In vitro and in vivo antioxidant properties of chlorogenic acid and caffeic acid

Abstract

Dietary polyphenols are thought to be beneficial for human health as antioxidants. Coffee beans contain a common polyphenol, chlorogenic acid. Chlorogenic acid is the ester of caffeic acid and quinic acid. Although these polyphenols have received much attention, there is little evidence indicating a relationship between the effect and the rate of absorption. In this study, we focused on the beneficial effects of chlorogenic acid and caffeic acid, a major metabolite of chlorogenic acid. We carried out in vitro and in vivo experiments. In the in vitro study, caffeic acid had stronger antioxidant activity than that of chlorogenic acid. The uptake of chlorogenic acid by Caco-2 cells was much less than that of caffeic acid. The physiological importance of an orally administered compound depends on its availability for intestinal absorption and subsequent interaction with target tissues. We then used an intestinal ischemia-reperfusion model to evaluate antioxidant activities in vivo. We found that both chlorogenic acid and caffeic acid had effects on intestinal ischemia-reperfusion injury. Since caffeic acid has a stronger antioxidant activity than that of chlorogenic acid and chlorogenic acid is hydrolyzed into caffeic acid in the intestine, it is possible that caffeic acid plays a major role in the protective effect of chlorogenic acid against ischemia-reperfusion injury.

High production of bioactive depsides in shoot and callus cultures of Aronia arbutifolia and Aronia × prunifolia

“…The first two DE mentioned have very valuable biological properties, of which the most important are: antioxidant, antiaggregatory, anti-inflammatory, immunostimulating, and anticancer activities (Nakatani et al 2000;Sato et al 2011;Meng et al 2013). Neochlorogenic acid (an isomer of chlorogenic acid) also shows similar properties (Dewick 1997;Thurow 2012).…”

Green and roasted coffee extracts as antioxidants in βTC3 cells with induced oxidative stress and lipid accumulation inhibitors in 3T3L1 cells, and their bioactivity in rats fed high fat diet

“…CHAs exhibit a broad range of biological activities: antibacterial, antifungal, hepatoprotective, antithrombotic, antiinflammatory and antioxidant activities [3][4][5][6][7][8]. Antioxidant activity of CHAs decrease the risk of several oxidative stress-related diseases, including atherosclerosis, some kinds of cancer and Alzheimer's disease [9][10][11].…”

Protective Effects of Chlorogenic Acid against Experimental Reflux Esophagitis in Rats

“…Biomol Ther 22(5), 420-425 (2014) www.biomolther.org rial and anti-carcinogenic activities (Belkaid et al, 2006;Sato et al, 2011). Moreover, we previously reported the protective effects of CGA against experimental inflammatory disorders: CGA inhibited high mobility group box 1 release and toll-like receptor 4 signaling pathways in liver during both septic and ischemic insult, suggesting its potential in immunomodulation Yun et al, 2012).…”