In Vitro and in Vivo Effects of Apple Peel Polyphenols against Helicobacter pylori

Abstract

The inhibitory effects of a standarized apple peel polyphenol-rich extract (APPE) against Helicobacter pylori infection and vacuolating bacterial toxin (VacA) induced vacuolation were investigated. Apple peel polyphenols significantly prevented vacuolation in HeLa cells with an IC(50) value of 390 microg of gallic acid equivalents (GAE)/mL. APPE also displayed an in vitro antiadhesive effect against H. pylori. A significant inhibition was observed with a 20-60% reduction of H. pylori attachment at concentrations between 0.250 and 5 mg of GAE/mL. In a short-term infection model (C57BL6/J mice), two levels of APPE doses (150 and 300 mg/kg/day) showed an inhibitory effect on H. pylori attachment. Orally administered apple peel polyphenols also showed an anti-inflammatory effect on H. pylori-associated gastritis, lowering malondialdehyde levels and gastritis scores.

Inhibition of Helicobacter pylori growth and its colonization factors by Parthenium hysterophorus extracts

“…Meanwhile, an apple peel extract showed to inhibit the attachment of H. pylori to HeLa cells by 80% at 10 mg/ml (Pastene et al, 2010).…”

Phytochemicals in Helicobacter pylori Infections: What Are We Doing Now?

“…Pastene et al [101] investigated the inhibitory effects of a standardized apple peel polyphenol-rich extract ( Malus pumila Mill., cited as Malus domestica ) against H. pylori infection and vacuolating bacterial toxin (VacA)-induced vacuolation and found that the preparation significantly prevented vacuolation in HeLa cells with an IC 50 value of 390 μg gallic acid equivalents (GAE)/mL and an in vitro anti-adhesive effect against H. pylori . A significant inhibition was also stated with 20–60% reduction of H. pylori attachment at concentrations between 0.250 and 5 mg GAE/mL.…”

Dietary amelioration of Helicobacter infection

“…The second study, by Gottland and colleagues in Chile, showed that administration of cranberry juice for three weeks inhibited H. pylori about 15% of asymptomatic, colonized children, and that in most subjects who became negative (as measured by UBT), the clearing effect did not persist following cessation of consumption [80]. Furthermore, grape skin/seed extracts and wine preparations [128], (recently reviewed by Friedman [127]), pomegranate fruit and ellagic acid-rich juice (recently reviewed by Colombo [52]), apple [126], and Rubus idaeus and R. occicentalis fruit [154], have all been shown to have antimicrobial activity against H. pylori in vitro . Whereas animal models have been used to demonstrate efficacy of some of these fruit products, no trials currently listed on the USA government website ClinicalTrials.gov, propose to evaluate effects on H. pylori colonization.…”