In vitro and in vivo potential of a blend of essential oil compounds to improve rumen fermentation and performance of dairy cows

Joch, Miroslav; Kudrna, V.; Hakl, J.; Božík, Matěj; Homolka, P.; Illek, J.; Tyrolová, Yvona; Výborná, Alena

doi:10.1016/j.anifeedsci.2019.03.009

Cited by 29 publications

(23 citation statements)

References 42 publications

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“…In the study by Joch et al [42], bornyl acetate was defined as the most promising compound among the 11 EO compounds evaluated, which included different chemical groups, such as alcohols (linalool), aldehydes (citral), ethers (1,4 -cineol), hydrocarbons (p-cymene, limonene, α, and β pinene, γ terpinen), and phenolic compounds (carvacrol, eugenol). Bornyl acetate at 480 mg/L demonstrated the most beneficial effects on rumen fermentation when expressed as methane production per VFA production [44]. Although the dose was significantly higher than that of the present study, the lower relative activity of bornyl acetate at such high doses may be due to the fact that when added directly to the incubation medium and considering its lipophilic nature, the dispersion in the medium may have been smaller, thus reducing the toxicity of this compound to ruminal microorganisms.…”

Section: Discussionmentioning

confidence: 99%

“…The difficulty of translating the dose of one system to the following step might be a limitation scale-up in experimental complexity. For instance, Joch et al [44] compared the effect of EO in vitro and in vivo, and observed that effects on fermentation in vitro were manifested at approximately 30 times higher concentrations than in in vivo conditions.…”

Section: Discussionmentioning

confidence: 99%

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The Reduction of Methane Production in the In Vitro Ruminal Fermentation of Different Substrates is Linked with the Chemical Composition of the Essential Oil

García

Colombatto

Brunetti

et al. 2020

Animals

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There is interest in identifying natural products capable of manipulating rumen microbial activity to develop new feed additives for ruminant nutrition as a strategy to reduce methane. Two trials were performed using the in vitro gas production technique to evaluate the interaction of substrate (n = 5) and additive (n = 6, increasing doses: 0, 0.3, 3, 30, and 300 µL/L of essential oils—EO—of Lippia turbinata or Tagetes minuta, and monensin at 1.87 mg/L). The two EO utilized were selected because they differ markedly in their chemical composition, especially in the proportion of oxygenated compounds. For both EO, the interaction between the substrate and additive was significant for all variables; however, the interaction behaved differently for the two EO. Within each substrate, the response was dose-dependent, without effects at a low level of EO and a negative outcome at the highest dose. The intermediate dose (30 µL/L) inhibited methane with a slight reduction on substrate digestibility, with L. turbinata being more effective than T. minuta. It is concluded that the effectiveness of the EO to reduce methane production depends on interactions between the substrate that is fermented and the additive dose that generates different characteristics within the incubation medium (e.g., pH); and thus, the chemical nature of the compounds of the EO modulates the magnitude of this response.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

The Reduction of Methane Production in the In Vitro Ruminal Fermentation of Different Substrates is Linked with the Chemical Composition of the Essential Oil

García

Colombatto

Brunetti

et al. 2020

Animals

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“…The in vitro batch incubations were conducted as described by Joch et al (2019) with modifications. These modifications (reducing the amount of substrate and volume of culture fluid) were made to ensure headspace pressure in bottles to be not more than 483 hPa as recommended by Theodorou et al (1994).…”

Section: In Vitro Incubationmentioning

confidence: 99%

“…Then, 5 mL of fermentation gas was transferred to a tube filled with distilled water by displacement. Methane concentration in fermentation gas was determined by gas chromatography (Joch et al, 2019), calibration and calculations were performed as described previously (López and Newbold, 2007).…”

