In vitro and in vivo studies of the Interferon-alpha action on distinct Orthobunyavirus

Livonesi, Márcia Cristina; Sousa, Ricardo Luiz Moro de; Badra, Soraya Jabur; Figueiredo, Luíz Tadeu Moraes

doi:10.1016/j.antiviral.2007.01.158

Cited by 22 publications

(15 citation statements)

References 35 publications

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“…6A). These findings are consistent with previously published work demonstrating a resistance of OROV to the antiviral effects of IFN-␣ both in vivo and in vitro in comparison to other pathogenic orthobunyaviruses (68). The reasons for the differences in relative sensitivity of OROV and BUNV to IFN-␣ are under investigation, but the differences may, for example, be due to the differential effects of certain ISGs on these viruses or on the ability of OROV to more rapidly switch off host cell gene expression than BUNV.…”

Section: Discussionsupporting

confidence: 83%

Generation of Recombinant Oropouche Viruses Lacking the Nonstructural Protein NSm or NSs

Tilston-Lunel

Acrani

Randall

et al. 2016

J Virol

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Oropouche virus (OROV) is a midge-borne human pathogen with a geographic distribution in South America. OROV was first isolated in 1955, and since then, it has been known to cause recurring outbreaks of a dengue-like illness in the Amazonian regions of Brazil. OROV, however, remains one of the most poorly understood emerging viral zoonoses. Here we describe the successful recovery of infectious OROV entirely from cDNA copies of its genome and generation of OROV mutant viruses lacking either the NSm or the NSs coding region. Characterization of the recombinant viruses carried out in vitro demonstrated that the NSs protein of OROV is an interferon (IFN) antagonist as in other NSs-encoding bunyaviruses. Additionally, we demonstrate the importance of the nine C-terminal amino acids of OROV NSs in IFN antagonistic activity. OROV was also found to be sensitive to IFN-␣ when cells were pretreated; however, the virus was still capable of replicating at doses as high as 10,000 U/ml of IFN-␣, in contrast to the family prototype BUNV. We found that OROV lacking the NSm protein displayed characteristics similar to those of the wild-type virus, suggesting that the NSm protein is dispensable for virus replication in the mammalian and mosquito cell lines that were tested. IMPORTANCE Oropouche virus (OROV) is a public health threat in Central andSouth America, where it causes periodic outbreaks of denguelike illness. In Brazil, OROV is the second most frequent cause of arboviral febrile illness after dengue virus, and with the current rates of urban expansion, more cases of this emerging viral zoonosis could occur. To better understand the molecular biology of OROV, we have successfully rescued the virus along with mutants. We have established that the C terminus of the NSs protein is important in interferon antagonism and that the NSm protein is dispensable for virus replication in cell culture. The tools described in this paper are important in terms of understanding this important yet neglected human pathogen.

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Section: Discussionsupporting

confidence: 83%

Generation of Recombinant Oropouche Viruses Lacking the Nonstructural Protein NSm or NSs

Tilston-Lunel

Acrani

Randall

et al. 2016

J Virol

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“…In the existing literature, data on in vitro or in vivo experiments for potential treatment or prevention options are scanty. A study by Livonesi et al (2006) on ribavirin, a broad-spectrum antiviral drug, reports no inhibitory effect on OROV and another study by Livonesi et al (2007) reports some antiviral activity of IFN-α on OROV [ 78 , 79 ]. Concerning prevention options, so far, there is no vaccine for OROV fever prophylaxis in humans.…”

Section: Treatment and Prevention Optionsmentioning

confidence: 99%

Oropouche Fever: A Review

Sakkas

Bozidis

Franks

et al. 2018

Viruses

141

137

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Oropouche fever is an emerging zoonotic disease caused by Oropouche virus (OROV), an arthropod transmitted Orthobunyavirus circulating in South and Central America. During the last 60 years, more than 30 epidemics and over half a million clinical cases attributed to OROV infection have been reported in Brazil, Peru, Panama, Trinidad and Tobago. OROV fever is considered the second most frequent arboviral febrile disease in Brazil after dengue fever. OROV is transmitted through both urban and sylvatic transmission cycles, with the primary vector in the urban cycle being the anthropophilic biting midge Culicoides paraensis. Currently, there is no evidence of direct human-to-human OROV transmission. OROV fever is usually either undiagnosed due to its mild, self-limited manifestations or misdiagnosed because its clinical characteristics are similar to dengue, chikungunya, Zika and yellow fever, including malaria as well. At present, there is no specific antiviral treatment, and in the absence of a vaccine for effective prophylaxis of human populations in endemic areas, the disease prevention relies solely on vector control strategies and personal protection measures. OROV fever is considered to have the potential to spread across the American continent and under favorable climatic conditions may expand its geographic distribution to other continents. In view of OROV’s emergence, increased interest for formerly neglected tropical diseases and within the One Health concept, the existing knowledge and gaps of knowledge on OROV fever are reviewed.

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“…The virus was originally isolated from a sentinel capuchin monkey (Cebus apella) and subsequently from Anopheles mosquito, and serological surveys showed antibodies in humans, horses, cattle, pigs, bats, rodents, and birds in Brazil and other South American countries (2,11,18). Livonesi et al (22) investigated the effect of IFN-␣ on TCMV replication in vitro and in vivo. Like other tested orthobunyaviruses, TCMV replication was completely inhibited if IFN was applied to cells 24 h before or 2 h after infection.…”

Section: Absence Of An Nss Overlapping Orfmentioning

confidence: 99%

Viruses in the Anopheles A, Anopheles B, and Tete Serogroups in the Orthobunyavirus Genus (Family Bunyaviridae ) Do Not Encode an NSs Protein

Mohamed

McLees

Elliott

2009

J Virol

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In vitro and in vivo studies of the Interferon-alpha action on distinct Orthobunyavirus

Cited by 22 publications

References 35 publications

Generation of Recombinant Oropouche Viruses Lacking the Nonstructural Protein NSm or NSs

Generation of Recombinant Oropouche Viruses Lacking the Nonstructural Protein NSm or NSs

Oropouche Fever: A Review

Viruses in the Anopheles A, Anopheles B, and Tete Serogroups in the Orthobunyavirus Genus (Family Bunyaviridae ) Do Not Encode an NSs Protein

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