In vitro and in vivo anti-inflammatory and anti-arthritic effect of Tinospora cordifolia via modulation of JAK/STAT pathway

George, Genu; Shyni, G. L.; Mohan, Sreelekshmi; Abraham, Billu; Nisha, P.; Sreedharan, Ranjith; Rajankutty, K; Raghu, K.

doi:10.1007/s10787-023-01155-7

Cited by 8 publications

(9 citation statements)

References 58 publications

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“…Hook. f. and Thomson also showed anti-inflammatory effects on lypossacharide-stimulated RAW 264.7 cells by reducing IL-6, TNF-α, PGE2, and nitric oxide levels and iNOS and COX expression via modulation of the JAK/STAT pathway [317]. On the other hand, 100-200 µM blueberry polyphenols can reduce TNF-α, IL-1β, MMP3, and NF-κB levels on HIG-82 rabbit synoviocytes previous stimulated with TNF-α [318].…”

Section: Clinical Trialsmentioning

confidence: 89%

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Potential Role of Dietary Phenolic Compounds in the Prevention and Treatment of Rheumatoid Arthritis: Current Reports

Gonçalves,

Rodrigues,

Fonseca

et al. 2024

Pharmaceuticals

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Rheumatoid arthritis (RA) is a complex illness with both hereditary and environmental components. Globally, in 2019, 18 million people had RA. RA is characterized by persistent inflammation of the synovial membrane that lines the joints, cartilage loss, and bone erosion. Phenolic molecules are the most prevalent secondary metabolites in plants, with a diverse spectrum of biological actions that benefit functional meals and nutraceuticals. These compounds have received a lot of attention recently because they have antioxidant, anti-inflammatory, immunomodulatory, and anti-rheumatoid activity by modulating tumor necrosis factor, mitogen-activated protein kinase, nuclear factor kappa-light-chain-enhancer of activated B cells, and c-Jun N-terminal kinases, as well as other preventative properties. This article discusses dietary polyphenols, their pharmacological properties, and innovative delivery technologies for the treatment of RA, with a focus on their possible biological activities. Nonetheless, commercialization of polyphenols may be achievable only after confirming their safety profile and completing successful clinical trials.

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Section: Clinical Trialsmentioning

confidence: 89%

“…Hook. f. and Thomson reduced erythema, paw edema, hyperplasia, IL-6, TNF-α, IL-17, nitric oxide, and PGE2 levels, as well as phosphorylation of STAT3 and the expression of VEGF [317].…”

Section: In Vivo Studiesmentioning

confidence: 92%

Potential Role of Dietary Phenolic Compounds in the Prevention and Treatment of Rheumatoid Arthritis: Current Reports

Gonçalves,

Rodrigues,

Fonseca

et al. 2024

Pharmaceuticals

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“…ex Maton ethanol extracts have immunomodulatory effects of increasing NO, TNF-α, IL-6, and COX-2 production. 25,35 This study showed that Boesenbergia rotunda (L.) Mansf, Curcuma aeruginosa Roxb., Curcuma zanthorrhiza Roxb., and Zingiber officinale var. Rubra plays a role in reducing NO production in LPS-induced RAW 264.7 cells.…”

Section: Activation Application Of Raw 2647 In Zingiberaceaementioning

confidence: 97%

“…The major adaptor protein of TLR4 is myeloid differentiation primary response gene 88 (MyD88), which leads to downstream signaling. 25 The hydrophobic pocket of myeloid differentiation protein 2 (MD2) directly binds to five lipid chains of LPS associated with TLR4. The homodimer of the TLR4-MD2 complex on the cell surface then recruits central adaptor proteins such as MyD88, which is linked to IL-1 receptor-associated kinase (IRAK)4.…”

Section: Signaling Pathways In Macrophage Activationmentioning

confidence: 99%

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RAW 264.7 Macrophage Cell Line: In Vitro Model for the Evaluation of the Immunomodulatory Activity of Zingiberaceae

