In Vitro and In Vivo Performance of Plum (Prunus domestica L.) Pollen from the Anthers Stored at Distinct Temperatures for Different Periods

Đorđević, M.; Vujović, T.; Cerović, R.; Glišić, Ivana; Milošević, Nebojša; Maric, S.; Radičević, S.; Akšić, Milica Fotirić; Meland, Mekjell

doi:10.3390/horticulturae8070616

Cited by 8 publications

(6 citation statements)

References 44 publications

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“…The three water contents of pollens set in this study played a certain role in the pollen preservation, but when the temperature was reduced to −20 • C or below, pollen viability was no longer largely determined by the pollen water content. It may be caused by an insufficient gap between water content gradients [71,75]. A suitable method for short-term pollen preservation of G. sinensis was identified in this study.…”

Section: Pollen Preservation Of G Sinensismentioning

confidence: 92%

“…It was found that the pollen germination rate was easily affected by storage temperature, storage time and pollen water content during pollen storage of G. sinensis in this study. A common feature of all pollen preservation treatments was a decrease in germination rate with increasing storage time, such as plum [75], pecan [49], rose [15], litchi [76] and chrysanthemum [77]. It was almost impossible for pollen stored at RT to germinate after 30 days, but it was still possible to maintain pollen vitality at low temperatures.…”

Section: Pollen Preservation Of G Sinensismentioning

confidence: 99%

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Studies on Pollen Morphology, Pollen Vitality and Preservation Methods of Gleditsia sinensis Lam. (Fabaceae)

Wang

et al. 2023

Forests

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Gleditsia sinensis Lam. (Fabaceae) is an endemic species in China, which has a wide range of ecological functions and high economic value. G. sinensis belongs to androdioecy, and the stamens of perfect flowers are aborted, meaning that a perfect flower is a functional female flower. Understanding the dynamic process of flowering and the characteristics of pollen morphology effectively determine the viability of pollen vitality, and the suitable conditions for short-term storage of pollen can provide theoretical basis and technical reference for hybrid breeding and germplasm conservation of G. sinensis. In this study, the male plants of G. sinensis in Guiyang area were used as research materials. The flowering dynamic process of male flowers was recorded through field observation. The morphology of pollen was observed and analyzed with a scanning electron microscope (SEM). The germination characteristics of pollen were studied with an in vitro germination method, and the pollen vitality was also determined using four staining methods. The effects of different storage temperatures and water contents on pollen germination rate were discussed. The results showed that the male flowers of G. sinensis had a short, single flowering period, lasting 2–3 days from the opening to the shedding. The dynamic opening process of a single flower was artificially divided into five stages. Pollen grains of G. sinensis are oblate spheroidal, tricolporate with equatorial elongated endoapertures and the sporoderm surface is reticulate. The MTT (Thiazolyl Blue Tetrazolium Bromide) staining method could accurately and quickly determine the pollen vitality of G. sinensis. The highest pollen germination rate was 65.89% ± 3.41%, and the length of the pollen tube was 3.96 mm after cultured in 15% sucrose + 100 mg/L boric acid + 20 mg/L calcium chloride for 24 h. It was necessary to collect the pollen at the big bud stage, which was conducive to improving the efficiency of pollen collection because the pollen had been mature with high pollen vitality at this stage. When it came to pollen preservation, the pollen germination rate was significantly affected by storage time, storage temperature and pollen water content. The pollen still had high vitality after being stored at −80 °C for 30 days when the moisture content of the pollen decreased to 9%, and the pollen germination rate only decreased by 28.84% compared with that before storage. In conclusion, this study has comprehensively and systematically studied the morphology, vitality determination and preservation methods of the pollen of G. sinensis, providing a theoretical basis for the cross regional breeding and the conservation and utilization of germplasm resources.

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Section: Pollen Preservation Of G Sinensismentioning

confidence: 92%

Section: Pollen Preservation Of G Sinensismentioning

confidence: 99%

Studies on Pollen Morphology, Pollen Vitality and Preservation Methods of Gleditsia sinensis Lam. (Fabaceae)

Wang

et al. 2023

Forests

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“…However, there has been no published report about how many days caprifig fruits can be stored at 4 • C or while using an alternative storage temperature without losing pollen viability and germination. Several studies have shown that storing pollen at low temperatures is effective for their long-term preservation, including almond [17], cherimoya [18], mango [19], cherry [20], apple [21], pecan [22], gấc melon [23], plum [24], and rose [25]. However, because the fig's pollen ripens in the male fig fruits (caprifig), no studies have been found to determine the effect of pollen storage within caprifigs on the viability and germination of the pollen.…”