Section: Sampling and Analysismentioning

confidence: 99%

Inhibition of in Vitro Rumen Methane Production by Three Statins

Joch

Vadroňová

Výborná

et al. 2022

Annals of Animal Science

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The aim of this study was to evaluate the effects of increasing concentrations of three pure statins on in vitro methane production and rumen fermentation. The effects of atorvastatin, rosuvastatin and simvastatin at three concentrations (1, 10, and 100 mg/L of culture fluid) were evaluated using in vitro 24 h batch incubation of buffered rumen fluid with a 70:30 forage:concentrate substrate. All statins tested demonstrated the ability to reduce methanogenesis. Methane inhibition potential was decreasing in the following order: simvastatin > atorvastatin > rosuvastatin. Methane production was reduced (p < 0.05) by simvastatin at 10 mg/L (by 9.3%) and by atorvastatin at 100 mg/L (by 13.2%) without compromising fermentation and feed digestibility. Simvastatin at 100 mg/L decreased methane production by 26.2%, however, net production of volatile fatty acids (nVFA) was also reduced (p < 0.05). The only effect of rosuvastatin was a slight reduction (p < 0.05) of methane proportion at 10 and 100 mg/L. Simvastatin and atorvastatin at 100 mg/L increased (p < 0.05) relative proportion of propionate at the expense of acetate and butyrate. Ammonia-N concentrations were not affected (p > 0.05) by statins. The current study demonstrated that selected statins could selectively decrease methane production. The effects of statins on methanogenesis and overall rumen fermentation vary depending on statin type and concentration. Hydrophobic statins, such as simvastatin and atorvastatin, seem to be more effective compared to the hydrophilic statins, such as rosuvastatin.

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“…Among these alternatives, essential oils and their compounds have attracted much attention because of their antimicrobial properties that may high potential for binding to proteins (Hodaj-Çeliku et al, 2017;Abudunia et al, 2017). Therefore, these compounds modulate rumen fermentation (Joch et al, 2019;Garcia et al, 2020). Essential oils from aromatic and medicinal plants have been shown to have selective antimicrobial properties and also have a are able to affect the microbiology and protein breakdown in silage (Besharati, Palangi, Moaddab, Nemati, Pliego, & Salem, 2020).…”

Section: Introductionmentioning

confidence: 99%

Optimization of dietary lemon seed essential oil to enhance alfalfa silage chemical composition and in vitro degradability

Besharati

Palangi

Niazifar

et al. 2021

Semina: Ciênc. Agrár.

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Purpose of the experiment was to evaluate the effect of lemon-seed essential oils on chemical composition and in vitro degradability of alfalfa silage. Treatments were alfalfa silage with no additive (control) or treated with 60 ml/kg DM of lemon-seed (C60), of lemon-seed (C120) essential oils and equal mixed of them (M60). Whole plant alfalfa silage was ensiled for 60 d in triplicate laboratory scale tubes. Dry matter, organic matter, crude protein, insoluble fiber in acidic and neutral detergent, water soluble carbohydrate, pH, were measured with 3 replicates by in vitro gas production method. Dry matter (DM) content was greater for leo60 than control. Compared with control, neutral detergent fiber (NDF) concentration was decreased in leo120. Addition of essential oils and their combination to the silage significantly decreased (p<.0001) silage pH compared with untreated silage interestingly increased for all the silages containing essential oil compared with untreated silage. Addition of lemon-seed essential to alfalfa silage decreased the rate of disappearance of organic matter and dry matter in all treatments compared to the control treatment. The degradability potential of alfalfa silage has increased in treatments containing lemon-seed essential oil (60 ml / kg DM) which is significantly different from the control. In general, the obtained data show the positive effect of lemon-seed essential oil on the quality of alfalfa silage and its fermentation properties.

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In vitro and in vivo potential of a blend of essential oil compounds to improve rumen fermentation and performance of dairy cows

Cited by 29 publications

References 42 publications

The Reduction of Methane Production in the In Vitro Ruminal Fermentation of Different Substrates is Linked with the Chemical Composition of the Essential Oil

The Reduction of Methane Production in the In Vitro Ruminal Fermentation of Different Substrates is Linked with the Chemical Composition of the Essential Oil

Inhibition of in Vitro Rumen Methane Production by Three Statins

Optimization of dietary lemon seed essential oil to enhance alfalfa silage chemical composition and in vitro degradability

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