In vitro and in vivo evaluation of biotin-mediated PEGylated nanostructured lipid as carrier of disulfiram coupled with copper ion

Liu, Xia; Chu, Hui Juan; Cui, Nan; Wang, Tianying; Dong, Shengyi; Cui, Siwen; Dai, Yinghui; Wang, Dongkai

doi:10.1016/j.jddst.2019.03.037

Cited by 11 publications

(7 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In Vitro Drug Release Analysis. The cumulative release of DSF from the Ch/DSF hydrogel in vitro was measured by the dialysis membrane method in PBS (pH 6.8 & 7.4) at 37 °C for 24 h. 44 Approximately, 40% of DSF was released in the first hour at pH 6.8, while 35% at pH 7.4. After that, the release was slightly enhanced, reaching 97 and 95% drug release at pH 6.8 and 7.4, respectively (Figure 6).…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Thermosensitive Chitosan-Based Injectable Hydrogel as an Efficient Anticancer Drug Carrier

2020

View full text Add to dashboard Cite

A thermosensitive, physically cross-linked injectable hydrogel was formulated for the effective and sustained delivery of disulfiram (DSF) to the cancer cells as there is no hydrogel formulation available until now for the delivery of DSF. As we know, hydrogels have an advantage over other drug delivery systems because of their unique properties, so we proposed to formulate an injectable hydrogel system for the sustained delivery of an anticancer drug (DSF) to cancer cells. To investigate the surface morphology, a scanning electron microscope study was carried out, and for thermal stability of hydrogels, TGA (thermogravimetric analysis) and DSC (differential scanning calorimetry) were performed. The rheological behavior of hydrogels was evaluated with the increasing temperature and time. These developed hydrogels possessing excellent biocompatibility could be injected at room temperature following rapid gel formation at body temperature. The swelling index and in vitro drug release studies were performed at different pH (6.8 and 7.4) and temperatures (25 and 37 °C). The cell viability of the blank hydrogel, free DSF solution, and Ch/DSF (chitosan/DSF)-loaded hydrogel was studied by MTT assay on SMMC-7721 cells for 24 and 48 h, which exhibited higher cytotoxicity in a dose-dependent manner in contrast to the free DSF solution. Moreover, the cellular uptake of DSF-loaded hydrogels was observed stronger as compared with free DSF. Hence, chitosan-based hydrogels loaded with DSF possessing exceptional properties can be used as a novel injectable anticancer drug for the sustained delivery of DSF for long-term cancer therapy.

show abstract

Section: Resultsmentioning

confidence: 99%

“…The cumulative release of DSF from the Ch/DSF hydrogel in vitro was measured by the dialysis membrane method in PBS (pH 6.8 & 7.4) at 37 °C for 24 h . Approximately, 40% of DSF was released in the first hour at pH 6.8, while 35% at pH 7.4.…”

Section: Resultsmentioning

confidence: 99%

Thermosensitive Chitosan-Based Injectable Hydrogel as an Efficient Anticancer Drug Carrier

2020

View full text Add to dashboard Cite

show abstract

“…The in vitro DSF release of DSF powder and DSF-SPI-Ns were performed with a dialysis membrane (MWCO = 3500 kDa). 29 The phosphate buffer saline (PBS) pH 5.5 and 7.4 was used as a release medium. 2.5 mg of DSF powder or DSF-SPI-Ns were dispersed in 3 mL of distilled water and incubated in 100 mL of PBS pH 5.5 and 7.4 at 37 °C with constant stirring speed (100 rpm).…”

Section: Methodsmentioning

confidence: 99%

Globular protein stabilized nanoparticles for delivery of disulfiram: fabrication, characterization, in vitro toxicity, and cellular uptake

Farooq

Parveen

et al. 2020

RSC Adv.

