In vitro and In vivo Anti-inflammatory Activities of Coptosapelta flavescens Korth Root’s Methanol Extract

Abstract

Coptosapelta flavescens Korth is a liana plant of the Rubiaceae family known in East Kalimantan as Akar Tambolekar or Akar Merung. Its anti-inflammatory activity has not been scientifically proven in vitro or in vivo. The current study aimed to prove the anti-inflammatory activity of C. flavescens root (CFR) methanol extract in vitro and in vivo. The anti-inflammatory activity of CFR methanol extract was evaluated in vitro by examining its ability to stabilize the membrane of hypotonicity-induced red blood cells (RBC), and in vivo study showed the effect on carrageenaninduced inflammation on the left leg of Wistar rats following its oral administration at 1 hour before, during, and after carrageenan induction. Result of in vitro study revealed that the EC50 of CFR methanol extract was 1.905 ± 0.119 mg/ml, lower than that of indomethacin (10.288 ± 0.212 mg/ml). In vivo study showed that CFR methanol extract at doses of 600 and 1200 mg/kg administered 1 hour before and during carrageenan induction exhibited antiinflammatory activity, but only the CFR methanol extract showed anti-inflammatory activity at a dose of 1200 mg/ kg when administered 1 hour after carrageenan induction. CFR methanol extract exhibited anti-inflammatory activity both in vitro and in vivo.

Antioxidant, Anti-Inflammatory, Acute Oral Toxicity, and Qualitative Phytochemistry of The Aqueous Root Extract of Launaea cornuta (Hochst. Ex Oliv. & Hiern.)

“…46 Additionally, various commonly used NSAIDs work by inhibiting the activity of these enzymes or preventing lysis of the lysosomal membranes to block release of the proteases. 55,56 We noted that the aqueous root extract of L. cornuta exerted significant membrane stabilization activity in hypotonic and heat induced HRBC haemolysis. Our findings corroborate those observed by Leelaprakash et al 51 Our study suggests that the aqueous root extract of L. cornuta possesses phytochemicals that stabilise erythrocyte membranes and prevent lysis upon exposure to osmotic or heat stress.…”

“…46 Additionally, various commonly used NSAIDs work by inhibiting the activity of these enzymes or preventing lysis of the lysosomal membranes to block release of the proteases. 55 , 56 …”

“…Furthermore, steroids have been reported to inhibit the secretion of anti-inflammatory mediators. 56 Therefore, these compounds may have played a critical role, either solely, or synergistically, in conferring the antioxidant and anti-inflammatory efficacy of the aqueous root extract of L. cornuta. Therefore, the findings of this study partly validate the healing claims against inflammatory-associated disease, as reported in traditional medicine.…”

Anti-Inflammatory Activity of Rhaphidophora pinnata (L.F) Schott Leaf Extract

“…Catechin and epigallocatechin gallate from green tea may inhibit COX2 expression induced by 12-O-tetradecanoylphorbol-13-acetate, tumor promoters in rat skin, and COX activity in macrophages induced by lipopolysaccharides [9], [10]. Quercetin-3-methoxy-40-glucosyl-7-glucoside from Maytenus heterophylla was effective as antiinflammatory against carrageenan-induced paw edema in rats [11]. The previous study showed that R. pinnata contain flavonoids, so it is possible to develop it as anti-inflammatory.…”

UJI AKTIVITAS ANTIINFLAMASI EKSTRAK ETANOL DAUN SERAI WANGI (Cymbopogon nardus L.) SECARA IN VITRO DENGAN METODE HRBC (Human Red Blood Cell)

“…Larutan uji terdiri dari 1 ml dapar fosfat pH 7,4 (0,15 M), 0,5 ml suspensi sel darah merah, 2 ml hiposalin dan 0,5 ml larutan sampel pada berbagai konsentrasi (Kosala et al 2018). b. Pembuatan larutan kontol positif Larutan terdiri dari campuran dapar fosfat pH 7,4 (0,15 M) sebanyak 1 ml, suspensi sel darah merah sebanyak 0,5 ml, hiposalin sebanyak 2 ml dan larutan aspirin berbagai konsentrasi sebanyak 0,5 ml (Kosala et al 2018). c. Pembuatan larutan kontrol negatif Larutan kontrol negatif terdiri 1 ml dapar fosfat pH 7,4 (0,15 M), 0,5 ml suspensi sel darah merah, 2 ml hiposalin dan 1 ml larutan isosalin (Kosala et al 2018)…”