In vitro and in vivo evaluation of gelatin–chondroitin sulphate hydrogels for controlled release of antibacterial proteins

Kuijpers, A.J.

doi:10.1016/s0142-9612(00)00064-8

Cited by 92 publications

(43 citation statements)

References 14 publications

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“…In particular, their use in pharmaceutics shows promise since recent advancements in biotechnology have led to a great variety of pharmacologically active peptides and proteins that are not adequately released from systems that are not biodegradable. Degradation of the hydrogel microspheres not only allows a tailored release of entrapped molecules, but also circumvents the removal of the empty device from the body [30][31][32] . Although the use of biodegradable hydrogel microspheres related to prolonged and/or controlled drug delivery has been widely investigated, the need for the development of hydrogels using simpler and more feasible methods still exists.…”

Section: Discussionmentioning

confidence: 99%

Preparation of recombinant human bone morphogenetic protein-2 loaded dextran-based microspheres and their characteristics1

Chen

Wang

et al. 2005

Acta Pharmacologica Sinica

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Aim: To prepare new pharmaceutical forms with sustained delivery properties of recombinant human bone morphogenetic protein-2 (rhBMP 2 ) for tissue engineering and guided tissue regeneration (GTR) use. Methods: rhBMP 2 -loaded dextranbased hydrogel microspheres (rhBMP 2 -MPs), which aimed to keep rhBMP 2 bioactivity and to achieve long-term sustained release of rhBMP 2 , were prepared by double-phase emulsified condensation polymerization. The physical, chemical performances and biological characteristics of those microspheres were studied both in vitro and in vivo. Results: The microspheres' average diameter was 30.33±4.32 µm with 75.4% ranging from 20 µm to 40 µm and the drug loading and encapsulation efficiency were 7.82% and 82.25%, respectively. The rhBMP 2 -releasing profiles in vitro showed that rhBMP 2 release could be maintained more than 10 d. The rhBMP 2 -MPs, with good swelling and biodegradation behavior, could be kept for 6 months at below 4 ºC without significant characteristic change or bioactivity loss. Cytology studies showed that rhBMP 2 -MPs could promote the proliferation of periodontal ligament cells (PDLCs) approximately 10 d, while the bioactivity of concentrated rhBMP 2 solution could keep no more than 3 d. Scanning electron microscope showed that rhBMP 2 -MPs could be enchased into the porous structure of calcium phosphate ceremic (CPC) and the eugonic growth of PDLCs in CPC/rhBMP 2 -MPs scaffolds. Animal experiments indicated that using CPC/rhBMP 2 -MPs scaffolds could gain more periodontal tissue regeneration than using rhBMP 2 compound firsthand with CPC (CPC/rhBMP 2 ). Conclusion: By encapsulating rhBMP 2 into dextran-based microspheres, a small quantity of rhBMP 2 could achieve equivalent effects to the concentrated rhBMP 2 solution and at the same time, could prolong rhBMP 2 retention both in vitro and in vivo.

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Section: Discussionmentioning

confidence: 99%

Preparation of recombinant human bone morphogenetic protein-2 loaded dextran-based microspheres and their characteristics1

Chen

Wang

et al. 2005

Acta Pharmacologica Sinica

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“…The Bio-gel p-60 column was conditioned by sodium phosphate buffer (0.01 M) and NaCl solution (0.15 M, contain 2% BSA) for isolating free iodine from 125 I labeled anti-HSA. In order to immobilize 125 I anti-HSA monoclonal antibody, the 11-mercaptoundecanoic acid/Au surface was immersed in the solution containing coupling agents: 75 mM N-ethyl-N'-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC, E-6383, Sigma) and 15 mM N-hydroxysuccinimide (NHS, H-7377, Sigma) at 4 °C for 30 min (van Delden et al, 1997;Kuijpers et al, 2000). Water-soluble EDC and NHS were used to activate O=C-OH (Kang et al, 1993;Tyan et al, 2002) and then the EDC-NHS solution was replaced by a phosphate buffered saline (PBS, URPBS001, UniRegion Bio-Tech), containing 0.2 μg/mL HSA-antibody at 4 °C for 24 hrs.…”

Section: Immobilization Of Anti-hsamentioning

confidence: 99%

Quartz crystal microbalance in clinical application

Yang¹,

Jong²,

Chung³

et al. 2011

Biosensors for Health, Environment and Biosecurity

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“…The absence of monomers was ensured spectrometrically by ensuring that the water of the washings did not contain any trace of monomers. The SPH was dried in an oven at 60°C for 6 hours [5][6][7][8].…”

Section: Synthesis Of Superporous Hydrogel Compositementioning

confidence: 99%

Synthesis of fast swelling superporous hydrogel: effect of concentration of crosslinker and acdisol on swelling ratio and mechanical strength.

Kumar¹,

Pandey²,

Koshy³

et al. 2010

Int j Drug delivery

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Fast swelling highly porous superporous hydrogels (SPH) are synthesized through a method utilizing rapid solution polymerization of acrylic acid for development of gastric retention devices. The swelling property, mechanical strength and release profile of SPH containing Metformin was investigated by changing the amount of crosslinking agents, (Bis) and AcDiSol. The results indicate that swelling ratio increases in the concentration range of 1%-2.5% w/v of Bis while beyond 2.5% w/v the swelling ratio slightly decreases but is not significant. Mechanical strength of the SPH was significantly increased by increasing the concentration of Bis from 1%-3.5% w/v. The release kinetics of SPH containing different crosslinker concentrations was measured in 0.1NHCl which was found to be consistent with the expected swelling behavior. AcDiSol was an important factor in maintaining the capillary structure required for fast swelling. Increase in AcDiSol resulted in decrease in swelling ratio of composite from 98.36±0.5% to 85.57±0.4%. As the amount of AcDiSol increases the mechanical strength increases due to increased cross linking density of the Superporous Hydrogel. This study indicates that superporous hydrogel composite possessed two properties necessary for gastric retention i.e., fast swelling and mechanical strength.

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In vitro and in vivo evaluation of gelatin–chondroitin sulphate hydrogels for controlled release of antibacterial proteins

Cited by 92 publications

References 14 publications

Preparation of recombinant human bone morphogenetic protein-2 loaded dextran-based microspheres and their characteristics1

Preparation of recombinant human bone morphogenetic protein-2 loaded dextran-based microspheres and their characteristics1

Quartz crystal microbalance in clinical application

Synthesis of fast swelling superporous hydrogel: effect of concentration of crosslinker and acdisol on swelling ratio and mechanical strength.

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