In-vitro and in-vivo anti-inflammatory action of the ethanol extract of Trachelospermi caulis

Abstract

In this study, we aimed to investigate the anti-inflammatory activity, antinociceptive activity and the action mechanism of Trachelospermi caulis extract. The anti-inflammatory effects were investigated using arachidonic acid, 12-O-tetradecanoylphorbol 13-acetate or carrageenan-induced oedema assays. Antinociceptive activity, using the acetic acid-induced writhing model, was also tested in mice. The extract exhibited dose-dependent and significant (P<0.05 at 100-400 mg kg-1) anti-inflammatory and antinociceptive activity in the animals. To further understand the mechanism of activity, we investigated whether the extract inhibited the expression of inducible nitric oxide synthase (iNOS), the production of nitric oxide (NO) and the expression of TNF-alpha from murine macrophage RAW 264.7 cells. Similar to the in-vivo activity, the iNOS expression, NO production and TNF-alpha expression were found to be dose dependent and significantly suppressed by the extract through the inhibition of the p38 MAP kinase/NF-kappaB pathway. Taken together, the results presented here suggest that T. caulis extract may be useful for the treatment of various inflammatory diseases.

Phytochemistry, Pharmacology and Traditional Uses of Plants from the Genus Trachelospermum L.

“…Among vine plants, T. jasminoides extract (extract/herbs = 0.85 g/30 g) exhibited significant anti-inflammatory ability against COX-1, COX-2, PLA 2 and 12-LO with the IC 50 values of 35, 138, 33 and 29 μg/mL respectively [51], which implied that T. jasminoides could prevent the generation of inflammatory mediators from arachidonic acid metabolism. Besides, the extract restrained the LPS-induced expression of inducible nitric oxide synthase (iNOS) protein and tumor necrosis factor-α (TNF- α ) in RAW 264.7 cells in a dose-dependent manner (30–300 mg/mL) [52]. Meanwhile, the activating phosphorylations of p38 MAP kinase and NF-kB were inhibited by the extract in a dose-dependent manner, which were important for iNOS expression and NO production.…”

“…The extract suppressed carrageenan-induced oedema in a dose-dependent and time-dependent manner in rats compared with the control groups [52]. …”

Cytotoxic activities against MCF-7 and MDA-MB-231, antioxidant andα-glucosidase inhibitory activities ofTrachelospermum jasminoidesextractsin vitro

“…T. jasminoides, a member of the family Apocynaceae, is also known as luoshiteng in China and suppresses some inflammatory diseases such as rheumatoid arthritis and abscesses [9][10][11][12] and shows anti-cancer and antibacterial activity [13][14][15]. The anti-breast cancer activity of the extracts of T. jasminoides along with its antioxidant and a-glucosidase inhibitory activities have been poorly reported so far and were explored and reported in this research.…”

Simultaneous quantification of tracheloside and trachelogenin in rat plasma using liquid chromatography/tandem mass spectrometry

“…T. jasminoides has been shown to exhibit anti-inflammatory and anti-cancer properties, among others [2][3][4][5]. In advanced pharmacological studies, the total lignan extract from T. jasminoides showed significant anti-inflammatory and analgesic effects [6].…”