In vitro and in vivo study of N-trimethyl chitosan nanoparticles for oral protein delivery

Fu, Chen; Zhang, Zhirong; Yuan, Fang; Qin, Xuan; Wang, Minting; Huang, Yuan

doi:10.1016/j.ijpharm.2007.07.035

Cited by 119 publications

(60 citation statements)

References 48 publications

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“…[184][185][186] Many early studies were conducted to deliver various drugs, including insulin, vaccines and proteins via sustained delivery with TMC-coated SLNs to enhance the biomedical effects in treating various chronic diseases. 187,[196][197][198] Fewer studies were conducted on the delivery of phytocompounds through TMC-coated SLNs.…”

Section: Tmc-coated Slnsmentioning

confidence: 99%

Recent developments in solid lipid nanoparticle and surface-modified solid lipid nanoparticle delivery systems for oral delivery of phyto-bioactive compounds in various chronic diseases

Ganesan

Ramalingam

Karthivashan

et al. 2018

IJN

135

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Solid lipid nanoparticle (SLN) delivery systems have a wide applicability in the delivery of phyto-bioactive compounds to treat various chronic diseases, including diabetes, cancer, obesity and neurodegenerative diseases. The multiple benefits of SLN delivery include improved stability, smaller particle size, leaching prevention and enhanced lymphatic uptake of the bioactive compounds through oral delivery. However, the burst release makes the SLN delivery systems inadequate for the oral delivery of various phyto-bioactive compounds that can treat such chronic diseases. Recently, the surface-modified SLN (SMSLN) was observed to overcome this limitation for oral delivery of phyto-bioactive compounds, and there is growing evidence of an enhanced uptake of curcumin delivered orally via SMSLNs in the brain. This review focuses on different SLN and SMSLN systems that are useful for oral delivery of phyto-bioactive compounds to treat various chronic diseases.

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Section: Tmc-coated Slnsmentioning

confidence: 99%

Recent developments in solid lipid nanoparticle and surface-modified solid lipid nanoparticle delivery systems for oral delivery of phyto-bioactive compounds in various chronic diseases

Ganesan

Ramalingam

Karthivashan

et al. 2018

IJN

135

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“…The cultures were kept in an atmosphere of 95% air and 5% CO 2 at 37 o C, and were used for the permeability experiments 18～21 days after seeding, when displayed TEER values of 400～550 Ω cm 2 . TEER values of the Caco-2 cell monolayers were monitored every 2～3 days with a Millicell-Electrical Resistance System (Millipore Corp., Bedford, MA, USA) connected to a pair of chopstick electrodes (13,15,16) In vitro Caco-2 permeability assay For in vitro Caco-2 permeability assay, the method of Markowska et al (17) was used, which is widely used for measuring cell permeable properties of particles. To initiate the transport experiment, the culture media in the apical and the basolateral compartments were aspirated, and the cells were rinsed twice with pre-warmed Hank's balanced salt solution (HBSS) at pH 7.4.…”

Section: Transepithelial Electrical Resistance (Teer) Measurements Dumentioning

confidence: 99%

Effect of Particle Size of Zinc Oxides on Cytotoxicity and Cell Permeability in Caco-2 Cells

Chang¹,

Choi²,

Ko³

et al. 2011

JFN

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The cell permeability and cytotoxic effects of different-sized zinc oxide (ZnO) particles were investigated using a human colorectal adenocarcinoma cell line called Caco-2. Morphological observation by scanning electron microscopy revealed that three zinc oxides with different mean particle sizes (ZnO-1, 20 nm; ZnO-2, 90～200 nm; ZnO-3, 1～5 μm) tended to aggregate, particularly in the case of ZnO-1. When cytotoxicities of all three sizes of zinc oxide particles were measured at concentration ranges of 1～1000 μg/mL, significant decreases in cell viability were observed at concentrations of 50 μg/mL and higher. Among the three zinc oxides, ZnO-1 showed the lowest viability at 50 μg/mL in Caco-2 cells, followed by ZnO-2 and ZnO-3. The permeate concentration of ZnO-1 from the apical to the basolateral side in the Caco-2 model system after four hours was about three-fold higher than that of either ZnO-2 or ZnO-3. These results demonstrated that ZnO-1, with a 20 nm mean particle size, had poorer viability and better permeability in Caco-2 cells than ZnO-2 and ZnO-3.

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“…[26][27][28] Previous studies have shown that chitosan (CS) and TMC NPs are efficient vaccine delivery systems for systemic vaccination. [29][30][31][32] In another study, Slutter et al compared CS and TMC NPs' function in oral immunization.…”

Section: Discussionmentioning

confidence: 99%

Oral immunization of mice with Omp31-loaded <em>N</em>-trimethyl chitosan nanoparticles induces high protection against <em>Brucella melitensis</em> infection

Abkar¹,

Fasihi-Ramandi²,

Kooshki³

et al. 2017

IJN

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Brucellosis is a group of closely associated zoonotic bacterial illnesses caused by members of the genus Brucella. B. melitensis Omp31 is a promising candidate for a subunit vaccine against brucellosis. This study surveyed the immunogenicity of Omp31 alone and with incomplete Freund’s adjuvant (Omp31-IFA) and N -trimethyl chitosan (TMC/Omp31) nanoparticles (NPs), as well as the effect of Omp31 immunization route on immunological responses and protection. After expression and purification, the recombinant Omp31 (rOmp31) was loaded onto TMC NPs by ionic gelation. The particle size, loading efficiency and in vitro release of the NPs were examined. Omp31-IFA was administered intraperitoneally, while TMC/Omp31 NPs were administered orally and intraperitoneally. According to the antibody subclasses and cytokine profile, intraperitoneal immunization by Omp31-IFA and TMC/Omp31 NPs induced T helper 1 (Th1) and Th1–Th2 immune responses, respectively. On the other hand, oral immunization with TMC/Omp31 NPs elicited a mixed Th1–Th17 immune response. Data obtained from the cell proliferation assay showed that vaccination with Omp31 stimulated a vigorous antigen-specific cell proliferative response, which could be further increased after oral immunization with TMC/Omp31 NPs. Vaccinated groups of mice when challenged with B. melitensis 16M were found to be significantly protected in the orally administered group in comparison with the intraperitoneally immunized mice. Results of this study indicated that the reason for high protection after oral vaccination can be via elicited Th17 response.

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In vitro and in vivo study of N-trimethyl chitosan nanoparticles for oral protein delivery

Cited by 119 publications

References 48 publications

Recent developments in solid lipid nanoparticle and surface-modified solid lipid nanoparticle delivery systems for oral delivery of phyto-bioactive compounds in various chronic diseases

Recent developments in solid lipid nanoparticle and surface-modified solid lipid nanoparticle delivery systems for oral delivery of phyto-bioactive compounds in various chronic diseases

Effect of Particle Size of Zinc Oxides on Cytotoxicity and Cell Permeability in Caco-2 Cells

Oral immunization of mice with Omp31-loaded <em>N</em>-trimethyl chitosan nanoparticles induces high protection against <em>Brucella melitensis</em> infection

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