In Vitro and in Vivo Antiviral Activity of Mizoribine Against Foot-And-Mouth Disease Virus

Li, Shifang; Gong, Mei-jiao; Sun, Yinyan; Shao, Jing; Zhang, Yongguang; Chang, Huiyun

doi:10.3390/molecules24091723

Cited by 11 publications

(11 citation statements)

References 24 publications

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“…This plant has also been tested against papillomavirus type 1 (PV-1) 52 and herpes simplex virus (HSV) 53 using polysaccharides. In addition, aqueous extracts of this herbal medicine have been shown to be effective against foot-and-mouth disease 24 , 54 . Furthermore, flavonoids from this species of Meliaceae inhibited the viral activity of several influenza strains (H 1 N 1 , H 1 N 2 , H 2 N 2 , H 2 N 3 , H 5 N 1 , H 7 N 2 , H 7 N 3 , H 7 N 7 and H 9 N 2 ) 55 .…”

Section: Discussionmentioning

confidence: 99%

In vitro antiviral effect of ethanolic extracts from Azadirachta indica and Melia azedarach against goat lentivirus in colostrum and milk

Sousa

Pinheiro

Araújo³

et al. 2023

Sci Rep

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This study aimed to evaluate, in vitro, the use of leaf extracts of Azadirachta indica (A. indica) and Melia azedarach (M. azedarach) as antivirals against caprine lentivirus (CLV) in colostrum and milk of goat nannies. These were collected from eight individuals and infected with the standard strain of CLV. Samples were then subdivided into aliquots and treated with 150 µg/mL of crude extract, and with ethyl acetate and methanol fractions for 30, 60, and 90 min. Next, somatic cells from colostrum and milk were co-cultured with cells from the ovine third eyelid. After this step, viral titers of the supernatants collected from treatments with greater efficacy in co-culture were assessed. The organic ethyl acetate fractions of both plants at 90 min possibly inhibited the viral activity of CLV by up to a thousandfold in colostrum. In milk, this inhibition was up to 800 times for the respective Meliaceae. In conclusion, the ethanolic fraction of ethyl acetate from both plants demonstrated efficacy against CLV in samples from colostrum and milk when subjected to treatment, which was more effective in colostrum.

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Section: Discussionmentioning

confidence: 99%

In vitro antiviral effect of ethanolic extracts from Azadirachta indica and Melia azedarach against goat lentivirus in colostrum and milk

Sousa

Pinheiro

Araújo³

et al. 2023

Sci Rep

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“…intracellular GTP pools [12,13,18]. Therefore, the mechanism of MZR against BKPyV may be also involve inhibition of IMPDH, further in Vitro and in Vivo experiments are needed.…”

Section: Discussionmentioning

confidence: 99%

“…During the conversion from MMF to MZR, the lower risk of virus infection might be associated with the reduced intensity of immunosuppression, which facilitates the immune system to kill the virus. Significantly, in vitro and in vivo studies showed that MZR could inhibit the replication of CMV, hepatitis C virus, and foot-and-mouth disease virus [11][12][13]. Previous studies have also suggested conversion from MMF to MZR correlated with lower BKPyV viruria/viremia [14,15].…”

mentioning

confidence: 97%

Conversion from mycophenolate mofetil to mizoribine in the early stages of BK polyomavirus infection could improve kidney allograft prognosis: a single-center study from China

Cheng

Wen

et al. 2021

BMC Nephrol

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Background Some studies have suggested mizoribine (MZR) could inhibit the replication of BK polyomavirus (BKPyV). The purpose of this study was to explore whether conversion from mycophenolate mofetil (MMF) to MZR in the early stages of BKPyV infection can improve kidney allograft prognosis. Methods Twenty-one kidney transplant recipients with BKPyV viruria/viremia and ten with BK polyomavirus-associated allograft nephropathy (BKPyVAN) received MZR conversion therapy were retrospectively identified. The clearance rate of urine and blood BKPyV DNA, change of serum creatinine (SCr), uric acid (UA), hemoglobin (HB), white blood cell (WBC), lymphocyte ratio, platelet (PLT), routine urinalysis, panel reactive antibody (PRA), and gastrointestinal disorders during follow-up of the 2 groups were evaluated and compared. Results After MZR conversion therapy, the clearance rate of urine and blood viral load in BKPyV viruria/viremia group were 85.7 and 100 %, while that in BKPyVAN were 40 and 87.5 %, respectively. Stable SCr were observed in all cases of BKPyV viruria/viremia group, while that of BKPyVAN was only 40 % (P < 0.001) and one even progressed to end-stage renal disease. The results of routine urinalysis in the two groups showed no significant changes before and after MZR conversion therapy. However, in BKPyV viruria/viremia group, four cases developed acute rejection and one had positive PRA-II but no donor specific antibody, requiring conversion back to MMF. Hyperuricemia was the common adverse effect of MZR. Conclusions Conversion from MMF to MZR could help clear BKPyV infection. As compared to BKPyVAN, patients who underwent initiation of MZR conversion therapy in the early stages of BKPyV infection maintained stable allograft function. Prospective studies with larger sample size are needed to ascertain this preliminary finding.

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“…cDNAs were synthesized using a PrimeScript™ RT reagent kit containing gDNA Eraser with random hexamers. The synthesized cDNAs were subjected to Q-PCR analysis using specific primers as described previously (Li et al, 2019). Q-PCR was performed in triplicate on an Agilent Technologies Stratagene Mx3005 P instrument (Agilent, USA) using SYBR Premix Ex Taq™ II (Tli RNaseH Plus) in a 25 μL reaction mixture.…”

Section: Rna Extraction and Q-pcr Analysismentioning

confidence: 99%

Antiviral activity of merimepodib against foot and mouth disease virus in vitro and in vivo

Li¹,

Gong²,

Shao³

et al. 2019

Molecular Immunology

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Foot and mouth disease virus (FMDV), a member of family Picornaviridae, belongs to the genus Aphthovirus, which causes foot and mouth disease (FMD), a highly transmissible disease that affects cloven-hoof animals. In spite of the fact that efficient vaccines are available, effective antiviral molecules for FMD are needed to reduce viral infection during early stages of infection. In this study, merimepodib was found to efficiently inhibit FMDV replication in a dose-dependent manner. The 50% inhibitory concentration (IC 50 ) of merimepodib antiviral activity against two distinct FMDV strains (O/MYA98/BY/2010 and A/GD/MM/CHA/2013) was estimated to be 7.859 and 2.876 μM, respectively, while the 50% cytotoxic concentration (CC 50 ) of merimepodib was found to be 47.74 μM. Furthermore, treatment with 30 μg merimepodib efficiently prolonged the survival time of suckling mice infected with FMDV. Taken together, these results suggested that merimepodib has the potential to be a novel antiviral agent against FMDV.

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In Vitro and in Vivo Antiviral Activity of Mizoribine Against Foot-And-Mouth Disease Virus

Cited by 11 publications

References 24 publications

In vitro antiviral effect of ethanolic extracts from Azadirachta indica and Melia azedarach against goat lentivirus in colostrum and milk

In vitro antiviral effect of ethanolic extracts from Azadirachta indica and Melia azedarach against goat lentivirus in colostrum and milk

Conversion from mycophenolate mofetil to mizoribine in the early stages of BK polyomavirus infection could improve kidney allograft prognosis: a single-center study from China

Antiviral activity of merimepodib against foot and mouth disease virus in vitro and in vivo

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