In vitro and In vivo Antioxidant evaluation and estimation of total phenolic, flavonoidal content of Mimosa pudica L

Patro, Ganesh; Bhattamisra, Subrat Kumar; Mohanty, Bijay Kumar; Sahoo, Himanshu Bhusan

doi:10.4103/0974-8490.171099

Cited by 35 publications

(26 citation statements)

References 29 publications

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“…EAEAC has the highest scavenging activity followed by HEAC and AQEAC. These results in agreement with many previous studies who found that the level of TPC and TFC in extract play a major role for antioxidant capacity [ 36 – 39 ]. The difference of antioxidant capacities among HEAC, EAEAC and AQEAC was due to the solvent used during partitioning.…”

Section: Discussionsupporting

confidence: 93%

In vitro investigation of cytotoxic and antioxidative activities of Ardisia crispa against breast cancer cell lines, MCF-7 and MDA-MB-231

Nordin

Kadir

Zakaria

et al. 2018

BMC Complement Altern Med

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BackgroundArdisia crispa Thunb. D.C is used mostly in some parts of the Asian region by traditional practitioners to treat certain diseases associated with oxidative stress and inflammation including cancer and rheumatism. In Malaysia, it is popularly known as ‘Mata Ayam’ and local traditional practitioners believed that the root of the plant is therapeutically beneficial.MethodsThe cytotoxic effect of hydromethanolic extract of A. crispa and its solvents partitions (ethyl acetate and aqueous extracts) against breast cancer cells were evaluated by using MTT assay. The cells were treated with concentration of extracts ranging from 15.63 μg/mL- 1000 μg/mL for 72 h. The quantification of phenolic and flavonoid contents of the extracts were carried out to determine the relationship between of phytochemical compounds responsible for cytotoxic and antioxidative activities. The antioxidant capacity was measured by DPPH and ABTS free radical scavenging assay and expressed as milligram (mg) Trolox equivalent antioxidant capacity per 1 g (g) of tested extract.ResultsThe hydromethanolic and ethyl acetate extracts showed moderate cytotoxic effect against MCF-7 with IC50 values of 57.35 ± 19.33 μg/mL, and 54.98 ± 14.10 μg/mL, respectively but aqueous extract was inactive against MCF-7. For MDA-MB-231, hydromethanolic, ethyl acetate and aqueous extracts exhibited weak cytotoxic effects against MDA-MB-231 with IC50 values more than 100 μg/mL. The plant revealed high total phenolic content, total flavonoid and antioxidant capacity.ConclusionThe response of different type of breast cancer cell lines towards A. crispa extract and its partitions varied. Accordingly, hydromethanolic and ethyl acetate extracts appear to be more cytotoxic to oestrogen receptor (ER) positive breast cancer than oestrogen receptor (ER) negative breast cancer. However, aqueous extract appears to have poor activity to both types of breast cancer. Besides that, hydromethanolic and ethyl acetate extracts exhibit higher TPC, TFC and antioxidant capacity compared to aqueous extract. Synergistic effect of anticancer and antioxidant bioactives compounds of A. crispa plausibly contributed to the cytotoxic effects of the extract.

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Section: Discussionsupporting

confidence: 93%

In vitro investigation of cytotoxic and antioxidative activities of Ardisia crispa against breast cancer cell lines, MCF-7 and MDA-MB-231

Nordin

Kadir

Zakaria

et al. 2018

BMC Complement Altern Med

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“…Its roots and leaves are used in hemorrhoids and fistula treatments and against convulsions (Hassan et al, 2010). In vitro and in vivo analysis of flavonoids and phenolic compounds of leaves, stems, and seeds of the M. pudica have found high antioxidant, antimicrobial, anticancer and antidiabetic activities, justifying its use in different diseases treatments (Doss et al, 2011;Kaur et al, 2011;Zhang et al, 2011;Joseph et al, 2013;Gunawardhana et al, 2015;Tunna et al, 2015;Jose et al, 2016;Muhammad et al, 2016;Patro et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

An improved protocol for in vitro propagation of the medicinal plant Mimosa pudica L.

