In Vitro and in Vivo Wound Healing Properties of Plasma and Serum from Crocodylus siamensis Blood

Abstract

The plasma and serum of Crocodylus siamensis have previously been reported to exhibit potent antimicrobial, antioxidant, and anti-inflammatory activities. During wound healing, these biological properties play a crucial role for supporting the formation of new tissue around the injured skin in the recovery process. Thus, this study aimed to evaluate the wound healing properties of C. siamensis plasma and serum. The collected data demonstrate that crocodile plasma and serum were able to activate in vitro proliferation and migration of HaCaT, a human keratinocyte cell line, which represents an essential phase in the wound healing process. With respect to investigating cell migration, a scratch wound experiment was performed which revealed the ability of plasma and serum to decrease the gap of wounds in a dose-dependent manner. Consistent with the in vitro results, remarkably enhanced wound repair was also observed in a mouse excisional skin wound model after treatment with plasma or serum. The effects of C. siamensis plasma and serum on wound healing were further elucidated by treating wound infections by Staphylococcus aureus ATCC 25923 on mice skin coupled with a histological method. The results indicate that crocodile plasma and serum promote the prevention of wound infection and boost the re-epithelialization necessary for the formation of new skin. Therefore, this work represents the first study to demonstrate the efficiency of C. siamensis plasma and serum with respect to their wound healing properties and strongly supports the utilization of C. siamensis plasma and serum as therapeutic products for injured skin treatment.

In Vivo Wound Healing Activity of Crocodile (Crocodylus siamensis) Hemoglobin and Evaluation of Antibacterial and Antioxidant Properties of Hemoglobin and Hemoglobin Hydrolysate

“…The experiment was performed according to the method of Jangpromma et al [4] and Lodhi et al [22] with some modifications. Female ICR mice (n = 12) were randomly divided into four groups, including three treated groups and one control group (no treatment).…”

“…family, particularly focusing on C. siamensis, linked crocodile plasma and serum with antibacterial properties, as well as with an improvement of wound healing in mice models [4]. In addition, a leukocyte extract of C. siamensis was shown to exhibit antibacterial activity against Staphylococcus epidermidis, Salmonella typhi, and Vibrio cholerae [5].…”

Nanosilver coated Chitin derived membrane as an Eco-friendly Dressing and Its Effect on Wound Healing

“…For compact skin species (e.g. human beings), the wound healing mainly depends on the re-epithelialization of the wound surface [17]. There was no difference in the length of neonatal epithelium in each group after 3 days of injury.…”

Preparation of a balsa-lysozyme eco-friendly dressing and its effect on wound healing

“…For compactskin species such as humans, wound healing is mainly driven by re-epithelialization. 30 There was no difference in the length of new epithelium in the groups aer 3 days of injury (p > 0.05), mainly because the margins were still in the preparation period of cell proliferation and migration aer 3 days of injury, so new epithelium was not obvious. 31 The results for wound length suggest that high concentrations of lysozyme might accelerate the healing of the wound by controlling infection and promoting re-epithelialization in the late post-injury period (7 days aer injury, Fig.…”