In vitro and in vivo investigation of PLA/PCL scaffold coated with metformin-loaded gelatin nanocarriers in regeneration of critical-sized bone defects

Shahrezaee, Mostafa; Salehi, Majid; Keshtkari, Sara; Oryan, Ahmad; Kamali, Amir; Shekarchi, Babak

doi:10.1016/j.nano.2018.06.007

Cited by 101 publications

(63 citation statements)

References 46 publications

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“…As previously proposed, biomaterials functionalized with metformin exhibit an advantage in osteogenic differentiation and bone regeneration . In line with previous studies, the results of the current study showed that metformin enhanced expression of osteoblast markers and calcium deposition in hPDLSCs exposed to tHA, suggesting that metformin‐coated tHA may improve the bone‐regeneration effect of tHA.…”

Section: Discussionsupporting

confidence: 91%

Effect of metformin on human periodontal ligament stem cells cultured with polydopamine‐templated hydroxyapatite

Zhan

Gao

Zhou

et al. 2019

European J Oral Sciences

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Polydopamine‐templated hydroxyapatite (tHA) is a type of nano‐biomaterial that can promote osteogenesis in bone tissue engineering. However, high concentrations of tHA stimulate production of reactive oxygen species (ROS), resulting in cell injury and apoptosis. Metformin has been demonstrated to activate the adenosine monophosphate‐activated protein kinase (AMPK) signaling pathway, which induces autophagy and decreases ROS production to prevent apoptosis. The present study was performed to investigate the potential application of tHA in combination with metformin in periodontal bone tissue engineering. Human periodontal ligament stem cells (hPDLSCs) were exposed to tHA in the presence or absence of metformin, and cytocompatibility and osteogenesis were detected by related assays. Additionally, the autophagy signaling pathway was analyzed by western blotting. Polydopamine‐templated hydroxyapatite, in combination with metformin, substantially reduced ROS production and apoptosis, and enhanced proliferation and osteogenic differentiation of hPDLSCs. Enhanced levels of microtubule‐associated protein 1 light chain 3 II and Beclin‐1 were observed after exposure to tHA plus metformin. Expression of phosphorylated AMPK was increased and that of phosphorylated mammalian target of rapamycin (mTOR) was decreased after exposure to tHA plus metformin. Taken together, our results demonstrate that tHA, combined with metformin, increases the viability of hPDLSCs via the AMPK/mTOR signaling pathway by regulating autophagy and further improving the osteogenic effect.

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Section: Discussionsupporting

confidence: 91%

Effect of metformin on human periodontal ligament stem cells cultured with polydopamine‐templated hydroxyapatite

Zhan

Gao

Zhou

et al. 2019

European J Oral Sciences

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“…As with other bio metals, magnesium is investigated to promote the cell's response on the surface. The healing rate of bone is critical [3], and, for successful bone regeneration, cell viability is highly significant [4]. Cell adhesion is unlikely to occur directly on the implant.…”

Section: Introductionmentioning

confidence: 99%

Investigation of the Oxidation Mechanism of Dopamine Functionalization in an AZ31 Magnesium Alloy for Biomedical Applications

et al. 2019

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Implant design and functionalization are under significant investigation for their ability to enhance bone-implant grafting and, thus, to provide mechanical stability for the device during the healing process. In this area, biomimetic functionalizing polymers like dopamine have been proven to be able to improve the biocompatibility of the material. In this work, the dip coating of dopamine on the surface of the magnesium alloy AZ31 is investigated to determine the effects of oxygen on the functionalization of the material. Two different conditions are applied during the dip coating process: (1) The absence of oxygen in the solution and (2) continuous oxygenation of the solution. Energy dispersive spectroscopy (EDS) and Fourier transform infrared spectroscopy (FTIR) are used to analyze the composition of the formed layers, and the deposition rate on the substrate is determined by molecular dynamic simulation. Electrochemical analysis and cell cultivation are performed to determine the corrosion resistance and cell’s behavior, respectively. The high oxygen concentration in the dopamine solution promotes a homogeneous and smooth coating with a drastic increase of the deposition rate. Also, the addition of oxygen into the dip coating process increases the corrosion resistance of the material.

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“…Therefore, biodegradable polymer materials have received much attention in recent years [1][2]. With the rapid development of commercialization of biodegradable plastic products, biodegradable materials have been widely used in packaging [3][4], agriculture [5][6], medical [7][8], 3D printing [9][10][11] and other fields.…”

Section: Introductionmentioning

confidence: 99%

Preparation of in-situ compatibilized PLA/starch composites and its non-isothermal pyrolysis kinetics

Sun

et al. 2020

E3S Web Conf.

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A fully degradable PLA/starch composite was prepared by one-step in-situ compatibilization method. The effects of raw materials ratio and maleic anhydride （MAH） dosage were studied by thermogravimetric analyzer (TG) and scanning electron microscopy (SEM). The thermal stability and microstructure of the composites were investigated, and the non-isothermal thermal decomposition kinetics of the composites were discussed. The DTG results show that there are two distinct maximum decomposition rate peaks in the unmodified composites. After the MAH is added, the maximum decomposition rate peaks of the composites are close to each other. In addition, as the MAH dosage increases, the maximum rate of decomposition peak gradually shifts to a lower temperature. The SEM results show that the compatibility of the composites is improved after the addition of MAH. And both the Kissinger and Flynn-Wall-Ozawa methods are well described for the non-isothermal thermal decomposition kinetics of PLA and PLA/starch composites.

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In vitro and in vivo investigation of PLA/PCL scaffold coated with metformin-loaded gelatin nanocarriers in regeneration of critical-sized bone defects

Cited by 101 publications

References 46 publications

Effect of metformin on human periodontal ligament stem cells cultured with polydopamine‐templated hydroxyapatite

Effect of metformin on human periodontal ligament stem cells cultured with polydopamine‐templated hydroxyapatite

Investigation of the Oxidation Mechanism of Dopamine Functionalization in an AZ31 Magnesium Alloy for Biomedical Applications

Preparation of in-situ compatibilized PLA/starch composites and its non-isothermal pyrolysis kinetics

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