In vitro and in vivo behaviour of Ca- and P-enriched anodized titanium

Fini, Milena; Cigada, A.; Rondelli, G.; Chiesa, Roberto; Giardino, Roberto; Giavaresi, Gianluca; Aldini, N. Nicoli; Torricelli, Paola; Vicentini, B.

doi:10.1016/s0142-9612(99)00060-5

Cited by 180 publications

(108 citation statements)

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“…To achieve this goal, many commercially available implants use bioactive materials, such as calcium phosphate ceramics. Many reports have shown that modifying an implant surface with biological materials can enhance protein adsorption and cell attachment, as well as promote bone formation 24,25) . In principle, only a few surface parameters may affect cell responses on coated surfaces, including surface morphology and roughness, chemical composition, wettability, and electric charge 15,18,26) .…”

Section: Discussionmentioning

confidence: 99%

Enhanced biological responses of a hydroxyapatite/TiO<Sub>2</Sub> hybrid structure when surface electric charge is controlled using radiofrequency sputtering

et al. 2012

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We investigated whether surface roughness and control of surface electric charge of a hydroxyapatite (HA)/titanium oxide (TiO2) hybrid coating could enhance biological responses associated with bone formation. After acid etching, a titanium surface was modified with HA and TiO2 by the dual sputtering deposition technique using radiofrequency sputtering. These surfaces were analyzed for surface roughness and surface electric charge intensity. Rat bone marrow-derived osteoblast-like cells were cultured on HA/TiO2 hybrid surfaces with different electric charges. The attachment and spreading behavior of these cells were significantly increased on the hybrid surface (p<0.05). In vivo experiment, the strength of bone-titanium implant integration with a hybrid surface was 3 times that of a control (p<0.05). The dual sputtering deposition technique created a HA/TiO2 hybrid structure. Our results show that the surface electric charge on a titanium surface is an important factor for enhancing biological responses.

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Section: Discussionmentioning

confidence: 99%

Enhanced biological responses of a hydroxyapatite/TiO<Sub>2</Sub> hybrid structure when surface electric charge is controlled using radiofrequency sputtering

et al. 2012

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“…This test was performed by direct contact of silicone rubber elastomer with cultured fibroblast cells as described earlier. 15,16 Mouse fibroblast L929 cell lines were cultured in Dulbecco modified Eagle's medium (DMEM; GIBCO, Grand Island, Ny, USA), with 10% fetal calf serum, penicillin/streptomycin (100/100 U), and gentamycin (100 mg/ml), and incubated in 5% CO 2 at 371C for 24 h. Then, cell cultures were incubated with samples or negative (DMEM) and positive controls (0.1% phenol solution) at the same conditions for 24 h. The viability and cell metabolic activity were tested by the MTT assays. At least three replicates were performed for this test.…”

Section: Cytotoxicity Tests Of the Vitreous Capsule Materialsmentioning

confidence: 99%

A new strategy to replace the natural vitreous by a novel capsular artificial vitreous body with pressure-control valve

Gao

et al. 2007

Eye

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Purpose The current vitreous substitutes such as silicone oil, heavy silicone oil, and polymeric gels that are directly injected into vitreous cavity frequently cause severe intraocular complications. There is a very urgent need to find a more suitable artificial vitreous substitute for pars plana vitrectomy (PPV) surgery. Methods We have devised a novel capsular artificial vitreous using tailor-made silicone rubber elastomer. The novel device was implanted into the vitreous cavity of rabbit after PPV and the eye was examined by ophthalmoscopy, fundus photography, and tonometry during an 8-week treatment period. B-scan ultrasonography, electroretinogram (ERG), and histological studies by light microscopy were also performed at the end of 8 weeks.Results The novel artificial vitreous body consists of a thin vitreous-like capsule with a silicone tube-valve system. The capsule can be folded and implanted into vitreous cavity through 1.5 mm incision on sclera. Physiological balanced solution (PBS) was then injected into the capsule and inflated to support retina and control intraocular pressure (IOP) through the tube-valve system subsequently fixed under the conjunctiva. Experiments using rabbits showed that the novel vitreous body could effectively support the retina and apparently induced no significant pathological changes in the eye over 8 weeks. Conclusion This approach may provide a new research strategy in the vitreous replacement technology. The novel artificial vitreous body device can effectively support retina, control IOP, and has good biocompatibility. It may be a good alternative to injecting artificial vitreous although its tamponade properties and usefulness still have to be proven in complex vitreoretinal diseases.

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“…In an effort to improve osseointegration, the composition and topography of the implant surface were modified 8) . A Ti implant's surface is generally modified by increasing the surface roughness of the Ti surface or by altering the crystal structure and chemical composition by techniques such as oxidation methods [9][10][11][12] , anodic plasma-chemical treatments 13) , and calcium phosphate and hydroxyapatite coatings on the Ti surface [14][15][16][17][18] . Anodic oxidation is also used for the surface treatment of commercial implants to improve the implants' functional properties for clinical success.…”

Section: Introductionmentioning

confidence: 99%

Ultra-structural evaluation of an anodic oxidated titanium dental implant

Yamagami¹,

Nagaoka

Nakamura

et al. 2014

Dent. Mater. J.

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Anodic oxidation is used for the surface treatment of commercial implants to improve their functional properties for clinical success. Here we conducted ultrastructural and chemical investigations into the micro-and nanostructure of the anodic oxide film of a titanium implant. The anodic oxidized layer of a Ti6Al4V alloy implant was examined ultrastructurally by transmission electron microscopy (TEM) and scanning electron microscopy (SEM). They were also analyzed using energy dispersive X-ray spectrometry (EDS) and X-ray photoelectron spectroscopy (XPS). The TEM revealed that the oxide layer of the Ti6Al4V implant prepared through anodic oxidation was separated into two layers. Al and V were not present on the top surface of the anodic oxide. This can be attributed to the biocompatibility of the anodic oxidized Ti6Al4V alloy implant, because the release of harmful metal ions such as Al and V can be suppressed by the biocompatibility.

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In vitro and in vivo behaviour of Ca- and P-enriched anodized titanium

Cited by 180 publications

References 0 publications

Enhanced biological responses of a hydroxyapatite/TiO<Sub>2</Sub> hybrid structure when surface electric charge is controlled using radiofrequency sputtering

Enhanced biological responses of a hydroxyapatite/TiO<Sub>2</Sub> hybrid structure when surface electric charge is controlled using radiofrequency sputtering

A new strategy to replace the natural vitreous by a novel capsular artificial vitreous body with pressure-control valve

Ultra-structural evaluation of an anodic oxidated titanium dental implant

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