2023

TJNPR

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The immune response plays an essential role in the body's defense against infection. Macrophages are promising targets against which to screen agents that modulate immune responses. In vitro analysis, primarily using cell lines, is preferred to screen bioactivity initially. RAW 264.7 cells comprise a model macrophage cell line close to primary murine macrophages. Indonesian people often use the Zingiberaceae family or “empon-empon" in the favorable treatment of several diseases, including immune disorders. This review highlights several studies of Zingiberaceae using RAW 264.7 cells, focusing on the observed immunomodulatory activity and assay methods. The research on RAW 264.7 macrophage cell line in Zingiberaceae was gathered using Scopus, PubMed, ScienceDirect, and Google Scholar from 2012-2021. Based on the reviews, the Zingiberaceae family has immunomodulatory effects by increasing or decreasing inflammatory mediators such as NO, IL-6, TNF-α, IL-1β, IL-10, and phagocytosis activity in RAW 264.7 cells. Several methods can assess the activation of RAW 264.7 cells, including cell viability assays using 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide (MTT); measuring nitric oxide production (Griess reaction), cytokine production (enzyme-linked immunosorbent assay [ELISA]), and phagocytosis (neutral red uptake assay); and detecting DNA (real-time quantitative polymerase chain reaction [qPCR]). The information provided in this review presents avenues for future investigations of the immunomodulatory activity of Zingiberaceae.

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Exploration of Potential Anti‐Inflammatory Cum Anti‐Rheumatoid‐Arthritis Phyto‐Molecule Through Integrated Green Approach: Network Pharmacology, Molecular Docking, Molecular Dynamics, In‐Vitro and Ex‐Vivo Study

Kumar,

Ashif Ikbal,

Ahmed Laskar

et al. 2024

Chemistry & Biodiversity

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Rheumatoid arthritis (RA) and associated inflammatory complications are the most prevalent illnesses and can turn into fatal conditions if left untreated. Allopathic medicine is not satisfactory for curing RA. Scientific literature reports reveal that several phyto‐compounds viz. flavonoids, saponins, and terpenoids, can heal joints and organs from auto‐inflammatory rheumatoid arthritis and pain. Gene ontology, gene network analysis, molecular clustering, and literature review were used to optimise RA‐specific highly expressed genes. In‐silico molecular docking was performed to short‐out potential phytomolecules (Neohesperidin dihydrochalcone (NHDC)) from 1000 datasets‐library against RA and validate using MD simulation running at 100 ns. In‐vitro anti‐inflammatory assays of NHDC inhibited egg‐albumin denaturation, IC50 of 47.739±0.51 μg/ml. The ex‐vivo MTT assay with NHDC rendered 67.209 % inhibition at 100 μM against fd‐FLS‐cells. NHDC downregulated pro‐inflammatory cytokine IL‐17 A production by 61.11 % and 50 % at 300 and 200 μM, respectively. Thus, this Studies recommend that NHDC may be highlighted as a novel multi‐target PADI4 and JAK3 inhibitor with better efficacy and minimal toxicity in RA warranted to In‐Vivo and clinical investigation. The current findings have uncovered remarkable genes and signalling pathways linked to RA, which could enhance our existing comprehension of the molecular mechanisms that drive its development and progression.

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In vitro and in vivo anti-inflammatory and anti-arthritic effect of Tinospora cordifolia via modulation of JAK/STAT pathway

Cited by 8 publications

References 58 publications

Potential Role of Dietary Phenolic Compounds in the Prevention and Treatment of Rheumatoid Arthritis: Current Reports

Potential Role of Dietary Phenolic Compounds in the Prevention and Treatment of Rheumatoid Arthritis: Current Reports

RAW 264.7 Macrophage Cell Line: In Vitro Model for the Evaluation of the Immunomodulatory Activity of Zingiberaceae

Exploration of Potential Anti‐Inflammatory Cum Anti‐Rheumatoid‐Arthritis Phyto‐Molecule Through Integrated Green Approach: Network Pharmacology, Molecular Docking, Molecular Dynamics, In‐Vitro and Ex‐Vivo Study

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