In Vitro and In Vivo Antimalarial Activity of LZ1, a Peptide Derived from Snake Cathelicidin

Fang, Yaqun; He, Xiaoqin; Zhang, Pengcheng; Shen, Chuanbin; Mwangi, James; Xu, Cheng; Mo, Guoxiang; Lai, Ren; Zhang, Zhiye

doi:10.3390/toxins11070379

Cited by 31 publications

(21 citation statements)

References 42 publications

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“…A positive control groups treated with chloroquine diphosphate at a daily dose of 25 mg/kg/body all survived up to 21 days, indicating that the infected mice were completely cured of Pb ANKA. This is consistent with the results of Fang et al [22] Although many therapeutic antimalaria have been reported, insu cient e cacy has been a critical issue in treating malaria. Also, antimalarial drug resistance is a major hurdle.…”

Section: Resultssupporting

confidence: 92%

Antiplasmodial Potential of Indonesian Medicinal Plants

Bialangi¹,

Mustapa²,

Salimi³

et al. 2020

Preprint

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Background: Species A. paniculata (Burm. f.) Nees known as″ Sambiloto ″ and P. pellucida L. Kunth known as″ Suruhan ″ are mainly distributed in Indonesia and their combination was used as a traditional medicine for treating malaria diseases. However, no information appears to have evaluated the antiplasmodial potential of the two plants. This research aimed to evaluate the antiplasmodial activity of the two plants and the species P. pellucida L. Kunth alone as a source of antiplasmodial agent. Methods: In vitro test of the AP-PP and PP extracts against Pf D-10 (chloroquine-sensitive) were performed as described by Desjardins et al. An in vivo test of the PP extract in mice infected with Pb ANKA was performed using Peters´ 4-day suppressive test. Parasitemia, growth and inhibition rates were determined via Giemsa-stained smear of blood and analyzed microscopically. Survival was followed up until day 21 post-infection.Results: The increased ratio of the PP extract (20:80) exhibited significant antiplasmodial in contrast to the high ratio of the AP extract (IC50, 62.01 mg/mL). Further evaluation of the PP extract alone displayed better antiplasmodial activity with an IC50 value of 4.0 mg/mL. Furthermore, an in vivo test of the PP extract in BALB/c albino mice infected with Pb ANKA exhibited a significant chemosuppressive effect in a dose-dependent manner.Conclusion: The increased ratio of the PP extract exhibited a major contribution for their activity. The PP extract alone showed better antiplasmodial activity than the AP extract and their combination. An in vivo test confirmed the efficacy of the PP extract in mouse model.

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Section: Resultssupporting

confidence: 92%

Antiplasmodial Potential of Indonesian Medicinal Plants

Bialangi¹,

Mustapa²,

Salimi³

et al. 2020

Preprint

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“…Fang et al described the antimalarial activity of LZ1 (IC 50 value of 3.045 μM against P. falciparum ), 41 a peptide derived from snake cathelicidin. Decoralin, a wasp venom peptide, did not present antiplasmodial activity against P. gallinaceum ; however, a number of Arg‐substituted analogs showed antiplasmodial activity at 60 μmol L −1 42 .…”

Section: Resultsmentioning

confidence: 99%

Net charge tuning modulates the antiplasmodial and anticancer properties of peptides derived from scorpion venom

Pedron

Silva

Torres

et al. 2021

Journal of Peptide Science

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VmCT1, a linear helical antimicrobial peptide isolated from the venom of the scorpion Vaejovis mexicanus, displays broad spectrum antimicrobial activity against bacteria, fungi, and protozoa. Analogs derived from this peptide containing single Arg‐substitutions have been shown to increase antimicrobial and antiparasitic activities against Trypanossoma cruzi. Here, we tested these analogs against malaria, an infectious disease caused by Plasmodium protozoa, and assessed their antitumoral properties. Specifically, we tested VmCT1 synthetic variants [Arg]3‐VmCT1‐NH2, [Arg]7‐VmCT1‐NH2, and [Arg]11‐VmCT1‐NH2, against Plasmodium gallinaceum sporozoites and MCF‐7 mammary cancer cells. Our screen identified peptides [Arg]3‐VmCT1‐NH2 and [Arg]7‐VmCT1‐NH2 as potent antiplasmodial agents (IC50 of 0.57 and 0.51 μmol L−1, respectively), whereas [Arg]11‐VmCT1‐NH2 did not show activity against P. gallinaceum sporozoites. Interestingly, all peptides presented activity against MCF‐7 and displayed lower cytotoxicity toward healthy cells. We demonstrate that increasing the net positive charge of VmCT1, through arginine substitutions, modulates the biological properties of this peptide family yielding novel antiplasmodial and antitumoral molecules.

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“…Our previous work established that BF-30 and its analogs show effective broad-spectrum antimicrobial abilities and therapeutic potential for pancreatic cancer ( Wang et al, 2011 ; Jin et al, 2016 ; Fang et al, 2019 ; Xu C. et al, 2019 ). In this study, we found that ZY13 exhibits remarkable anti-ZIKV efficacy in vitro with IC 50 of 1.06 ± 0.13 μM in U251 cells and IC 50 of 1.81 ± 0.34 μM in Vero cells.…”

Section: Discussionmentioning

confidence: 99%

Snake Cathelicidin Derived Peptide Inhibits Zika Virus Infection

Xing

Jie

et al. 2020

Front. Microbiol.

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Zika virus (ZIKV) is a mosquito-borne virus belonging to the genus Flavivirus and has reemerged in recent years with epidemic potential. ZIKV infection may result in severe syndromes such as neurological complications and microcephaly in newborns. Therefore, ZIKV has become a global public health threat and currently there is no approved specific drug for its treatment. Animal venoms are important resources of novel drugs. Cathelicidin-BF (BF-30) is a defensive peptide identified from Bungarus fasciatus snake venom and has been shown to be an excellent template for applicable peptide design. In this study, we found that ZY13, one of the peptidic analogs of BF-30, inhibits ZIKV infection in vitro and in vivo. Mechanistic studies revealed that ZY13 can directly inactivate ZIKV and reduce the production of infectious virions. Further studies also indicated that administration of ZY13 strengthen the host antiviral immunity via AXL-SOCS (suppressor of cytokine signaling protein) pathway. Additionally, the results of mouse experiment suggest that ZY13 efficiently restrict ZIKV infection and improve the growth defects of ZIKV-infected mouse pups. Together, our findings not only demonstrate that ZY13 might be a candidate for anti-ZIKV drug, but also indicated the importance of animal venom peptides as templates for antivirals development.

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In Vitro and In Vivo Antimalarial Activity of LZ1, a Peptide Derived from Snake Cathelicidin

Cited by 31 publications

References 42 publications

Antiplasmodial Potential of Indonesian Medicinal Plants

Antiplasmodial Potential of Indonesian Medicinal Plants

Net charge tuning modulates the antiplasmodial and anticancer properties of peptides derived from scorpion venom

Snake Cathelicidin Derived Peptide Inhibits Zika Virus Infection

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