial membrane potential, and upregulation of Bax/Bcl‐2 ratio resulted in the release of cytochrome c, and activation of caspase‐9/3 was observed in HeLa cells treated with 5′‐epi‐SPA‐6952A, which means it enhanced the intrinsic mitochondrial apoptosis. Besides, DNA‐damage associated proteins poly (ADP‐ribose) polymerase (PARP) and p53 were also studied, and the expressions of cleaved‐PARP and p53 were drastically increased in HeLa cells treated with 5′‐epi‐SPA‐6952A. Furthermore, we confirmed that 5′‐epi‐SPA‐6952A affected the survival of HeLa cells by blocking cell cycle progression in the G1 phase. Taken together, the results shows that 5′‐epi‐SPA‐6952A significantly inhibited HeLa cells proliferation via intrinsic mitochondrial apoptosis, cell cycle arrest, and blocking cell migration.

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In vitro and in vivo antitumor effects of Peanut agglutinin through induction of apoptotic and autophagic cell death

Cited by 43 publications

References 34 publications

Autophagy protein Ulk1 promotes mitochondrial apoptosis through reactive oxygen species

Autophagy protein Ulk1 promotes mitochondrial apoptosis through reactive oxygen species

Abrus agglutinin suppresses human hepatocellular carcinoma in vitro and in vivo by inducing caspase-mediated cell death

A natural product enhances apoptosis via mitochondria/caspase‐mediated pathway in HeLa cells

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