In vitro and in vivo differential expression of rainbow trout (Oncorhynchus mykiss) Mx isoforms in response to viral haemorrhagic septicaemia virus (VHSV) G gene, poly I:C and VHSV

Tafalla, Carolina; Chico, V.; Pérez, Luis; Coll, Julio; Estepa, A.

doi:10.1016/j.fsi.2006.10.009

Cited by 77 publications

(47 citation statements)

References 49 publications

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“…Thus, viral factors involved in the control of IFN levels may determine the virulence of VHSV. VHSV G has been reported to induce an IFN response (Boudinot et al 2004, Tafalla et al 2007 and play a decisive role in determining the virulence of VHSV (Béarzotti et al 1995, Stone et al 1997. The NV of IHNV, a fish rhabdovirus, has been found to suppress the host IFN response and support viral growth (Choi et al 2011).…”

Section: Resultsmentioning

confidence: 99%

“…However, it is not clear how the single amino acid substitution in the G protein affects VHSV virulence. As the VHSV G protein is involved in the induction of the host IFN response (Boudinot et al 2004, Tafalla et al 2007, a substitution in the VHSV G protein might affect this induction and thus virulence. Alternatively, the change in the G protein sequence may affect the efficiency of initial entry of VHSV.…”

Section: Resultsmentioning

confidence: 99%

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Genetically similar VHSV isolates are differentially virulent in olive flounder Paralichthys olivaceus

Cho¹,

Lee²,

Moon³

et al. 2012

Dis. Aquat. Org.

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Two viral hemorrhagic septicemia virus (VHSV) isolates, VHSV-KR-CJA and VHSV-KR-YGH, were isolated from viral hemorrhagic septicemia disease outbreaks in flounder farms in South Korea. The VHSV-KR-CJA isolate was isolated from a flounder farm with high mortality (80%), while the VHSV-KR-YGH isolate was isolated from a flounder farm with low mortality (15%), suggesting that these isolates differ in virulence. The virulence of these isolates was evaluated in juvenile flounder via intraperitoneal injection. Consistent with their virulence in the field, mortality data revealed that the VHSV-KR-CJA isolate was highly pathogenic (cumulative mortality of 80%), while the VHSV-KR-YGH isolate was less pathogenic in flounder (cumulative mortality of 20%). To characterize the genotypes of these viruses, the full open reading frames (ORFs) encoding nucleoprotein N, phosphoprotein P, matrix protein M, glycoprotein G, nonstructural viral protein NV, and polymerase L of these viruses were sequenced and analyzed. Sequence analysis revealed that both isolates are genetically very similar (identical amino acid sequences for P, M, NV, and L and > 99.7 and 99.8% amino acid sequence identity for N and G, respectively). Phylogenetic analysis indicated that both of these viruses belong to the Genotype IVa group, suggesting that they originated from a common ancestral virus. The low pathogenicity VHSV strain may potentially evolve to become a more pathogenic strain through only a few nucleotide substitutions. Further functional analyses of mutations in VHSV genes are necessary to identify factors that determine VHSV pathogenicity in flounder. KEY WORDS: Viral hemorrhagic septicemia virus · VHSV · Virulence · Flounder Resale or republication not permitted without written consent of the publisherDis Aquat Org 101: [105][106][107][108][109][110][111][112][113][114] 2012 Phylogenetic analyses of the N-and G-encoding genes of VHSV have identified 4 main genotypes (I to IV), with several subgroups within Genotypes I (minimum Ia to Ie) and IV (IVa and IVb) (Snow et al. 1999, Einer-Jensen et al. 2004, Lumsden et al. 2007). These genotypes reflect a largely geographic rather than a species-specific distribution. Genotype I includes a wide range of viruses originating from freshwater rainbow trout farms in continental Europe (Thiéry et al. 2002, Einer-Jensen et al. 2004) and a large number of isolates originating from marine species in the Baltic Sea, Skagerrak, Kattegat, and English Channel (Dixon et al. 1997, Einer-Jensen et al. 2004, Snow et al. 2004. Genotype II includes a limited number of VHSV isolates recovered from the Baltic Sea (Snow et al. 2004). Genotype III includes isolates from the North Sea (Einer-Jensen et al. 2004, Snow et al. 2004, North Atlantic (López-Vázquez et al. 2006), and from seawater-reared rainbow trout in western Norway (Dale et al. 2009). Genotype IV consists of viruses from the Pacific coast and Atlantic coast of North America as well as isolates from the Great Lakes region of North America and from ...