Bianchetti¹,

Resende²,

Pacheco³

et al. 2017

Afr. J. Biotechnol.

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This work aimed to develop a protocol for the in vitro establishment, multiplication, rooting and ex vitro acclimatization of Mimosa pudica L., a species used in folk medicine and with pharmacological activity. Aseptic cultures were established from seeds inoculated in MS medium, without growth regulators, followed by an in vitro stabilization phase in culture medium supplemented with 2.22 μM BAP. The cultures were transferred to MS medium supplemented with different cytokinins, combined or not with an auxin, aiming its large-scale propagation. The culture medium supplemented with 5 µM BAP plus 0.5 µM NAA provided the highest multiplication rate and top quality plantlets. The combination of 0.6 µM TDZ plus 0.05 µM NAA resulted in higher multiplication rates than in response to combination of BAP plus NAA, although the subsequent maintenance of the cultures in a medium without growth regulators has resulted in low regenerative response. In vitro rooting of micro-cuttings was high even in the absence of auxins. Over 90% of plantlets transferred to the greenhouse survived after the acclimatization phase. Acclimatized plants presented normal vegetative and reproductive development. The procedures established in the present study allow a massive production of M. pudica plants for further pharmacological studies.

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“…As shown in Figure 4A, compare to the preselected positive components, okicamelliaside has a specific free radical scavenging rate at a dose of 10 µM. Next, the activities of SOD and CAT on 293T cells, which are two important antioxidant indices, were also evaluated (Patro et al., 2016). The results are shown in Figure 4B and C; compared with the Con group, okicamelliaside, gallic acid, epicatechin, and salicylic acid group all caused a significant decrease in CAT and SOD activity in a concentration‐dependent manner.…”

Section: Resultsmentioning

confidence: 99%

Integrated molecular network and HPLC‐UV‐FLD analysis to explore antioxidant ingredients in camellia nitidissima Chi

Cheng

Hou

Hao

et al. 2021

Journal of Food Science

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At present, screening of active ingredients from natural products for pharmacological and clinical research is mostly time-consuming and costly. In this study, a molecular network (MN) guided high performance liquid chromatography-ultraviolet-fluorescence detector (HPLC-UV-FLD) method was carried out to profile the global antioxidant activity compounds, including the trace amount ingredients in Camellia nitidissima Chi (CNC). Firstly, HPLC-UV-FLD postcolumn derivatization system was utilized to screen the antioxidants. Then the MN of CNC was established via mass spectrometry (MS) data for getting the connection between ingredient structures. As a result, HPLC-UV-FLD indicated three antioxidant ingredients: gallic acid (126.3 mg/g), catechin (564.8 mg/g), and salicylic acid (24.3 mg/g). Combined with the MN, the actives' precise location and connection relationship were clarified based on the structural similarities. A new antioxidant ingredient, okicamelliaside, was suggested and evaluated at free radical scavenging and enzymatic protection. The novel method of activity and structural correlation analysis based on MN could provide a useful guide for screening trace active ingredients in natural products.Practical Application: Three main ingredients were screened out from Camellia nitidissima Chi by HPLC-UV-FLD postcolumn derivatization system. Integrated molecular network and HPLC-UV-FLD analysis, a new type of antioxidant okicamelliaside was selected. The novel method of activity and structural correlation analysis based on molecular network could provide a useful guide for screening trace active ingredients in natural products.

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In vitro and In vivo Antioxidant evaluation and estimation of total phenolic, flavonoidal content of Mimosa pudica L

Cited by 35 publications

References 29 publications

In vitro investigation of cytotoxic and antioxidative activities of Ardisia crispa against breast cancer cell lines, MCF-7 and MDA-MB-231

In vitro investigation of cytotoxic and antioxidative activities of Ardisia crispa against breast cancer cell lines, MCF-7 and MDA-MB-231

An improved protocol for in vitro propagation of the medicinal plant Mimosa pudica L.

Integrated molecular network and HPLC‐UV‐FLD analysis to explore antioxidant ingredients in camellia nitidissima Chi

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