In vitro and in vivo studies of a novel nanohydroxyapatite/superhydrophilic vertically aligned carbon nanotube nanocomposites

Lobo, Anderson Oliveira; Siqueira, Idália A. W. B.; Neves, Marcele Florêncio das; Marciano, Fernanda Roberta; Corat, Evaldo José; Corat, Marcus Alexandre Finzi

doi:10.1007/s10856-013-4929-y

Cited by 18 publications

(19 citation statements)

References 25 publications

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“…4c). We have already observed this trend in a previous work, where [56] PDLLA/nHAp scaffolds promoted better in vitro osteogenic results and in vivo bone regeneration. However, herein we performed a deeper study involving bone formation using micro-CT and mechanical properties after scaffold implantation.…”

Section: Resultssupporting

confidence: 78%

Electrospun ultrathin PBAT/nHAp fibers influenced the in vitro and in vivo osteogenesis and improved the mechanical properties of neoformed bone

Santana-Melo

Rodrigues

Silva

et al. 2017

Colloids and Surfaces B: Biointerfaces

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Combining polyester scaffolds with synthetic nanohydroxyapatite (nHAp), which is bioactive and osteoconductive, is a plausible strategy to improve bone regeneration. Here, we propose the combination of PBAT [poly(butylene-adipate-co-terephthalate)] and synthetic nHAp (at 3 and 5wt%). PBAT is a relatively a new polymer with low crystallinity and attractive biodegradability and mechanical properties for orthopedic applications, however, with a still underexplored potential for in vivo applications. Then, we performed a careful biological in vitro and in vivo set of experiments to evaluate the influence of PBAT containing two different nHAp loads. For in vitro assays, osteoblast-like MG63 cells were used and the bioactivity and gene expression related to osteogenesis were evaluated by qRT-PCR. For in vivo experiments, twenty-four male rats were used and a tibial defect model was applied to insert the scaffolds. Micro-computed tomography (Micro-CT) and histological analysis were used to assess e bone neoformation after 6 weeks of implantation. Three point flexural tests measured the mechanical properties of the neoformed bone. All scaffolds showed promising in vitro properties, since they were not cytotoxic against MG-63 cells and promoted high cell proliferation and formation of mineralized nodules. From a mechanistic point-of-view, nHAp loading increased hydrophilicity, which in turn allowed for a better adsorption of proteins and consequent changes in the phenotypic expression of osteoblasts. nHAp induced better cellular responses on/in the scaffolds, which was mainly attributed to its osteoconductive and osteoinductive properties. Micro-CT images showed that nHAp at 3% and 5wt% led to more effective bone formation, presenting the highest bone volume after 6 weeks of implantation. Considering the three point flexural tests, 5wt% of nHAp positively influenced the flexural mode of the neoformed bone, but the stiffiness was similar between the 3% and 5wt% groups. In summary, this investigation demonstrated great potential for the application of these novel scaffolds towards bone regeneration and, thus, should be further studied.

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Section: Resultssupporting

confidence: 78%

Electrospun ultrathin PBAT/nHAp fibers influenced the in vitro and in vivo osteogenesis and improved the mechanical properties of neoformed bone

Santana-Melo

Rodrigues

Silva

et al. 2017

Colloids and Surfaces B: Biointerfaces

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“…25 CNTs, both single-walled and multiwalled types, are reported to have good osteoinductive and accelerating cell proliferative properties solely, or in combination with, nano-HA. 26 The fibroblast growth factor-incorporated CNTs have shown an increase in osteoblastic proliferation. 27 The porous chitosan scaffold, with varying degrees of nano-HA composition, has been studied by incorporating a single-walled magnetically produced CNT (B-SWCNT) and nonmagnetically produced H-SWCNT, where B-SWCNT was found to have superior biocompatibility properties.…”

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confidence: 99%

Regenerative nanomedicine: current perspectives and future directions

Chaudhury¹,

Kumar²,

Kandasamy³

et al. 2014

IJN

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Nanotechnology has considerably accelerated the growth of regenerative medicine in recent years. Application of nanotechnology in regenerative medicine has revolutionized the designing of grafts and scaffolds which has resulted in new grafts/scaffold systems having significantly enhanced cellular and tissue regenerative properties. Since the cell–cell and cell-matrix interaction in biological systems takes place at the nanoscale level, the application of nanotechnology gives an edge in modifying the cellular function and/or matrix function in a more desired way to mimic the native tissue/organ. In this review, we focus on the nanotechnology-based recent advances and trends in regenerative medicine and discussed under individual organ systems including bone, cartilage, nerve, skin, teeth, myocardium, liver and eye. Recent studies that are related to the design of various types of nanostructured scaffolds and incorporation of nanomaterials into the matrices are reported. We have also documented reports where these materials and matrices have been compared for their better biocompatibility and efficacy in supporting the damaged tissue. In addition to the recent developments, future directions and possible challenges in translating the findings from bench to bedside are outlined.