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Genetically similar VHSV isolates are differentially virulent in olive flounder Paralichthys olivaceus

Cho¹,

Lee²,

Moon³

et al. 2012

Dis. Aquat. Org.

View full text Add to dashboard Cite

show abstract

“…The latter might be targets of viral inhibitory signals early after infection (Encinas et al, 2010). These results showed that 2 days after infection-by-immersion, VHSV had not yet caused an strong response from zebrafish internal organs, which contrasts with reports in other fish at later times after infection-by-injection (such as ifn1, mx, il1b, tnfa, etc) (Acosta et al, 2006;Samuel, 2001;Tafalla et al, 2007;Tafalla et al, 2005) or infection-by-immersion (Jorgensen et al, 2011;Zhang et al, 2009). …”

Section: Microarrays In the Study Of The Vhsv/zebrafish Modelmentioning

confidence: 71%

“…As established by quantitative RT-qPCR before the advent of microarrays, 4 to 8 days after DNA vaccination by intramuscular injection, gene expression by fish haematopoietic organs showed an increase in interferon-inducible mx (Acosta et al, 2005;Boudinot et al, 1998;McLauchlan et al, 2003;Purcell et al, 2004;Robertsen, 2008;Tafalla et al, 2007), virallyinduced genes (Vig) (Boudinot et al, 1999;Boudinot et al, 2001) and mhc and tcr genes (Takano et al, 2004). 1 cDNA HRV-infected Leukocytes (Aoki et al, 1999) (Nam et al, 2000) European flounder…”

Section: Introductionmentioning

confidence: 99%

Use of Microarray Technology to Improve DNA Vaccines in Fish Aquaculture - The Rhabdoviral Model

Encinas¹,

Gómez-Casado²,

Estepa³

et al. 2012

Health and Environment in Aquaculture

Self Cite

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“…Atlantic salmon Mx1 inhibits an influenza-like virus in fish, called infectious salmon anaemia virus (ISAV) and also infectious pancreatic necrosis virus (IPNV), an aquatic birnavirus [14,15]. Rainbow trout Mx can be induced by chum salmon reovirus (CSR), Poly I:C, or viral haemorrhagic septicaemia virus (VHSV) in trout monocyte/macrophage and fibroblast cell lines [16,17]. All the three Mx isoforms from grouper have the efficiency of reducing the titer of virus 10-to 100-fold in GB3 cells [18,19].…”

Section: Introductionmentioning

confidence: 99%

Enhanced grass carp reovirus resistance of Mx-transgenic rare minnow (Gobiocypris rarus)

Yang

Zhang

et al. 2009

Fish & Shellfish Immunology

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In vitro and in vivo differential expression of rainbow trout (Oncorhynchus mykiss) Mx isoforms in response to viral haemorrhagic septicaemia virus (VHSV) G gene, poly I:C and VHSV

Cited by 77 publications

References 49 publications

Genetically similar VHSV isolates are differentially virulent in olive flounder Paralichthys olivaceus

Genetically similar VHSV isolates are differentially virulent in olive flounder Paralichthys olivaceus

Use of Microarray Technology to Improve DNA Vaccines in Fish Aquaculture - The Rhabdoviral Model

Enhanced grass carp reovirus resistance of Mx-transgenic rare minnow (Gobiocypris rarus)

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