In vitro and in vivo characterization of graphene oxide coated porcine bone granules

Ettorre, Valeria; Marco, Patrizia De; Zara, Susi; Perrotti, Vittoria; Crescenzo, Antonello Di; Petrini, Morena; Hadad, Caroline; Bosco, Domenico; Zavan, Barbara; Valbonetti, Luca; Spoto, Giuseppe; Iezzi, Giovanna; Piattelli, Adriano; Cataldi, Amelia; Fontana, Antonella

doi:10.1016/j.carbon.2016.03.010

Cited by 45 publications

(44 citation statements)

References 31 publications

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“…The GO contains several functional groups C = O, C = C (C-C), O-C = O, O-H1729, and Zr(OC 3 H 7 ) 4 have -C(CH3) 2 , -CH 3 , C = C, C-O-Zr, Zr-O groups1930. For RGO-Zr, new peaks appeared at the bending vibrations of O-H groups mixed with Zr-OH bending31, the C = C skeletal vibration of the RGO sheets32, -CH 2 symmetrical stretching vibrations, and -CH 2 antisymmetric stretching vibration.…”

Section: Resultsmentioning

confidence: 99%

Adsorption of phosphate in water using one-step synthesized zirconium-loaded reduced graphene oxide

Luo

Wang

Shu

et al. 2016

Sci Rep

101

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In this account, a one-step green hydrothermal method for zirconium-loaded reduced graphene oxide (RGO-Zr) adsorbent was developed in pure water. It is based on the formation of initially strong-coupling RGO-Zr nanocomposites followed by in situ reduction of GO to RGO during the hydrothermal treatment. The phosphate adsorption performance of the as-prepared nanocomposites was investigated in aqueous environment under various conditions. The characterization results of RGO-Zr nanocomposites showed that ZrO2 was successfully integrated onto the RGO sheets in amorphous. The data from equilibrium phosphate adsorption on RGO-Zr revealed that the adsorption kinetics followed a pseudo-second-order kinetic model, where the adsorption isotherm fitted the Langmuir isotherm model with a maximum adsorption capacity of 27.71 mg P/g at pH 5 and 298 K. The improved phosphate adsorption on RGO-Zr was caused by the dispersion of ZrO2 on the RGO surface. Furthermore, the phosphate adsorption was found insensitive to the increase in pH while it was sensitive to the increase in temperature. The coexisting anions of SO42−, F−, Cl−, NO3− and CO32− affected the phosphate adsorption in a different way. Results suggest that the present RGO-Zr adsorbent has the potential for controlling phosphorus pollution in water.

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Section: Resultsmentioning

confidence: 99%

Adsorption of phosphate in water using one-step synthesized zirconium-loaded reduced graphene oxide

Luo

Wang

Shu

et al. 2016

Sci Rep

101

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“…All of these features, gathered in one material, confer on graphene characteristics of high interest for meaningful applications. Graphene has therefore been exploited in different fields and applications, ranging from optoelectronics and highenergy physics to material science and medicine (11)(12)(13)(14). One drawback of graphene is its low solubility in both organic and aqueous solvents.…”

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confidence: 99%

Antimicrobial and Antibiofilm Efficacy of Graphene Oxide against Chronic Wound Microorganisms

Giulio

Zappacosta

Lodovico

et al. 2018

Antimicrob Agents Chemother

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125

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Chronic wounds represent an increasing problem worldwide. Graphene oxide (GO) has been reported to exhibit strong antibacterial activity toward both Gram-positive and Gram-negative bacteria. The aim of this work was to investigate the antimicrobial and antibiofilm efficacy of GO against wound pathogens. PECHA 10, PECHA 4, and X3 clinical isolates were incubated with 50 mg/liter of GO for 2 and 24 h to evaluate the antimicrobial effect. Optical and atomic force microscopy images were performed to visualize the effect of GO on microbial cells. Moreover, the antibiofilm effect of GO was tested on biofilms, both in formation and mature. Compared to the respective time controls, GO significantly reduced the growth both at 2 and 24 h in a time-dependent way, and it displayed a bacteriostatic effect in respect to the GO = 0; an immediate (after 2 h) slowdown of bacterial growth was detected for, whereas a tardive effect (after 24 h) was recorded for Atomic force microscopy images showed the complete wrapping of and with GO sheets, which explains its antimicrobial activity. Moreover, significant inhibition of biofilm formation and a reduction of mature biofilm were recorded for each detected microorganism. The antibacterial and antibiofilm properties of GO against chronic wound microorganisms make it an interesting candidate to incorporate into wound bandages to treat and/or prevent microbial infections.

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“…The idea to covalently functionalize the Ti discs, rather than simply physically load GO on Ti surfaces, derives from the aim of preparing a material that did not leak GO in the neighboring tissue. As a matter of fact, in a previous study on porcine bone granules physically coated with GO, aggregated GO were evidenced in the soft tissue after three months deposition in the animal, despite that the same material did not show any evidences of GO leakage after seven days of dip in aqueous solution or PBS [20]. The covalent functionalization ensures that excess GO, not properly bound to the surface, is washed out from the material.…”

Section: Discussionmentioning

confidence: 90%

Osteoblastic Differentiation on Graphene Oxide-Functionalized Titanium Surfaces: An In Vitro Study

et al. 2020

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Background: Titanium implant surfaces are continuously modified to improve biocompatibility and to promote osteointegration. Graphene oxide (GO) has been successfully used to ameliorate biomaterial performances, in terms of implant integration with host tissue. The aim of this study is to evaluate the Dental Pulp Stem Cells (DPSCs) viability, cytotoxic response, and osteogenic differentiation capability in the presence of GO-coated titanium surfaces. Methods: Two titanium discs types, machined (control, Crtl) and sandblasted and acid-etched (test, Test) discs, were covalently functionalized with GO. The ability of the GO-functionalized substrates to allow the proliferation and differentiation of DPSCs, as well as their cytotoxic potential, were assessed. Results: The functionalization procedures provide a homogeneous coating with GO of the titanium surface in both control and test substrates, with unchanged surface roughness with respect to the untreated surfaces. All samples show the deposition of extracellular matrix, more pronounced in the test and GO-functionalized test discs. GO-functionalized test samples evidenced a significant viability, with no cytotoxic response and a remarkable early stage proliferation of DPSCs cells, followed by their successful differentiation into osteoblasts. Conclusions: The described protocol of GO-functionalization provides a novel not cytotoxic biomaterial that is able to stimulate cell viability and that better and more quickly induces osteogenic differentiation with respect to simple titanium discs. Our findings pave the way to exploit this GO-functionalization protocol for the production of novel dental implant materials that display improved integration with the host tissue.

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In vitro and in vivo characterization of graphene oxide coated porcine bone granules

Cited by 45 publications

References 31 publications

Adsorption of phosphate in water using one-step synthesized zirconium-loaded reduced graphene oxide

Adsorption of phosphate in water using one-step synthesized zirconium-loaded reduced graphene oxide

Antimicrobial and Antibiofilm Efficacy of Graphene Oxide against Chronic Wound Microorganisms

Osteoblastic Differentiation on Graphene Oxide-Functionalized Titanium Surfaces: An In Vitro Study

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