In Vitro and In Vivo Assessment of<i>Salmonella enterica</i>Serovar Typhimurium DT104 Virulence

Allen, C.; Fedorka–Cray, Paula J.; Vázquez‐Torres, Andrés; Suyemoto, M. Mitsu; Altier, Craig; Ryder, L. R.; Fang, Ferric C.; Libby, Stephen J.

doi:10.1128/iai.69.7.4673-4677.2001

Cited by 42 publications

(42 citation statements)

References 24 publications

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“…With regard to invasion capacity, however, it is interesting to note that S. enterica Typhimurium DT104 infection resulted in a higher egg contamination rate than S. enterica Enteritidis PT4 [54]. In the mouse model of infection, multidrug-resistant DT104 strains did not reveal evidence of enhanced nor reduced virulence when compared with ATCC S. enterica Typhimurium strain 14028s [2].…”

Section: Change In Virulence?mentioning

confidence: 96%

“…The hypothesis of hypervirulence of multidrug-resistant S. enterica Typhimurium DT104 has been investigated by testing invasiveness in cell lines and virulence in the mouse model of systemic salmonellosis [2,13,14]. However, the strains tested did not appear more invasive than non-resistant cohorts and some DT104 isolates appeared even less invasive than non-resistant relatives [13].…”

Section: Change In Virulence?mentioning

confidence: 99%

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Molecular characterization, spread and evolution of multidrug resistance in Salmonella enterica Typhimurium DT104

2001

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-Multidrug-resistant Salmonella enterica serovar Typhimurium phage type DT104 has emerged during the last decade as a global health problem because of its involvement in diseases in animals and humans. Multidrug-resistant DT104 strains are mostly resistant to ampicillin, chloramphenicol, streptomycin, sulfonamides and tetracyclines (ACSSuT resistance type). The genes coding for such resistances are clustered on the chromosome. This paper reviews new developments in the characterization of S. enterica Typhimurium DT104, its chromosomal antibiotic resistance genes and their spread among other S. enterica Typhimurium phage types and other S. enterica serovars, the development of specific detection methods, virulence characteristics, and the evolution of multidrugresistance with regard to the emergence of quinolone resistance.Salmonella Typhimurium DT104 / multidrug resistance / Salmonella genomic island I Résumé -Caractérisation moléculaire, diffusion et évolution de la résistance multiple aux antibiotiques chez Salmonella enterica Typhimurium DT104. Le lysotype DT104 de Salmonella enterica Typhimurium présentant une résistance multiple aux antibiotiques a émergé durant la dernière décennie comme un problème mondial de santé publique qui touche à la fois humains et animaux. Les souches du lysotype DT104 sont en général résistantes à l'ampicilline, au chloramphénicol, à la streptomycine, aux sulphamides et aux tétracyclines (profil de résistance ACSSuT). Les gènes codant pour ces résistances sont groupés sur le chromosome. Cet article présente une revue des derniers dévelop-pements dans la caractérisation de S. enterica Typhimurium DT104, ses gènes chromosomiques de résis-tance et leur diffusion chez d'autres lysotypes de S. enterica Typhimurium et d'autres serotypes de S. enterica, le développement de méthodes spécifiques de détection, les caractéristiques de virulence, et l'évolution de la résistance multiple au regard de l'émergence de la résistance aux quinolones.Salmonella Typhimurium DT104 / résistance multiple / îlot génomique I Vet. Res. 32 (2001) [301][302][303][304][305][306][307][308][309][310] 301

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Section: Change In Virulence?mentioning

confidence: 96%

Section: Change In Virulence?mentioning

confidence: 99%

Molecular characterization, spread and evolution of multidrug resistance in Salmonella enterica Typhimurium DT104

2001

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“…Mice have been used previously as a model to assess virulence of serovar Typhimurium for humans (1). Highly virulent murine strains, however, do not necessarily have to be virulent in humans and vice versa (e.g., S. enterica serovar Typhi).…”

Section: Discussionmentioning

confidence: 99%

“…It was the aim of the present study to determine the virulence of pigeon-associated Salmonella strains for humans by using epidemiological data, in vitro enterocyte and macrophage models, and an in vivo murine model. The in vitro and in vivo models have been previously used to assess virulence of Salmonella strains for humans (1).…”