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“…Several papers have been published by our group reporting the use of superhydrophilic MWCNTO-GO as is or in bioscaffolds in contact with different cell lines [39][40][41][42]. In all of these studies, we have demonstrated their non-cytotoxicity and tissue regeneration ability using animal models [43]. Recently, we demonstrated that PDLLA membranes with low MWCNTO-GO content accelerated in vivo bone regeneration and induced bone lamellar formation [43].…”

Section: Biological Assaysmentioning

confidence: 91%

“…In all of these studies, we have demonstrated their non-cytotoxicity and tissue regeneration ability using animal models [43]. Recently, we demonstrated that PDLLA membranes with low MWCNTO-GO content accelerated in vivo bone regeneration and induced bone lamellar formation [43]. Herein, we advanced this issue since we conducted in vivo studies with positive biological responses using high amounts of MWCNTO-GO in composites.…”

Section: Biological Assaysmentioning

confidence: 99%

PDLLA honeycomb-like scaffolds with a high loading of superhydrophilic graphene/multi-walled carbon nanotubes promote osteoblast in vitro functions and guided in vivo bone regeneration

Silva

Vasconcellos

Rodrigues

et al. 2017

Materials Science and Engineering: C

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Herein, we developed honeycomb-like scaffolds by combining poly (d, l-lactic acid) (PDLLA) with a high amount of graphene/multi-walled carbon nanotube oxides (MWCNTO-GO, 50% w/w). From pristine multi-walled carbon nanotubes (MWCNT) powders, we produced MWCNTO-GO via oxygen plasma etching (OPE), which promoted their exfoliation and oxidation. Initially, we evaluated PDLLA and PDLLA/MWCNTO-GO scaffolds for tensile strength tests, cell adhesion and cell viability (with osteoblast-like MG-63 cells), alkaline phosphatase (ALP, a marker of osteoblast differentiation) activity and mineralized nodule formation. In vivo tests were carried out using PDLLA and PDLLA/MWCNTO-GO scaffolds as fillers for critical defects in the tibia of rats. MWCNTO-GO loading was responsible for decreasing the tensile strength and elongation-at-break of PDLLA scaffolds, although the high mechanical performance observed (~600MPa) assures their application in bone tissue regeneration. In vitro results showed that the scaffolds were not cytotoxic and allowed for osteoblast-like cell interactions and the formation of mineralized matrix nodules. Furthermore, MG-63 cells grown on PDLLA/MWCNTO-GO significantly enhanced osteoblast ALP activity compared to controls (cells alone), while the PDLLA group showed similar ALP activity when compared to controls and PDLLA/MWCNTO-GO. Most impressively, in vivo tests suggested that compared to PDLLA scaffolds, PDLLA/MWCNTO-GO had a superior influence on bone cell activity, promoting greater new bone formation. In summary, the results of this study highlighted that this novel scaffold (MWCNTO-GO, 50% w/w) is a promising alternative for bone tissue regeneration and, thus, should be further studied.

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In vitro and in vivo studies of a novel nanohydroxyapatite/superhydrophilic vertically aligned carbon nanotube nanocomposites

Cited by 18 publications

References 25 publications

Electrospun ultrathin PBAT/nHAp fibers influenced the in vitro and in vivo osteogenesis and improved the mechanical properties of neoformed bone

Electrospun ultrathin PBAT/nHAp fibers influenced the in vitro and in vivo osteogenesis and improved the mechanical properties of neoformed bone

Regenerative nanomedicine: current perspectives and future directions

PDLLA honeycomb-like scaffolds with a high loading of superhydrophilic graphene/multi-walled carbon nanotubes promote osteoblast in vitro functions and guided in vivo bone regeneration

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