In Vitro and In Vivo Isolation and Characterization of Duvenhage Virus

Koraka, Penelope; Martina, Benedict; Roose, Jouke M.; Thiel, Pieter-Paul A. M. van; Amerongen, Geert van; Kuiken, Thijs; Osterhaus, Albert D. M. E.

doi:10.1371/journal.ppat.1002682

Cited by 15 publications

(16 citation statements)

References 30 publications

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“…Several passages affect the pathogenicity of the virus on IM route of inoculation resulting only pathogenic to cell lines and intracerebral route of inoculation. In studies done by Koraka and his colleague, pathogenicity of lyssaviruses known to be invasive when isolated from wild and inoculated on mice before adaptation by several passages to cell lines and mice brains (Koraka et al, 2012). Therefore, decline in pathogenicity of the virus to intramuscular route of inoculation may indicate that the viruses have adapted to grow on mice brain and cell lines.…”

Section: Discussionmentioning

confidence: 99%

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Adaptation of Local Rabies Virus Isolates to High Growth Titer and Determination of Pathogenicity to Develop Canine Vaccine in Ethiopia

Mengesha¹

2014

J Vaccines Vaccin

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Rabies is a zoonotic viral disease which causes acute encephalitis in humans and animals. The case is most severe in developing countries where cell culture derived anti-rabies vaccines are unaffordable or the available nervous tissue-derived vaccines are of questionable immunogenicity and may cause neurological complications. The aim of this research was to adapt local rabies virus isolates on BHK-21 and to study pathogenicity to intramuscular route of inoculation for canine vaccine development. The viruses were isolated from rabid dogs' brain and human saliva, and adapted to Swiss albino mice brain and cell lines by several blind passages. By titration, a minimum of 10 6.5 TCID 50 /ml (in vitro) and 10 4.5 MICLD 50 /0.03 ml (in vivo) virus titer were obtained. For pathogenicity study, mice were inoculated intramuscularly with 250MICLD 50 /0.1 ml of each adapted virus isolate and observed for 45 days. Only two virus isolates, human origin sululta (HOS) and dog origin (DO) caused 12.5% death. This can show the phylogroup origin of the viruses indicating phylogroup I origin of these virus isolates with decline in virulence. Decline in pathogenicity may be due to adaptation of the viruses to mice brain and cell lines to increase virus infectivity titer. Generally, the exact genetic relationship with fixed rabies virus strain should be studied by molecular techniques and canine anti-rabies vaccine develops from locally isolated viruses.

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Section: Discussionmentioning

confidence: 99%

“…Viruses that are more closely related to rabies virus can be neutralized, at least to some extent, by antibodies to rabies virus [9]. Viruses that are far from rabies virus in their glycoprotein genetic makeup cannot be neutralized by antibody raised to classical rabies virus and can affect efficacy of the vaccine [9][10][11][12][13].…”

Section: Introductionmentioning

confidence: 99%

Adaptation of Local Rabies Virus Isolates to High Growth Titer and Determination of Pathogenicity to Develop Canine Vaccine in Ethiopia

Mengesha¹

2014

J Vaccines Vaccin

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“…In addition, no cytopathic effect was observed which might be related to the relatively low number of infected cells in vitro . The absence of visible cytopathic effect does not exclude that this virus is indeed pathogenic in vivo, since many viruses do not cause visible cytopathic effect but are still pathogenic [31,32]. In addition to the brain, Seal parvovirus DNA was detected in various other tissues, including the lung, the liver and the spleen.…”

Section: Discussionmentioning

confidence: 99%

Novel B19-Like Parvovirus in the Brain of a Harbor Seal

Bodewes

García²,

Wiersma

et al. 2013

PLoS ONE

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Using random PCR in combination with next-generation sequencing, a novel parvovirus was detected in the brain of a young harbor seal (Phoca vitulina) with chronic non-suppurative meningo-encephalitis that was rehabilitated at the Seal Rehabilitation and Research Centre (SRRC) in the Netherlands. In addition, two novel viruses belonging to the family Anelloviridae were detected in the lungs of this animal. Phylogenetic analysis of the coding sequence of the novel parvovirus, tentatively called Seal parvovirus, indicated that this virus belonged to the genus Erythrovirus, to which human parvovirus B19 also belongs. Although no other seals with similar signs were rehabilitated in SRRC in recent years, a prevalence study of tissues of seals from the same area collected in the period 2008-2012 indicated that the Seal parvovirus has circulated in the harbor seal population at least since 2008. The presence of the Seal parvovirus in the brain was confirmed by real-time PCR and in vitro replication. Using in situ hybridization, we showed for the first time that a parvovirus of the genus Erythrovirus was present in the Virchow-Robin space and in cerebral parenchyma adjacent to the meninges. These findings showed that a parvovirus of the genus Erythrovirus can be involved in central nervous system infection and inflammation, as has also been suspected but not proven for human parvovirus B19 infection.

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“…2). Recently, Koraka et al (2012) reported that DUVV shows similar peripheral pathogenicity in mice to that of the RABV fixed strain, but is less pathogenic than an RABV street strain. Moreover, LBV and MOKV are less pathogenic in mice after intramuscular infection (Badrane et al 2001).…”

Section: Rabies Virus P Targets To Ikkementioning

confidence: 99%

Contribution of the interaction between the rabies virus P protein and I-kappa B kinase ϵ to the inhibition of type I IFN induction signalling

Masatani

Ozawa

Yamada

et al. 2016

Journal of General Virology

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The P protein of rabies virus (RABV) is known to interfere with the phosphorylation of the host IFN regulatory factor 3 (IRF-3) and to consequently inhibit type I IFN induction. Previous studies, however, have only tested P proteins from laboratory-adapted fixed virus strains, and to the best of our knowledge there is no report about the effect of P proteins from street RABV strains or other lyssaviruses on the IRF-3-mediated type I IFN induction system. In this study, we evaluated the inhibitory effect of P proteins from several RABV strains, including fixed and street virus strains and other lyssaviruses (Lagos bat, Mokola and Duvenhage viruses), on IRF-3 signalling. All P proteins tested inhibited retinoic acid-inducible gene-1 (RIG-I)-and TANK binding kinase 1 (TBK1)-mediated IRF-3-dependent IFN-b promoter activities. On the other hand, the P proteins from the RABV street strains 1088 and HCM-9, but not from fixed strains Nishigahara (Ni) and CVS-11 and other lyssaviruses tested, significantly inhibited I-kappa B kinase e (IKKe)-inducible IRF-3-dependent IFN-b promoter activity. Importantly, we revealed that the P proteins from the 1088 and HCM-9 strains, but not from the remaining viruses, interacted with IKKe. By using expression plasmids encoding chimeric P proteins from the 1088 strain and Ni strain, we found that the C-terminal region of the P protein is important for the interaction with IKKe. These findings suggest that the P protein of RABV street strains may contribute to efficient evasion of host innate immunity.

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In Vitro and In Vivo Isolation and Characterization of Duvenhage Virus

Cited by 15 publications

References 30 publications

Adaptation of Local Rabies Virus Isolates to High Growth Titer and Determination of Pathogenicity to Develop Canine Vaccine in Ethiopia

Adaptation of Local Rabies Virus Isolates to High Growth Titer and Determination of Pathogenicity to Develop Canine Vaccine in Ethiopia

Novel B19-Like Parvovirus in the Brain of a Harbor Seal

Contribution of the interaction between the rabies virus P protein and I-kappa B kinase ϵ to the inhibition of type I IFN induction signalling

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