In Vitro and In Vivo Protective Effects of Lentil (Lens culinaris) Extract against Oxidative Stress-Induced Hepatotoxicity

Abstract

Excessive oxidative stress plays a role in hepatotoxicity and the pathogenesis of hepatic diseases. In our previous study, the phenolic extract of beluga lentil (BLE) showed the most potent in vitro antioxidant activity among extracts of four common varieties of lentils; thus, we hypothesized that BLE might protect liver cells against oxidative stress-induced cytotoxicity. BLE was evaluated for its protective effects against oxidative stress-induced hepatotoxicity in AML12 mouse hepatocytes and BALB/c mice. H2O2 treatment caused a marked decrease in cell viability; however, pretreatment with BLE (25–100 μg/mL) for 24 h significantly preserved the viability of H2O2-treated cells up to about 50% at 100 μg/mL. As expected, BLE dramatically reduced intracellular reactive oxygen species (ROS) levels in a dose-dependent manner in H2O2-treated cells. Further mechanistic studies demonstrated that BLE reduced cellular ROS levels, partly by increasing expression of antioxidant genes. Furthermore, pretreatment with BLE (400 mg/kg) for 2 weeks significantly reduced serum levels of alanine transaminase and triglyceride by about 49% and 40%, respectively, and increased the expression and activity of glutathione peroxidase in CCl4-treated BALB/c mice. These results suggest that BLE protects liver cells against oxidative stress, partly by inducing cellular antioxidant system; thus, it represents a potential source of nutraceuticals with hepatoprotective effects.

Antioxidative potential and ameliorative effects of green lentil (Lens culinaris M.) sprouts against CCl4-induced oxidative stress in rats

“…Our recent in vivo study indicated that LSHE stated substantial reductions in FBG in rats in a dose-dependent manner, as similarly indicated (13,23,51). These results strengthen our analysis, which confirms that LSHE possesses hypoglycemic effects because of rich polyphenols as effective antioxidants capable of modulating glucose levels (9,15). Practically, the administration of LSHE was extremely beneficial in body weight recovery in a dosedependent manner (57,61).…”

“…Our results demonstrated the efficacy of LSHE as a rich source of antioxidants that alleviated liver malfunctions and elevated serum lipids among CCl 4 -intoxicated rat groups. It might be due to increasing and supporting rats' serum with bioactive dietary antioxidants (9,15,62). Administration of LSHE at 50 or 100 mg GAE Kg −1 improved the lipid profile in dose-dependent manure.…”

“…It has been claimed that lentil extracts have a variety of pharmacological effects both in vivo and in vitro, including antidiabetic, hypotensive, hypolipidemic, and cardioprotective effects (7). A study has also shown that the phenolic extract from lentils possesses direct ROS scavenging abilities (14,15). According to Jung et al (15), the phenolic extract of L. culinaris can serve as a possible source of nutraceuticals with hepatoprotective properties since it partially protects liver cells from oxidative stress by triggering the cellular antioxidant system.…”

“…A study has also shown that the phenolic extract from lentils possesses direct ROS scavenging abilities (14,15). According to Jung et al (15), the phenolic extract of L. culinaris can serve as a possible source of nutraceuticals with hepatoprotective properties since it partially protects liver cells from oxidative stress by triggering the cellular antioxidant system. According to Goudarzi et al (12), sodium arsenite (SA) can significantly increase oxidative stress while also depleting antioxidant reserves and blocking the actions of antioxidant enzymes.…”

Detoxification technology and mechanism of processing with Angelicae sinensis radix in reducing the hepatotoxicity induced by rhizoma Dioscoreae bulbiferae in vivo

“…Numerous studies have shown that oxidative stress occupied prominent values in the pathogenesis of hepatotoxicity ( Guo et al, 2019 ; Lv et al, 2020 ; Temel et al, 2020 ; Jung et al, 2021 ; Lim et al, 2021 ). When the body’s oxidation and anti-oxidation are out of balance, excess reactive oxygen species will be produced, resulting in a state of oxidative stress, lipid peroxidation of cell membranes, and abnormal organelle function ( Bao et al, 2010 ; Glade and Meguid, 2017 ; Hussein et al, 2020 ).…”