In Vitro and in Vivo Antifungal Activities of the Eight Steroid Saponins from Tribulus terrestris L. with Potent Activity against Fluconazole-Resistant Fungal

Zhang, Jundong; Cao, Yongbing; Xu, Zheng; Sun, Huihua; An, Mao Mao; Yan, Lan; Chen, Haisheng; Gao, Ping; Wang, Yan; Jia, Xin Ming; Jiang, Yuan

doi:10.1248/bpb.28.2211

Cited by 59 publications

(37 citation statements)

References 27 publications

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“…The ergosterol biosynthetic pathway is a key target for chemical control of fungal pathogens of plants and animals, and there is constant pressure to identify new compounds with novel modes of action to combat the development of resistance to existing fungicides (14,34,48). The major fungicides that target sterol biosynthesis are azoles (Erg11p), morpholines (Erg24p, Erg2p, and Erg5p), and allylamines (Erg1p).…”

Section: Discussionmentioning

confidence: 99%

Dual Effects of Plant Steroidal Alkaloids on Saccharomycescerevisiae

Simons

Morrissey

Latijnhouwers³

et al. 2006

Antimicrob Agents Chemother

106

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Many plant species accumulate sterols and triterpenes as antimicrobial glycosides. These secondary metabolites (saponins) provide built-in chemical protection against pest and pathogen attack and can also influence induced defense responses. In addition, they have a variety of important pharmacological properties, including anticancer activity. The biological mechanisms underpinning the varied and diverse effects of saponins on microbes, plants, and animals are only poorly understood despite the ecological and pharmaceutical importance of this major class of plant secondary metabolites. Here we have exploited budding yeast (Saccharomyces cerevisiae) to investigate the effects of saponins on eukaryotic cells. The tomato steroidal glycoalkaloid ␣-tomatine has antifungal activity towards yeast, and this activity is associated with membrane permeabilization. Removal of a single sugar from the tetrasaccharide chain of ␣-tomatine results in a substantial reduction in antimicrobial activity. Surprisingly, the complete loss of sugars leads to enhanced antifungal activity. Experiments with ␣-tomatine and its aglycone tomatidine indicate that the mode of action of tomatidine towards yeast is distinct from that of ␣-tomatine and does not involve membrane permeabilization. Investigation of the effects of tomatidine on yeast by gene expression and sterol analysis indicate that tomatidine inhibits ergosterol biosynthesis. Tomatidine-treated cells accumulate zymosterol rather than ergosterol, which is consistent with inhibition of the sterol C 24 methyltransferase Erg6p. However, erg6 and erg3 mutants (but not erg2 mutants) have enhanced resistance to tomatidine, suggesting a complex interaction of erg mutations, sterol content, and tomatidine resistance.Plants produce a vast array of structurally diverse secondary metabolites. These natural products serve as attractants for agents that mediate pollination and seed dispersal; they also provide chemical defenses against pests, pathogens, and invasion by neighboring plants (47). Small molecules therefore play key roles in ecological interactions between plants and other organisms. We exploit the rich reservoir of metabolic diversity provided to us by diverse plant species in order to find new drugs and other valuable compounds. The chemical "space," in terms of the number and variety of molecules produced by plants, is enormous. Structures of over 100,000 diverse compounds have been reported so far (11), and this is inevitably just the tip of the iceberg. However, with a few well-characterized exceptions, we know very little about the biological properties of plant secondary metabolites. Characterization of the biological activities of these compounds will be critical, both from an ecological perspective and a pharmaceutical perspective.The terpenes are one of the largest and most diverse groups of plant secondary metabolites (9). They include sterols and triterpenes, complex compounds that are formed by the cyclization of 2,3-oxidosqualene. Sterols and triterpenes can accumulat...

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Section: Discussionmentioning

confidence: 99%

Dual Effects of Plant Steroidal Alkaloids on Saccharomycescerevisiae

Simons

Morrissey

Latijnhouwers³

et al. 2006

Antimicrob Agents Chemother

106

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“…In agar diffusion method, the plant extract of all the different parts exhibited a great activity against all the bacterial strains 24 . Another study 57 evaluate the antifungal activity of eight isolated steroidal saponins from T. terrestris which is shortened as the following: TTS-8, TTS-9, TTS-10, TTS-11, TTS-12, TTS-13, TTS-14, and TTS-15. The eight isolated saponins were identified as listed in the Table 1.…”

Section: Tribulus Terrestrismentioning

confidence: 99%

Ethnomedicine, phytochemistry and pharmacology of Calotropis procera and Tribulus terrestris

Dirir

Cheruth²,

Ksiksi³

2017

Journal of Natural Remedies

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“…Traditionally, Tribulus terrestris L. is a medicinal plant containing numerous steroidal saponins 9 and has also been reported for treatment of various ailments 10 .The plant extract is used for urinary dysfunction 11,12 and have also shown to have antihypertensive and vasodilatory properties 13 . It exhibits anti oxidant, antitumor, antifungal and anti-helmenthic properties [14][15][16][17] . These properties have created interest for studying the diuretic activity by underlying the exerting mechanism of T. terrestris L. for selecting it as a source.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Physico Chemical Properties and Diuretic Activity of Biotite Calx (Abhraka Bhasma) Processed With Tribulus Terrestris L.: An Experimental Study

Kumar¹,

Ilango²,

Kumar³

et al. 2015

Int. J. Res. Ayurveda Pharm.

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Ayurvedic formulations have been used for centuries with claimed safety and efficacy. The aim of the present study was to evaluate the diuretic effect of Gokshura Kwatha Marita Abhraka Bhasma (biotite calx incinerated with the decoction of Tribulus terrestris L.) in experimental animals. The animals were randomly selected and divided into three groups, each comprising 8 animals. The diuretic activity of Abhraka Bhasma (biotite calx) was evaluated by determining volume, pH and electrolyte concentration of urine in male albino rats at a single dose of 4.5 mg for 200g body weight. Furosemide was used as reference standard and normal saline was used as normal control. Abhraka Bhasma treated rats have shown significant increase in the volume of urine and urinary electrolyte concentrations of Na + , K + and Cl -ions in comparison to normal control. Thus, these results indicate that Abhraka Bhasma processed with the decoction of Tribulus terrestris L. could have potent diuretic activity.

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In Vitro and in Vivo Antifungal Activities of the Eight Steroid Saponins from Tribulus terrestris L. with Potent Activity against Fluconazole-Resistant Fungal

Cited by 59 publications

References 27 publications

Dual Effects of Plant Steroidal Alkaloids on Saccharomycescerevisiae

Dual Effects of Plant Steroidal Alkaloids on Saccharomycescerevisiae

Ethnomedicine, phytochemistry and pharmacology of Calotropis procera and Tribulus terrestris

Evaluation of Physico Chemical Properties and Diuretic Activity of Biotite Calx (Abhraka Bhasma) Processed With Tribulus Terrestris L.: An Experimental Study

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