In vitro and in vivo genotoxicity assessment of Cr(VI) using comet assay in earthworm coelomocytes

Manerikar, R. S.; Apte, A. A.; Ghole, Vikram S.

doi:10.1016/j.etap.2007.08.009

Cited by 60 publications

(22 citation statements)

References 24 publications

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“…Genotoxicity of hexavalent chromium only was recorded in an indigenous species of earthworm, Dichogaster curgensis, exposed for 1, 7, or 14 days to soils spiked with potassium dichromate (K 2 Cr 2 O 7 ) at concentrations ranging between 1 and 100 mg Cr(VI)/kg d.w. [Manerikar et al, 2008]. In our study, chromium was in a trivalent form, which had not been shown to be genotoxic and concentrations were within the range of normal values.…”

Section: Discussionmentioning

confidence: 91%

“…The study of coelomocytes in earthworms is relevant because these leukocytes, located around the intestinal tract, are particularly exposed to soil pollutants and are involved in the process of cell immunity [Dhainaut andScaps, 2001, Manerikar et al, 2008]. The comet assay applied to earthworms has been used in previous studies to investigate the genotoxicity of artificial or natural soils spiked with dioxins [Verschaeve et al, 1993], heavy metals [Bierkens et al, 1998;Reinecke and Reinecke, 2004;Zhu et al, 2006;Manerikar et al, 2008], pesticides [Zang et al, 2000;Bustos-Obregón and Goicochea, 2003;Xiao et al, 2006a;Casabé et al, 2007], or PAHs [Martin et al, 2005;Zhu et al, 2006]. Yet, studies dealing with the genotoxicity of field-contaminated soils to earthworms are rare [White and Claxton, 2004].…”

Section: Introductionmentioning

confidence: 99%

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Genotoxic and reproductive effects of an industrially contaminated soil on the earthworm Eisenia Fetida

Bonnard¹,

Eom²,

Morel³

et al. 2008

Environ and Mol Mutagen

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Polluted soil sampled from a former coking plant in Lorraine (France) was studied for its genotoxicity and reproductive effects on the Eisenia fetida earthworm. Genotoxicity was investigated by means of the single-cell gel electrophoresis (comet) assay on the coelomocytes of earthworms after 4 and 10 days of exposure to the soil. DNA damage and a decline in the number of coelomocytes extruded from earthworms were observed at coking plant soil concentrations of 20 and 40% (w/w) in ISO soil. These soil concentrations had previously been shown to significantly reduce cocoon and juvenile productions after 28 and 56 days of earthworm exposure, respectively. The results showed that genotoxic pollutants in the tested soil were still bioavailable despite the age of the contaminated soil. Similar values of the no-observedeffect concentration (NOEC) corresponding to 10% of the contaminated soil and of the lowest soil concentration tested inducing effects (LOEC) corresponding to 20% of the contaminated soil were obtained from reproductive and genotoxicity endpoints. Among the soil pollutants measured, polycyclic aromatic hydrocarbons (PAHs) appeared to be the most likely source of the genotoxicity recorded, although effects of metals could not be excluded. Measurement of genotoxicity in earthworms could complement the existing standardized tests used in the ecotoxicological assessment of the risk associated with contaminated soils. Environ. Mol. Mutagen. 50:60-67, 2009.

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Section: Discussionmentioning

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Genotoxic and reproductive effects of an industrially contaminated soil on the earthworm Eisenia Fetida

Bonnard¹,

Eom²,

Morel³

et al. 2008

Environ and Mol Mutagen

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“…During this reduction process, chromium produces reactive oxygen species and generates oxidative stress. This in turn is responsible for defective hematopoiesis [6]. It has been reported that chromium (VI) is considered as a toxic transition heavy metal and a potent industrial hazard that causes severe damage to a variety of tissues and organs including the reproductive system [7][8][9][10].…”

Section: Introductionmentioning

confidence: 99%

Effect of Sub-Chronic Exposure to Chromium on Haematological and Biochemical Parameters of Male Albino Rat

Saha

Choudhuri

2017

Asian J Pharm Clin Res

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Objectives: Exposure to chromium, the transition element found in many compounds of earth's crust, leads to various health hazards including cancer, dermatitis, damage of liver and kidneys, and reproductive toxicity in both male and female. Toxic effect of chronic exposure to chromium at low environmentally relevant dose is recently recognized and less studied. In the present study, we evaluated the effect of subchronic exposure to low doses of chromium VI (K 2 Cr 2 O 7 ) on various hematological and biochemical parameters in male albino rats. Methods:Male albino rats (160±10 g) received 1/25, 1/50, and 1/100 of reported oral LD 50 dose of chromium VI (K 2 Cr 2 O 7 ) for 90 consecutive days. After treatment, the blood was collected for analysis of various hematological and biochemical parameters.Results: Exposure to chromium VI at 1/25 LD 50 dose caused decrease in body weight gain and reduction in the weight of the reproductive organs along with increase in the weight of the liver and kidney. Numbers of red blood cells, white blood cells, platelets, and hemoglobin concentration reduced with increase in clotting time. The total sperm count and motility was also reduced in exposed rats. There was a decrease in plasma protein and albumin concentration, with increase in plasma glucose and cholesterol level. The activity of enzyme alanine aminotransferase and enzyme aspartate aminotransferase was stimulated in treated rats. Conclusion:The results of the present study revealed that chronic low-dose exposure to chromium VI resulted in a toxic effect in experimental male albino rats.

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“…The Comet assay has been demonstrated to be effective in determining DNA damage levels in the coelomocytes of earthworms exposed to genotoxic compounds, both in vivo and in vitro, in several studies (Reinecke & Reinecke, 2004;Fourie et al, 2007;Di Marzio 2005;Bonnard et al, 2009). Dose-dependent DNA damage in earthworm coelomocytes has been demonstrated in vivo for chromium (Manerikar et al, 2008), cadmium (Fourie et al, 2007) nickel (Reinecke & Reinecke, 2004;Bigorgne et al, 2010) and arsenic (Button et al 2010).…”

Section: Genotoxicity Biomarkersmentioning

confidence: 99%

Earthworm Biomarkers as Tools for Soil Pollution Assessment

Lionetto¹,

Calisi²,

Schettino³

2012

Soil Health and Land Use Management

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In vitro and in vivo genotoxicity assessment of Cr(VI) using comet assay in earthworm coelomocytes

Cited by 60 publications

References 24 publications

Genotoxic and reproductive effects of an industrially contaminated soil on the earthworm Eisenia Fetida

Genotoxic and reproductive effects of an industrially contaminated soil on the earthworm Eisenia Fetida

Effect of Sub-Chronic Exposure to Chromium on Haematological and Biochemical Parameters of Male Albino Rat

Earthworm Biomarkers as Tools for Soil Pollution Assessment

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