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confidence: 99%

Assessment of Virulence of Pigeon Isolates of Salmonella enterica subsp. enterica Serovar Typhimurium Variant Copenhagen for Humans

et al. 2004

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Salmonella enterica serovar Typhimurium variant Copenhagen was isolated from 5 of 152 (3.3%) feral pigeons from the city of Ghent (Belgium) and from 26 pooled fecal samples from 114 pigeon lofts (22.8%). These isolates belonged to phage type (PT) 99. Seven of the pigeon isolates were further compared in vitro to five human variant Copenhagen isolates, 2 isolates of PT 208, 1 isolate each of PT 120 and U302, and a nontypeable isolate. No differences in invasiveness in human intestinal epithelial Caco-2 cells were found. The human strains, however, were able to multiply significantly more inside human THP-1 macrophages than the pigeon strains. After inoculation of mice with a pigeon PT 99 strain, high numbers of Salmonella bacteria were shed with the feces, the internal organs were heavily colonized, and the animals showed severe clinical symptoms resulting in death. In conclusion, the less-pronounced ability of the pigeon variant Copenhagen strains to multiply inside human macrophages than human strains as well as the lack of human PT 99 isolates during 2002, despite the relatively high frequency of this PT in the pigeon population, suggest these strains to be of low virulence to humans. However, the high virulence for mice of the tested strain implies that rodents may act as reservoirs.Because of the high numbers of free-roaming and captive pigeons in Belgium and their well known association with Salmonella, pigeons might be a source of salmonellosis in humans. Pigeon isolates of Salmonella enterica subsp. enterica serovar Typhimurium variant Copenhagen are considered a hostadapted lineage of the serovar Typhimurium (5, 6, 8). As might be expected from host restricted Salmonella strains (3, 10), these pigeon isolates are highly virulent to pigeons (6, 7). Adaptation to one host often coincides with low virulence to other hosts, such as humans (3, 10). It was the aim of the present study to determine the virulence of pigeon-associated Salmonella strains for humans by using epidemiological data, in vitro enterocyte and macrophage models, and an in vivo murine model. The in vitro and in vivo models have been previously used to assess virulence of Salmonella strains for humans (1). MATERIALS AND METHODSPrevalence of pigeon-adapted variant Copenhagen strains in pigeons and humans. For the pigeon strains, pooled fecal samples from 114 different pigeon lofts in Belgium and pooled samples from the intestines, livers, and spleens of 152 feral pigeons from the city of Ghent (Belgium) were examined for the presence of Salmonella. The samples were enriched overnight in tetrathionate brilliant green broth (Merck, Darmstadt, Germany) at 37°C and then streaked onto brilliant green agar (Lab-M, Bury, United Kingdom). Colonies with characteristics of Salmonella were further identified biochemically on the basis of glucose fermentation, sulfur reduction, the presence of lysine decarboxylation, the absence of lactose fermentation, and urease production. The isolates were serotyped by means of agglutination tests by using single-fa...

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“…The survival in phagocytes is an important step to induce septicemia in pigs, causing clinical signs similar to those in humans (16,31); therefore, this animal model may be used to study human salmonellosis. Salmonella is able to survive and replicate in phagocytic cells, and this is an essential component of the virulence of these bacteria (1). S. enterica serovar Typhimurium cells that have invaded the macrophage by phagocytosis are able to replicate intracellularly (28) and induce apoptosis (32).…”

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confidence: 99%

Interaction between Host Cells and Septicemic Salmonella enterica Serovar Typhimurium Isolates from Pigs

et al. 2009

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Salmonella enterica serovar Typhimurium is an important pathogen in swine and is also a frequently reported zoonotic agent. The objective of this study was to characterize isolates of S. enterica serovar Typhimurium associated with septicemia in swine and to compare them to isolates recovered from clinically healthy pigs. We were particularly interested in comparing the two groups of isolates for their ability to adhere to and invade host cells, to be phagocytized and survive in monocyte cells, to induce apoptosis, and to adhere to intestinal mucus. Their surface properties were also evaluated by interactions with solvents. The isolates recovered from diseased animals were shown to invade intestinal epithelial cell lines at a higher rate (P ‫؍‬ 0.003) than isolates from healthy pigs. Septicemic isolates were phagocytized by human monocytes at a higher rate than isolates from healthy pigs (P ‫؍‬ 0.009). The mean percentages of phagocytosis were significantly lower for human monocytes than for porcine monocytes (P ‫؍‬ 0.02 and P ‫؍‬ 0.008, respectively) for isolates from both diseased and healthy animals. Healthy animal isolates were phagocytized more by porcine monocytes at 15 min (P ‫؍‬ 0.02) than septicemic isolates. No difference between isolates from septicemic pigs and isolates from healthy pigs was detected for other tested parameters. These results suggest that septicemic isolates have a particular pattern of invasion.

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In Vitro and In Vivo Assessment ofSalmonella entericaSerovar Typhimurium DT104 Virulence

Cited by 42 publications

References 24 publications

Molecular characterization, spread and evolution of multidrug resistance in Salmonella enterica Typhimurium DT104

Molecular characterization, spread and evolution of multidrug resistance in Salmonella enterica Typhimurium DT104

Assessment of Virulence of Pigeon Isolates of Salmonella enterica subsp. enterica Serovar Typhimurium Variant Copenhagen for Humans

Interaction between Host Cells and Septicemic Salmonella enterica Serovar Typhimurium Isolates from Pigs

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