Section: Introductionmentioning

confidence: 99%

Effects of Caprifig (Ficus carica var. caprificus) Storage Temperature and Duration on the Fruit Productivity and Quality of ‘Bursa Siyahi’ Figs

KOŞAR

Ertürk

2023

Horticulturae

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Caprification is the process of hanging caprifig fruits on edible fig trees to transfer the pollen inside the caprifig to the edible fig via the wasp (Blastophaga psenes) living in the caprifig. It needs to be repeated several times for the proper fruit set of edible figs. The present study was conducted to determine the change in the number of Blastophaga psenes, the duration of Blastophaga’s exit, and pollen viability in case the caprifigs to be used in the caprification process are stored until use. The number of Blastophaga and in vitro pollen viability were tested at day 0 (harvest day) and after 4, 8, 12, 16 and 20 days of storage at three different temperatures (0, 4, and 8 °C). Afterwards, the effect of pollination frequency on the edible fig fruit set and quality was determined by using Blastophaga psenes as a vector in the pollination of stored caprifig fruits, pollinating five times with 4-day intervals and three times with 8-day intervals. Approximately a 50% reduction in the number of B. psenes was detected after 4 (180.22), 12 (174.11) and 16 (192.66) days of caprifigs storage at 0, 4, and 8 °C, respectively. The pollen germination percentage of the caprifigs increased with storage and was higher in those stored at 8 °C (43.96%) and 4 °C (41.70%). The highest fruit set was obtained when the caprifigs stored at 4 °C (76.41%) and 8 °C (71.38%) five times with 4-day intervals were used for pollination. The pollination practice repeated five times with 4-day intervals resulted in a lower proportion of extra-large fruits with a weight of >100 g, a higher proportion of fruits with no or slight ostiole damage and early ripening of fruits. These results suggest that B. psenes and pollen viability can be preserved by storing caprifigs at 4 or 8 °C and that fruit set and fruit characteristics would be positively affected with the use of stored caprifigs in the pollination practice repeated five times with 4-day intervals.

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“…In such programs, the need to store pollen without losing germinability and fertility arises when it comes to larger hybridizations, in the case of nonsynchronous flowering and/or geographical isolation of parental species, or when dealing with the low vitality of currently available fresh pollen. So far, there are several reports in different fruit species showing that pollen stored at low temperatures is effective for short-or long-term preservation period (Martínez-Gómez et al, 2002;Alburquerque et al, 2007;Beltrán et al, 2019;^ali} et al, 2021;\or|evi} et al, 2022). About 70% of flowering plants disperse pollen at the binucleate stage, consisting of a vegetative nucleus and a generative nucleus ‡ the latter being the precursor of the two sperm cells (Brewbaker, 1967).…”

Section: Introductionmentioning

confidence: 99%

In vitro Performance of Fresh and Stored Plum (Prunus domestica L.) Pollen

Đorđević,

Vujović,

Cerović

et al. 2023

Voćarstvo = Journal of Pomology

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In this study, the in vitro performance of fresh and pollen stored for three months at different constant temperatures was investigated in three plum cultivars ('Valerija', '^a~anska Lepotica', 'Valjevka'). Pollen was collected from flowers in the late balloon phase and, after drying, stored for three months at four different constant temperatures (+4°C, -20°C, -80°C, -196°C). Pollen tube length as well as the nuclear presence in the pollen tube and its migration were examined. In vitro pollen tube length was tested after a 24-hour germination period, after which the pollen tube was stained with Hoechst 33258 to observe the nuclear presence within it. The longest average pollen tube length in all tested cultivars was observed with fresh pollen. With the pollen stored at low temperatures, a longer pollen tube was obtained compared to pollen stored at +4°C. In all cultivars, mature pollen grains at the binucleate stage contained a vegetative nucleus (VN) and a generative nucleus (GN). Although at each storage temperature a higher percentage of GN was achieved in pollen tubes compared to fresh ones, nevertheless, regular movement of nuclei, primarily VN rather than GN or sperm nucleus (SN), was found in the highest percentage in all tested cultivars.

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In Vitro and In Vivo Performance of Plum (Prunus domestica L.) Pollen from the Anthers Stored at Distinct Temperatures for Different Periods

Cited by 8 publications

References 44 publications

Studies on Pollen Morphology, Pollen Vitality and Preservation Methods of Gleditsia sinensis Lam. (Fabaceae)

Studies on Pollen Morphology, Pollen Vitality and Preservation Methods of Gleditsia sinensis Lam. (Fabaceae)

Effects of Caprifig (Ficus carica var. caprificus) Storage Temperature and Duration on the Fruit Productivity and Quality of ‘Bursa Siyahi’ Figs

In vitro Performance of Fresh and Stored Plum (Prunus domestica L.) Pollen

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