View full text Add to dashboard Cite

Disulfiram (DSF), an FDA-approved anti-alcoholic drug, has recently shown that it possesses anti-cancer effects. However, DSF is hydrophobic in nature with less stability. Therefore, new approaches are required for the effective delivery of DSF to treat cancers. Herein, we prepared DSF loaded soy protein isolate (SPI) nanosuspension (Ns) for enhancing the anti-cancer delivery of DSF. The optimized DSF-SPI-Ns had an average particle size of 164.28 AE 2.07 nm with a narrow size distribution of 0.217 AE 0.035 and zeta potential around À22.30 AE 2.11 mV, respectively. The highest drug loading and entrapment efficiency achieved was 5.516 AE 1.98%, and 91.61 AE 1.15%, respectively. The surface morphology of Ns was revealed by TEM, and the FTIR DSC, PXRD, and TGA were used for physicochemical characterization. Further, fluorescence spectroscopy and molecular docking studies were carried out to understand the interactions between (SPI and DSF) and binding sites of DSF on the surface of SPI, respectively. In vitro release studies showed a sustained release pattern and followed a Fickian diffusion release from the Ns. The in vitro cytotoxicity of SPI indicated the excellent biocompatibility, and DSF-SPI-Ns were found to be more cytotoxic compared to the free DSF solution. Moreover, the cellular uptake studies also indicated the effective delivery of the formulation to the cancer cells. Results of the current study suggested that the SPI coated Ns might be a promising drug delivery system for hydrophobic DSF, and the potential application of SPI as a coating/stabilizing agent for the delivery of hydrophobic/hydrophilic cancer therapeutics.

show abstract

“…Apart from polymers, there are also lipid-based carriers with biotin modification. Recently, biotin-polyethylene glycol 2000-distearyl phosphatidyl ethanolamine (biotin-PEG-DSPE) was used in the emulsification-ultrasonic and low temperature-solidification method to obtain nanostructured lipid carriers (NLCs) for disulfiram delivery in the presence of copper ion [ 128 ]. DDSs exhibited good stability and cytotoxicity toward breast cancer cells (4T1) in vitro and in vivo.…”

Section: Biotin—targeted Nanoparticlesmentioning

confidence: 99%

Single- versus Dual-Targeted Nanoparticles with Folic Acid and Biotin for Anticancer Drug Delivery

et al. 2021

View full text Add to dashboard Cite

Cancer is one of the major causes of death worldwide and its treatment remains very challenging. The effectiveness of cancer therapy significantly depends upon tumour-specific delivery of the drug. Nanoparticle drug delivery systems have been developed to avoid the side effects of the conventional chemotherapy. However, according to the most recent recommendations, future nanomedicine should be focused mainly on active targeting of nanocarriers based on ligand-receptor recognition, which may show better efficacy than passive targeting in human cancer therapy. Nevertheless, the efficacy of single-ligand nanomedicines is still limited due to the complexity of the tumour microenvironment. Thus, the NPs are improved toward an additional functionality, e.g., pH-sensitivity (advanced single-targeted NPs). Moreover, dual-targeted nanoparticles which contain two different types of targeting agents on the same drug delivery system are developed. The advanced single-targeted NPs and dual-targeted nanocarriers present superior properties related to cell selectivity, cellular uptake and cytotoxicity toward cancer cells than conventional drug, non-targeted systems and single-targeted systems without additional functionality. Folic acid and biotin are used as targeting ligands for cancer chemotherapy, since they are available, inexpensive, nontoxic, nonimmunogenic and easy to modify. These ligands are used in both, single- and dual-targeted systems although the latter are still a novel approach. This review presents the recent achievements in the development of single- or dual-targeted nanoparticles for anticancer drug delivery.

show abstract

In vitro and in vivo evaluation of biotin-mediated PEGylated nanostructured lipid as carrier of disulfiram coupled with copper ion

Cited by 11 publications

References 31 publications

Thermosensitive Chitosan-Based Injectable Hydrogel as an Efficient Anticancer Drug Carrier

Thermosensitive Chitosan-Based Injectable Hydrogel as an Efficient Anticancer Drug Carrier

Globular protein stabilized nanoparticles for delivery of disulfiram: fabrication, characterization, in vitro toxicity, and cellular uptake

Single- versus Dual-Targeted Nanoparticles with Folic Acid and Biotin for Anticancer Drug Delivery

Contact Info

Product

Resources

About