In-vitro and in-vivo wound healing studies of Ag@TiO₂NRs/GG hydrogel film for skin tissue regeneration

Abstract

One million cases of skin wounds, either closed or open wounds, necessitate wound treatments to improve the quality of life. In this study, gellan gum biopolymer (Ag@TiO2NRs/GG) hydrogel film with Ag loaded TiO2 nanorods was fabricated for wound healing dressing. The wound healing performance of Ag@TiO2WR/GG hydrogel was tested in vitro and in vivo to investigate its ability to regenerate skin tissue. FTIR, XRD, and SEM were used to examine the physical and chemical properties of prepared Ag@TiO2NRs/GG hydrogel film, as well as pure Ag and Ag@TiO2NRs. The FTIR spectra revealed the functional groups of Ag, TiO2NRs, GG, and their interactions. The hydrogel film was in an amorphous form, according to XRD analysis, due to the helical structure of GG and the presence of Ag and TiO2NRs in distinct phases The SEM image shows agglomeration of Ag particles and elongated TiO2 nanorods, indicating that Ag@TiO2NRs were successfully incorporated onto GG hydrogel film. Human skin fibroblast cells (CRL2522) were used to study the in vitro wound healing of Ag@TiO2NRs/GG hydrogel film for cell viability and proliferation. After 72 h, ~98,022 cells/well were counted, indicating that the Ag@TiO2NRs/GG was biocompatible and non-toxic. In vivo wound healing on Sprague Dawley rats revealed 100% wound healing after 14 days of treatment with Ag@TiO2NRs/GG hydrogel film. On a treated skin wound, ultrasound images revealed a thicker epidermis, clear dermis, and subcutis layer, indicating a positive correlation between wound healing and skin tissue regeneration.

Nanoparticles incorporated hydrogels for delivery of antimicrobial agents: developments and trends

“…224 In another study, gellan gum biopolymer hydrogel film with Ag-loaded TiO 2 nanorods (Ag@TiO 2 NRs/GG) based wound healing dressing was developed for evaluation of skin tissue regeneration. 225 The results of this study reported 100% wound healing using Ag@TiO 2 NRs/GG hydrogel as revealed through clear dermis, thicker epidermis, and subcutis layer after 14 days of treatment. Furthermore, MgO nanoparticles doped cellulose hydrogel were developed for pH triggered sustained release of doxorubicin and demonstrated efficient antibacterial and anti-cancer effects.…”

Advances in Functionalization of Bioresorbable Nanomembranes and Nanoparticles for Their Use in Biomedicine

“…The films are gas permeable and prevent bacteria from entering the wound site [ 241 , 242 ]. In light of this, a gellan gum (GG)-based hydrogel film (Ag@TiO 2 NRs/GG) containing Ag-loaded TiO 2 nanorods (TiO 2 NRs) was recently published [ 237 ] ( Figure 5 b). TiO 2 NRs, which have an elongated structure and are considered cutting-edge one-dimensional fillers, were utilized in the manufacturing process of hydrogel film.…”