In vitro and in vivo evaluation of therapy targeting epithelial-cell adhesion-molecule aptamers for non-small cell lung cancer

Alibolandi, Mona; Ramezani, Mohammad; Abnous, Khalil; Sadeghi, Fatemeh; Atyabi, Fatemeh; Asouri, Mohsen; Ahmadi, Ali Asghar; Hadizadeh, Farzin

doi:10.1016/j.jconrel.2015.04.026

Cited by 126 publications

(48 citation statements)

References 57 publications

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“…As such, polymersomes composed of poly(ethylene glycol)- block -poly(lactide- co -glycolide) was used to load doxorubicin via thin film rehydration of the polymer followed by loading of the drugs. [91] The polymersomes, which contained carboxylic acid functional groups, were covalently linked to the EpCAM aptamer with terminal amine functional group via aqueous carbodiimide chemistry. According to electron microscopy images, EpCAM aptamers coupled to the polymersomes caused a 10 nm increase in the particle diameter.…”

Section: Surface Activities Of Polymersomesmentioning

confidence: 99%

Engineering Polymersomes for Diagnostics and Therapy

Leong

Teo

Aakalu

et al. 2018

Adv Healthcare Materials

113

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Engineered polymer vesicles, termed as polymersomes, confer a flexibility to control their structure, properties, and functionality. Self-assembly of amphiphilic copolymers leads to vesicles consisting of a hydrophobic bilayer membrane and hydrophilic core, each of which is loaded with a wide array of small and large molecules of interests. As such, polymersomes are increasingly being studied as carriers of imaging probes and therapeutic drugs. Effective delivery of polymersomes necessitates careful design of polymersomes. Therefore, this review article discusses the design strategies of polymersomes developed for enhanced transport and efficacy of imaging probes and therapeutic drugs. In particular, the article focuses on overviewing technologies to regulate the size, structure, shape, surface activity, and stimuli- responsiveness of polymersomes and discussing the extent to which these properties and structure of polymersomes influence the efficacy of cargo molecules. Taken together with future considerations, this article will serve to improve the controllability of polymersome functions and accelerate the use of polymersomes in biomedical applications.

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Section: Surface Activities Of Polymersomesmentioning

confidence: 99%

Engineering Polymersomes for Diagnostics and Therapy

Leong

Teo

Aakalu

et al. 2018

Adv Healthcare Materials

113

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“…[14][15][16][17][18] Simultaneously, the aptamer as an element of the targeted probe can avoid immunogenicity and its high purity can also guarantee its non-toxicity and safety compared with other ligands. [19][20][21] Early aptamers including A10 and A9 were screened from the synthesized RNA aptamer library, and can both effectively bind to PSMA. 22,23 However, they both consist of 79 nucleotides, which leads to a very high molecular weight and severely restricts their role as targeting molecules.…”

Section: Introductionmentioning

confidence: 99%

Diagnosis of prostate cancer using anti-PSMA aptamer A10-3.2-oriented lipid nanobubbles

Fan

Guo

Wang

et al. 2016

IJN

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In this study, the lipid targeted nanobubble carrying the A10-3.2 aptamer against prostate specific membrane antigen was fabricated, and its effect in the ultrasound imaging of prostate cancer was investigated. Materials including 2-dipalmitoyl-sn-glycero-3-phosphocholine, 1,2-dipalmitoyl-sn-glycero-3-phosphatidic acid, 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine, 1,2-dipalmitoyl-sn-glycero-3-phosphoglycerol, carboxyl-modified 1,2-distearoyl-sn-glycero-3-phosphoethanolamine, and polyethyleneglycol-2000 were for mechanical oscillation, and nanobubbles were obtained through the centrifugal flotation method. After mice were injected with nanobubbles, abdominal color Doppler blood flow imaging significantly improved. Through left ventricular perfusion with normal saline to empty the circulating nanobubbles, nanobubbles still existed in tumor tissue sections, which demonstrated that nanobubbles could enter tissue spaces via the permeability and retention effect. Fluorinated A10-3.2 aptamers obtained by chemical synthesis had good specificity for PSMA-positive cells, and were linked with carboxyl-modified 1,2-distearoyl-sn-glycero-3-phosphoethanolamine lipid molecules from the outer shell of nanobubbles via amide reaction to construct targeted nanobubbles. Gel electrophoresis and immunofluorescence confirmed that targeted nanobubbles were fabricated successfully. Next, targeted nanobubbles could bind with PSMA-positive cells (C4-2 cells), while not with PSMA-negative cells (PC-3 cells), using in vitro binding experiments and flow cytometry at the cellular level. Finally, C4-2 and PC-3 xenografts in mice were used to observe changes in parameters of targeted and non-targeted nanobubbles in the contrast-enhanced ultrasound mode, and the distribution of Cy5.5-labeled targeted nanobubbles in fluorescent imaging of live small animals. Comparison of ultrasound indicators between targeted and non-targeted nanobubbles in C4-2 xenografts showed that they had similar peak times ( P >0.05), while the peak intensity, half time of peak intensity, and area under the curve of ½ peak intensity were significantly different ( P <0.05). In PC-3 xenografts, there were no differences in these four indicators. Fluorescent imaging indicated that targeted nanobubbles had an aggregation ability in C4-2 xenograft tumors. In conclusion, targeted nanobubbles carrying the anti-PSMA A10-3.2 aptamer have a targeted imaging effect in prostate cancer.

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“…Alibolandi et al (2015) show high levels of ECAM in colorectal cancer cells, which can be a therapeutic target of the cancer (Liao et al, 2015). One of its functions is to form Tj complexes to provide structural support for epithelial monolayers.…”

Section: Discussionmentioning

confidence: 99%

Epithelial cell adhesion molecule‐1 (ECAM1) is required in the maintenance of corneal epithelial barrier integrity

Zhou

Jiang

Wang

et al. 2015

Cell Biology International

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Corneal epithelial barrier integrity is critical in the maintenance of the corneal homeostasis. The corneal barrier dysfunction may be associated with the pathogenesis of a number of eye diseases. In this study, we assessed the expression of epithelial cell adhesion molecule-1 (ECAM1) in human corneal epithelial cells (HCE). The epithelial barrier function of the corneal epithelial monolayer was determined in Transwells. We found that the HCE cells expressed ECAM1. Knockdown of ECAM1 markedly compromised the HCE monolayer barrier function. A complex of ECAM1, claudin1, and occludin was detected in the HCE monolayers, which was not detected in the ECAM1-null HCE monolayers. Exposure to the proinflammatory cytokine, interleukin-13, inhibited the expression of ECAM1 in HCE cells and compromised the barrier function, which was prevented in the HCE monolayer with the ECAM1 overexpression. In conclusion, ECAM1 is required in the formation of the tight junction complex and maintaining the corneal epithelial barrier function.

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In vitro and in vivo evaluation of therapy targeting epithelial-cell adhesion-molecule aptamers for non-small cell lung cancer

Cited by 126 publications

References 57 publications

Engineering Polymersomes for Diagnostics and Therapy

Engineering Polymersomes for Diagnostics and Therapy

Diagnosis of prostate cancer using anti-PSMA aptamer A10-3.2-oriented lipid nanobubbles

Epithelial cell adhesion molecule‐1 (ECAM1) is required in the maintenance of corneal epithelial barrier integrity

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